{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T08:31:49Z","timestamp":1788510709023,"version":"build-2803163510"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,10,4]],"date-time":"2002-10-04T00:00:00Z","timestamp":1033689600000},"content-version":"vor","delay-in-days":64,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The combination of cyclosporin\u2010A (CS\u2010A) and ultraviolet\u2010B (UV\u2010B) irradiation is not recommended in the treatment of psoriasis, because risks of UV\u2010B\u2010induced skin cancer are increased. The recommendation, however, has not well been confirmed by basic researches.<\/jats:p><jats:p><jats:bold>Purpose:<\/jats:bold> In this study, we investigated the effects of CS\u2010A on UV\u2010B\u2010induced repair DNA synthesis, apoptosis and p53 expression.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Following the short\u2010term administration of CS\u2010A (5 and 50\u2003mg\/kg\/day) or vehicle (V) alone, female BALB\/c mice, 8\u201310\u2003weeks old, were treated with UV\u2010B irradiation (100 and 500 mJ<jats:sup>2<\/jats:sup> cm) or tape stripping (TS). After the treatment, the effects of CS\u2010A on the increased rate of epidermal DNA synthesis were examined by using 5\u2032\u2010bromodeoxyuridine (BrdU) pulse\u2010labelling techniques. In separate experiments, the effects of CS\u2010A on the number of sunburn cells, nick\u2010end labelling<jats:sup>\u2003+\u2003<\/jats:sup> cells and p53<jats:sup>\u2003+<\/jats:sup> cells were examined 24\u2003h after UV\u2010B irradiation.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Cyclosporin\u2010A significantly suppressed the UV\u2010B\u2010induced increase in BrdU uptake, which occurs to repair DNA damage, while there were no significant effects on the stripping (S)\u2010induced increase or the rate of normal epidermal proliferation, which is not associated with any DNA injuries. The number of sunburn cells, nick\u2010end labelling<jats:sup>\u2003+\u2003<\/jats:sup> cells and p53<jats:sup>\u2003+\u2003<\/jats:sup> cells was significantly reduced by pretreatment with CS\u2010A.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Cyclosporin\u2010A interferes with the self\u2010 protective mechanisms involved in both repair and apoptotic removal of UV\u2010B\u2010induced DNA damage. The loss of p53 expression is responsible for the effects of CS\u2010A.<\/jats:p>","DOI":"10.1034\/j.1600-0781.2002.00765.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T18:35:51Z","timestamp":1047494151000},"page":"163-168","source":"Crossref","is-referenced-by-count":26,"title":["Cyclosporin\u2010A suppresses p53\u2010dependent repair DNA synthesis and apoptosis following ultraviolet\u2010B irradiation"],"prefix":"10.1111","volume":"18","author":[{"given":"N.","family":"Sugie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Danno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,10,4]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"587","volume-title":"Psoriasis","author":"Roenigk HH","year":"1998"},{"key":"e_1_2_5_3_2","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1001\/archderm.1989.01670160093019","article-title":"Risk assessment of PUVA and cyclosporine","volume":"125","author":"Stern RS","year":"1989","journal-title":"Arch Dermatol"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)80015-3"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(96)00153-4"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.1.274"},{"key":"e_1_2_5_7_2","first-page":"136","article-title":"Sunlight and sunburn in human skin cancer: p53, apoptosis, and tumor promotion","volume":"1","author":"Brash DE","year":"1996","journal-title":"J\u00a0Invest Dermatol Proc"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(83)70160-X"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1995.tb01196.x"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1970.tb06038.x"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-1811(92)90019-8"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1981.tb08987.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1998.02306.x"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1987.tb07401.x"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00474.x"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12263506"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/372773a0"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00090.x"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198709000-00021"},{"key":"e_1_2_5_20_2","first-page":"526","article-title":"Increased ultraviolet radiation\u2010induced skin cancers in cyclosporine\u2010treated mice","volume":"19","author":"Nelson EW","year":"1987","journal-title":"Transplant Proc"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198708000-00022"}],"container-title":["Photodermatology, Photoimmunology &amp; Photomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0781.2002.00765.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0781.2002.00765.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0781.2002.00765.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T08:06:32Z","timestamp":1697529992000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0781.2002.00765.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,8]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,8]]}},"alternative-id":["10.1034\/j.1600-0781.2002.00765.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0781.2002.00765.x","archive":["Portico"],"relation":{},"ISSN":["0905-4383","1600-0781"],"issn-type":[{"value":"0905-4383","type":"print"},{"value":"1600-0781","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,8]]}}}