{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:46:11Z","timestamp":1791103571812,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,9,5]],"date-time":"2002-09-05T00:00:00Z","timestamp":1031184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p> <jats:italic>Hammadeh ME, Ertan AK, Georg MT, Rosenbaum P, Schmidt W. Relationship between ovarian stimulation regimen and interleukin level in pre\u2010ovulatory follicular fluid and their effect on ICSI outcome. AJRI 2002; 48:255\u2013261 \u00a9 Blackwell Munksgaard, 2002<\/jats:italic> <\/jats:p><jats:p>PROBLEM: The aims of this study were (i) to identify the presence and concentration of interleukin\u20106 (IL\u20106), IL\u20108 and granulocyte\u2010macrophage colony\u2010stimulating factor (GM\u2010CSF) in pre\u2010ovulatory follicular fluid (FF) in patients undergoing controlled ovarian hyperstimulation regimen for intracytoplasmic sperm injection (ICSI) with recombinant human follicle stimulating hormone (rFSH) (G.1), hMG (G.2) or rFSH\/hMG (G.3) treatment and (ii) to determine whether controlled ovarian hyperstimulation stimulation regimen could affect the concentration of these investigated interleukins and as a consequences the ICSI outcome. (iii) Moreover, it was intended to clarify the association between these investigated parameters and the peripheral blood level of estradiol 17\u2010\u03b2, progesterone, leuteinizing hormone (LH) at the day of oocyte retrieval and (iv) finally to find out whether these interleukin concentrations in FF could be considered as an additional parameter for predicting fertilization and pregnancy outcome in ICSI patients.<\/jats:p><jats:p>MATERIAL AND METHODS: Interleukin\u20106, IL\u20108 and GM\u2010CSF concentrations were determined in the FF of 85 patients. The levels of these interleukins were measured by the enzyme\u2010linked immunosorbent assay (ELISA) technique, using commercially available kits.<\/jats:p><jats:p>RESULTS: Interleukin\u20106 (6.5\u2003\u00b1\u20034.6\u2003pg\/mL), IL\u20108 (222.9\u2003\u00b1\u2003163.4\u2003pg\/mL) and GM\u2010CSF (1.6\u2003\u00b1\u20032.7\u2003pg\/mL) were present in pre\u2010ovulatory FF in ICSI patients. No significant difference in the mean concentration of IL\u20106, IL\u20108 and GM\u2010CSF was found between the groups. Moreover, there was no correlation between these interleukin levels in FF and steroid hormone concentration in the peripheral blood.<\/jats:p><jats:p>CONCLUSIONS: Interleukin\u20106, IL\u20108 and GM\u2010CSF were found in FF of patients undergoing ovarian hyperstimulation for ICSI treatment. The stimulation protocols showed no influence on these interleukin levels in FF. Moreover, no correlation was found between these investigated interleukins and steroid hormone concentrations in peripheral blood or ICSI outcome. Therefore, the determination of interleukin level in FF of patients undergoing ovarian hyperstimulation for ICSI therapy could not be recommended as a prognostic factor for ICSI outcome.<\/jats:p>","DOI":"10.1034\/j.1600-0897.2002.01123.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:26:59Z","timestamp":1047346019000},"page":"255-261","source":"Crossref","is-referenced-by-count":9,"title":["Relationship between Ovarian Stimulation Regimen and Interleukin level in Pre\u2010ovulatory Follicular Fluid and Their Effect on ICSI Outcome"],"prefix":"10.1111","volume":"48","author":[{"given":"M.E.","family":"HAMMADEH","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.K.","family":"ERTAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.T.","family":"GEORG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"ROSENBAUM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"SCHMIDT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,9,5]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a137929"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-11-3-454"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a137670"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-129-6-3427"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a136268"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.2.736"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-124-5-2043"},{"key":"e_1_2_7_9_2","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/S0015-0282(16)55548-4","article-title":"Cytokines\u2010involvement in reproduction","volume":"58","author":"Ben Rafael Z","year":"1992","journal-title":"Fertil Steril"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod22.1.73"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod43.1.8"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)53756-X"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80565-Z"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj.41.83"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)54483-5"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod49.3.635"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-980022"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.130.3.1750"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod48.3.538"},{"key":"e_1_2_7_20_2","doi-asserted-by":"crossref","first-page":"3762","DOI":"10.1210\/endo.137.9.8756544","article-title":"Interleukin\u20108 expression and modulation in human preovulatory follicles and ovarian cells","volume":"137","author":"Arici A","year":"1996","journal-title":"Endocrinol"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0960241"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1040325"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)00433-0"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)56581-9"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019139"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod48.1.15"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)55079-1"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.79.2.633"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)81489-6"},{"key":"e_1_2_7_30_2","first-page":"38","article-title":"Effect of interleukin\u20106 on proliferation and follicle stimulating hormone\u2010induced estradiol production by bovine granulosa cells in vitro: dependence on size of follicle","volume":"49","author":"Alpizar E","year":"1993","journal-title":"Biol Reprod"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/2.4.245"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1997.tb00192.x"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(98)00267-2"},{"key":"e_1_2_7_34_2","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1093\/oxfordjournals.humrep.a138491","article-title":"Progesterone control interleukin\u20108 prod endometrium chorio\u2010decidual cells underlines role neutrophil menstruation parturitio","volume":"9","author":"Kelly RW","year":"1994","journal-title":"Hum Reprod"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/2.8.555"},{"key":"e_1_2_7_36_2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1093\/oxfordjournals.humrep.a137607","article-title":"Concentration of immunoreactive interleukin\u20101 and interleukin\u20102 in human pre\u2010ovulatory follicular fluid","volume":"7","author":"Wang LJ","year":"1992","journal-title":"Hum Reprod"},{"key":"e_1_2_7_37_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1093\/oxfordjournals.humrep.a138327","article-title":"Concentration of interleukin\u20101\u03b2 correlates with prostaglandine E2 an F2\u03b1 in human pre\u2010ovulatory follicular fluid","volume":"9","author":"Watanabe H","year":"1994","journal-title":"Hum Reprod"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02211808"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)81512-9"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1994.tb04809.x"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)49154-5"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1270363"},{"key":"e_1_2_7_43_2","unstructured":"NormanRJ WangLJ:Tumor necrosis factor \u03b1 immunoactivity in human follicular fluid and its effects on human granulosa\u2010lutein cells. 34th Annual Scientific Meeting The Endocrine Society of Australia Proceedings Sydney Australia 1991 p. 91."}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0897.2002.01123.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0897.2002.01123.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0897.2002.01123.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:28:32Z","timestamp":1697416112000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0897.2002.01123.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9,5]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,10]]}},"alternative-id":["10.1034\/j.1600-0897.2002.01123.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0897.2002.01123.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9,5]]}}}