{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T01:39:12Z","timestamp":1777340352462,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,12,9]],"date-time":"2002-12-09T00:00:00Z","timestamp":1039392000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>Fornari MC, Sarto A, Berardi VE, Mart\u00ednez MA, Rocha MG, Pasqualini S, Diez RA. Effect of ovaric hyper\u2010stimulation on blood lymphocyte subpopulations, cytokines, leptin and nitrite among patients with unexplained infertility. AJRI 2002; 48:394\u2013403 \u00a9 Blackwell Munksgaard, 2002<\/jats:p><jats:p>PROBLEM:\u2002To determine the effect of ovarian stimulation on TH1, TH2 and natural killer (NK) lymphocytes, plasma cytokines, leptin and nitrite levels.<\/jats:p><jats:p>METHODS:\u2002Women with reproductive failure were studied during the implantation window, at baseline (<jats:italic>n<\/jats:italic>=18) and under ovarian stimulation (gonadotropins + progesterone, <jats:italic>n<\/jats:italic>=6). Controls: eight fertile women. Lymphocyte subpopulations and NK function were determined by flow cytometry. Interleukin\u20102 (IL\u20102), IL\u20104, IL\u201010, IFN\u2010\u03b3, TNF\u2010\u03b1, TGF\u2010\u03b21 and leptin were measured by enzyme immunoassay (EIA); nitrite by the Griess reaction.<\/jats:p><jats:p>RESULTS:\u2002At baseline, patients had higher values of NK effectors, NK activity and plasma IFN\u2010\u03b3 and IL\u20102 than controls. Conversely, TGF\u2010\u03b21 values were lower. Hormones induced leukocytosis. Under stimulation, TH1\u2003CD4<jats:sup>+<\/jats:sup> cells, NK effectors and function and plasma IFN\u2010\u03b3 and IL\u20102 decreased, while transforming growth factor (TGF)\u2010\u03b21 increased. Other variables did not change.<\/jats:p><jats:p>CONCLUSION:\u2002The abnormal distribution of leukocytes, high TH1 cytokines and a low TGF\u2010\u03b21 associated with reproductive failure, respond to ovarian stimulation, achieving total or partial normalization.<\/jats:p>","DOI":"10.1034\/j.1600-0897.2002.01128.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:27:46Z","timestamp":1047346066000},"page":"394-403","source":"Crossref","is-referenced-by-count":16,"title":["Effect of Ovaric Hyper\u2010stimulation on Blood Lymphocyte Subpopulations, Cytokines, Leptin and Nitrite Among Patients with Unexplained Infertility"],"prefix":"10.1111","volume":"48","author":[{"given":"M. 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