{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:51:19Z","timestamp":1785797479508,"version":"3.56.0"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,6,26]],"date-time":"2003-06-26T00:00:00Z","timestamp":1056585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Periodontitis is characterized by altered bone turnover, but local measurements are difficult.<\/jats:p><jats:p><jats:bold>Objectives:\u2002<\/jats:bold> The objective of this study was to develop a method to measure multiple markers of bone turnover from single samples collected at various bone surfaces of the periodontium, and to test the ratios of these markers against more traditional serum and gingival crevicular fluid (GCF) samples.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> Fourteen subjects with untreated periodontitis were recruited for sampling serum, GCF (from sites \u2265\u200a5\u2003mm probing depth that bled on probing) and washes of periodontal bone surfaces (adjacent interproximal, vestibular cortical and trabecular bone) with a novel irrigating device. All samples were analyzed for osteocalcin (OC, bone turnover marker; RIA), cross\u2010linked N\u2010telopeptide of type I collagen (NTx, bone resorption marker; ELISA) and albumin (Alb, serum protein; ELISA). Results were reported as ratios: OC\/NTx to determine relative bone turnover, and OC\/Alb or NTx\/Alb to determine local OC or NTx production.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The OC\/NTx ratio was significantly higher (<jats:italic>p<\/jats:italic>\u2003\u2264\u20030.05) in serum vs. GCF (OC undetectable), interproximal bone and cortical vestibular bone, but significantly lower than in trabecular vestibular bone. The OC\/Alb ratio for serum was also statistically lower than for vestibular trabecular bone. The NTx\/Alb ratio for serum was statistically lower than for GCF and all the bone wash test sites. The results indicated considerable local production of both OC and NTx.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> This system demonstrated that multiple markers of bone turnover can be harvested by irrigation from periodontal bone microenvironments. Bone turnover profiles from periodontal bone surfaces and GCF differed from systemic bone turnover profiles (serum) and may be valuable in tracking site\u2010specific responses to disease or treatment.<\/jats:p>","DOI":"10.1034\/j.1600-0765.2003.02002.x","type":"journal-article","created":{"date-parts":[[2010,11,30]],"date-time":"2010-11-30T19:11:47Z","timestamp":1291144307000},"page":"355-361","source":"Crossref","is-referenced-by-count":19,"title":["Bone turnover markers in serum and periodontal microenvironments"],"prefix":"10.1034","volume":"38","author":[{"given":"A. N.","family":"Wilson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. B.","family":"Marx","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Reinhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,6,26]]},"reference":[{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650071119"},{"key":"e_1_2_13_3_2","first-page":"231","volume-title":"Principles of Bone Biology","author":"Ott SM","year":"1996"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1991.tb00088.x"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.9.865"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(00)00279-9"},{"key":"e_1_2_13_7_2","first-page":"337","volume-title":"Practical Non\u2010parametric Statistics","author":"Conover WJ","year":"1971"},{"key":"e_1_2_13_8_2","first-page":"49","article-title":"Gingival crevicular fluid: a new diagnostic aid in managing the periodontal patient","volume":"8","author":"Golub LM","year":"1976","journal-title":"Oral Sci Rev"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1997.2.1.123"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1994.tb00721.x"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1995.tb01793.x"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000110050193"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1997.68.4.385"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1994.tb00720.x"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.8.1045"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02383.x"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0501.1996.090602.x"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(99)00175-1"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00298992"},{"key":"e_1_2_13_20_2","first-page":"1472","article-title":"A cross\u2010sectional analysis of 5 different markers of collagen degradation in rheumatoid arthritis","volume":"25","author":"St. Clair EW","year":"1998","journal-title":"J Rheumatol"},{"key":"e_1_2_13_21_2","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1128\/iai.49.3.487-493.1985","article-title":"Relationship between gingival crevicular fluid and serum antibody titers in young adults with generalized and localized periodontitis","volume":"49","author":"Tew JG","year":"1985","journal-title":"Infect Immun"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1989.60.1.44"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1993.tb00400.x"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2003.02002.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0765.2003.02002.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T04:18:30Z","timestamp":1697343510000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0765.2003.02002.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,6,26]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,8]]}},"alternative-id":["10.1034\/j.1600-0765.2003.02002.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0765.2003.02002.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,6,26]]}}}