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Formation of \u00b7OH was demonstrated by electron spin resonance (ESR) spectroscopy combined with spin\u2010trapping techniques. It was shown that \u00b7OH generated via the <jats:sc>F<\/jats:sc>enton reaction abstracts first a hydrogen atom from barbituric acid (BA) and forms intermediately a paramagnetic derivative of BA. After a second attack by another \u00b7OH, the BA radical is transformed into dialuric acid (DA), which autoxidizes via the alloxan radical (\u00b7ALX) to ALX. Superoxide radicals (\u00b7O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>) are formed during autoxidation of DA and \u00b7ALX. They are able to regenerate ferrous ions. As a result, traces of iron salts are capable of catalyzing the conversion of large amounts of BA into ALX. Several scavengers of \u00b7OH were tested with regard to their efficiency in preventing the transformation of BA into ALX. Of all the scavengers analyzed, melatonin was shown to be one of the most potent compounds.<\/jats:p>","DOI":"10.1034\/j.1600-079x.2002.02936.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T08:32:41Z","timestamp":1047457961000},"page":"239-247","source":"Crossref","is-referenced-by-count":16,"title":["Transformation of barbituric acid into alloxan by hydroxyl radicals: interaction with melatonin and with other hydroxyl radical scavengers"],"prefix":"10.1034","volume":"33","author":[{"given":"H. 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