{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:13:00Z","timestamp":1790802780291,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,2,28]],"date-time":"2003-02-28T00:00:00Z","timestamp":1046390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:p>It has been reported that matrix metalloproteinase (MMP) produced by host cells plays a major role in periodontal tissue destruction. In addition, secreted virulence factors from <jats:italic>Porphyromonas gingivalis<\/jats:italic> can alter MMP secretion and cause activation in host cells that lead to the tissue degradation. In this study, we examine the effects of <jats:italic>P.\u2003gingivalis<\/jats:italic> supernatant on matrix metalloproteinase\u20102 (MMP\u20102) activation in human periodontal ligament (HPDL) cells. Cultures of HPDL cells were treated with <jats:italic>P.\u2003gingivalis<\/jats:italic> supernatant for 48\u2003h and the level of MMP\u20102 activation was monitored by gelatin zymography. The profound activation of MMP\u20102 was seen only in the treated group. The activation of MMP\u20102 was inhibited by MMP inhibitors phenanthroline and EDTA, but not serine protease or cysteine protease inhibitors. To study the correlation between the expression of membrane\u2010type\u20101 matrix metalloproteinase (MT1\u2010MMP) and the activation of MMP\u20102, the level of MT1\u2010MMP was analyzed. The results from reverse\u2010transcription polymerase chain reaction (RT\u2010PCR) and Western analysis indicated that <jats:italic>P.\u2003gingivalis<\/jats:italic> supernatant up\u2010regulated the expression of MT1\u2010MMP in both transcription and translation levels within 48\u2003h. These results suggest that <jats:italic>P.\u2003gingivalis<\/jats:italic> supernatant can activate MMP\u20102 in HPDL cells and the mechanism of activation may involve the increased amount of MT1\u2010MMP. It is possible that the activation of MMP\u20102 by <jats:italic>P.\u2003gingivalis<\/jats:italic> plays a role in the process of chronic periodontitis.<\/jats:p>","DOI":"10.1034\/j.1600-0765.2003.01650.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T08:55:58Z","timestamp":1047459358000},"page":"115-121","source":"Crossref","is-referenced-by-count":51,"title":["Activation of MMP\u20102 by <i>Porphyromonas gingivalis<\/i> in human periodontal ligament cells"],"prefix":"10.1111","volume":"38","author":[{"given":"Kassara","family":"Pattamapun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siriluck","family":"Tiranathanagul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tussanee","family":"Yongchaitrakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jintakorn","family":"Kuwatanasuchat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasit","family":"Pavasant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,2,28]]},"reference":[{"key":"e_1_2_15_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1986.tb00849.x"},{"key":"e_1_2_15_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1988.tb01590.x"},{"key":"e_1_2_15_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb02113.x"},{"key":"e_1_2_15_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00194.x"},{"key":"e_1_2_15_6_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.5s.474"},{"key":"e_1_2_15_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(98)80035-5"},{"key":"e_1_2_15_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345940730080201"},{"key":"e_1_2_15_9_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.3.353"},{"key":"e_1_2_15_10_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1601-0825.2001.70110.x"},{"key":"e_1_2_15_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1994.tb00272.x"},{"key":"e_1_2_15_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(98)90123-8"},{"key":"e_1_2_15_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(99)00032-3"},{"key":"e_1_2_15_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.11.5872"},{"key":"e_1_2_15_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(98)90110-X"},{"key":"e_1_2_15_16_2","first-page":"151","article-title":"Activation mechanisms of matrix metalloproteinases","volume":"378","author":"Nagase H","year":"1997","journal-title":"Biol Chem"},{"key":"e_1_2_15_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.51.30479"},{"key":"e_1_2_15_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.2.801"},{"key":"e_1_2_15_19_2","doi-asserted-by":"publisher","DOI":"10.1067\/moe.2001.115136"},{"key":"e_1_2_15_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00049520"},{"key":"e_1_2_15_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.7.574"},{"key":"e_1_2_15_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00666168"},{"key":"e_1_2_15_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1995.tb00194.x"},{"key":"e_1_2_15_24_2","doi-asserted-by":"crossref","first-page":"4940","DOI":"10.1128\/iai.64.12.4940-4945.1996","article-title":"Effect of Porphyromonas gingivalis on epithelial cells MMP\u20109 type IV collagenase production","volume":"64","author":"Fravalo P","year":"1996","journal-title":"Infect Immun"},{"key":"e_1_2_15_25_2","doi-asserted-by":"crossref","first-page":"4931","DOI":"10.1128\/iai.65.12.4931-4936.1997","article-title":"Bacterial phospholipase C upregulates matrix metalloproteinase expression by cultured epithelial cells","volume":"65","author":"Firth JD","year":"1997","journal-title":"Infect Immun"},{"key":"e_1_2_15_26_2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1128\/iai.57.1.213-218.1989","article-title":"A protease of Bacteroides gingivalis degrades cell surface and matrix glycoproteins of cultured gingival fibroblasts and induces secretion of collagenase and plasminogen activator","volume":"57","author":"Uitto V\u2010J","year":"1989","journal-title":"Infect Immun"},{"key":"e_1_2_15_27_2","doi-asserted-by":"crossref","first-page":"4491","DOI":"10.1128\/iai.60.11.4491-4495.1992","article-title":"Identification of proteases from periopathogenic bacteria as activators of latent human neutrophil and fibroblast\u2010type interstitial collagenases","volume":"60","author":"Sorsa T","year":"1992","journal-title":"Infect Immun"},{"key":"e_1_2_15_28_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760060501"},{"key":"e_1_2_15_29_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345850640080101"},{"key":"e_1_2_15_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00389-4"},{"key":"e_1_2_15_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.10.5331"},{"key":"e_1_2_15_32_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3340345"},{"key":"e_1_2_15_33_2","doi-asserted-by":"crossref","first-page":"2789","DOI":"10.1242\/jcs.111.18.2789","article-title":"The activation of proMMP\u20102 (gelatinase A) by HT1080 fibrosarcoma cells is promoted by culture on a fibronectin substrate and is concomitant with an increase in processing of MT1\u2010MMP (MMP\u201014) to a 45 kDa form","volume":"111","author":"Staton H","year":"1998","journal-title":"J Cell Sci"},{"key":"e_1_2_15_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.4.1176-1182.1995"},{"key":"e_1_2_15_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.11.7482"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2003.01650.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2003.01650.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0765.2003.01650.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T10:36:17Z","timestamp":1697193377000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0765.2003.01650.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,2,28]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1034\/j.1600-0765.2003.01650.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0765.2003.01650.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,2,28]]}}}