{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:01:40Z","timestamp":1791295300281,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,9,5]],"date-time":"2002-09-05T00:00:00Z","timestamp":1031184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p> <jats:italic>Tokmad&amp;#x007a;\u0306i\u0107 VS, Tsuji Y, Bogovi\u0107 T, La\u0161karin G, Cupurdija K, \u0160trbo N, Koyama K, Okamura H, Podack ER, Rukavina D. IL\u201018 is present at the maternal\u2010fetal interface and enhances cytotoxic activity of decidual lymphocytes. AJRI 2002; 48:191\u2013200 \u00a9 Blackwell Munksgaard, 2002<\/jats:italic> <\/jats:p><jats:p>PROBLEM: Perforin expressing uterine natural killer (uNK) cells are under complex cytokine influence. The aim of the study was to investigate the presence and role of interleukin (IL)\u201018 on NK cytolytic potential at maternal\u2013fetal (M\u2013F) interface.<\/jats:p><jats:p>METHOD OF STUDY: Peripheral blood cells and decidual tissue were obtained from elective pregnancy termination of normal human 6\u201310\u2010week\u2010old pregnancies. Perforin expression and cytolytic activity of peripheral blood (PBL) and decidual lymphocytes (DL) were analyzed by flow cytometry. IL\u201018 positive decidual adherent cells (DAC) were detected by the same method. Interleukin\u201018 and IL\u201018 receptor (IL\u201018R) expression on the trophoblastic cells was detected by immunohistology using biotinylated anti\u2010IL\u201018 and IL\u201018R monoclonal antibodies.<\/jats:p><jats:p>RESULTS: The IL\u201018 added in a dose of 10\u2003ng\/mL up\u2010regulates perforin expression and cytolytic activity of DL. Simultaneous stimulation with IL\u201018 and IL\u201012 enhanced DL cytolytic activity, while IL\u201018 combined with IL\u201010 or IL\u201015 did not show this effect. Cytolytic activity of PBL was up\u2010regulated by IL\u201018 as well, and this effect was enhanced by the addition of IL\u201012 and IL\u201015. Interleukin\u201018 did not affect perforin\u2010protein expression in cultured PBL. Approximately 20% of DAC were IL\u201018 positive and these cells were mostly human leukocyte antigen (HLA)\u2010DR negative. IL\u201018R positive cells were found on syncytiotrophoblast cell layer, interstitial tissue cells of villi and fetal blood cells. There was no detectable IL\u201018 staining on trophoblast cell layer on villi, but strong staining of fetal blood cells in villous vessels.<\/jats:p><jats:p>CONCLUSION: These are first results showing IL\u201018R expression, but not IL\u201018 expression on villous trophoblastic cells, as well as enhancement of perforin expression and NK cytolytic potential of DL under the influence of IL\u201018. IL\u201018 in concert with other cytokines and hormones could play an important role in the regulation of cytolytic potential of first trimester pregnancy decidual and peripheral blood NK cells.<\/jats:p>","DOI":"10.1034\/j.1600-0897.2002.01132.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:26:59Z","timestamp":1047346019000},"page":"191-200","source":"Crossref","is-referenced-by-count":35,"title":["IL\u201018 is Present at the Maternal\u2013Fetal Interface and Enhances Cytotoxic Activity of Decidual Lymphocytes"],"prefix":"10.1111","volume":"48","author":[{"given":"VLATKA 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