{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T06:46:09Z","timestamp":1773211569367,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2003,1,15]],"date-time":"2003-01-15T00:00:00Z","timestamp":1042588800000},"content-version":"vor","delay-in-days":75,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:p>Canellada A, Blois S, Gentile T, Margni Idehu RA. <jats:italic>In vitro<\/jats:italic> modulation of protective antibody responses by estrogen, progesterone and interleukin\u20106. AJRI 2002; 48:334\u2013343 \u00a9 Blackwell Munksgaard, 2002<\/jats:p><jats:p>PROBLEM: We have previously demonstrated that the addition of placental interleukin\u20106 (IL\u20106) to murine hybridomas increased asymmetric antibody synthesis. Here we analyze whether progesterone (Pg) and estrogen (E<jats:sub>2<\/jats:sub>) affect asymmetric antibody synthesis by modulating IL\u20106 production in hybridoma cells.<\/jats:p><jats:p>METHOD OF STUDY: Hybridoma 112D5 B cells were cultured with E<jats:sub>2<\/jats:sub>, Pg or recombinant IL\u20106. Cell proliferation was assessed by <jats:sup>3<\/jats:sup>H\u2010thymidine incorporation, and asymmetric antibodies were measured in culture supernatants by Con A fixation and enzyme\u2010linked immunusorbant assay (ELISA). E<jats:sub>2<\/jats:sub> and Pg\u2010receptors (ER and PR) were evaluated in whole cell extracts by Western blot. IL\u20106 was measured in culture supernatants by ELISA.<\/jats:p><jats:p>RESULTS: 112D5 expressed both PR and ER, which were differentially regulated. At 48\u2003hr, Pg and E<jats:sub>2<\/jats:sub> slightly decreased cell proliferation whereas IL\u20106 did not. As well as IL\u20106, 10<jats:sup>\u201310<\/jats:sup>\u2003<jats:sc>M<\/jats:sc> Pg but not E<jats:sub>2<\/jats:sub> induced asymmetric antibody production. Interestingly, Pg at 10<jats:sup>\u20136<\/jats:sup>\u2003<jats:sc>M<\/jats:sc> decreased asymmetric antibody synthesis by hybridoma cells. Finally, mainly Pg but also E<jats:sub>2<\/jats:sub> increased IL\u20106 synthesis, although IL\u20106 levels did not correlate with asymmetric antibodies synthesized in the presence of E<jats:sub>2<\/jats:sub> or Pg.<\/jats:p><jats:p>CONCLUSIONS: In cells expressing both ER and PR, we could demonstrate that steroids participate in humoral immune responses by modulating asymmetric antibody synthesis. IL\u20106 proved to be only partially involved. Other possible mechanisms involved in the effect of Pg on blocking antibody responses and their contribution to a successful pregnancy are discussed in the paper.<\/jats:p>","DOI":"10.1034\/j.1600-0897.2002.01141.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:27:15Z","timestamp":1047346035000},"page":"334-343","source":"Crossref","is-referenced-by-count":55,"title":["<i>In Vitro<\/i> Modulation of Protective Antibody Responses by Estrogen, Progesterone and Interleukin\u20106"],"prefix":"10.1111","volume":"48","author":[{"given":"Andrea","family":"Canellada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Blois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Gentile","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo A.","family":"Margni Idehu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,1,15]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(01)00044-3"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3170329"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-4310(98)01303-3"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1994.1262"},{"key":"e_1_2_6_6_2","unstructured":"CosmaJ.InThymic Hormones Turck TD (ed.). Baltimore MD University Park Press 1973 pp 59\u201389."},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-69-6-1200"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(93)90080-G"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"128","DOI":"10.4049\/jimmunol.155.1.128","article-title":"Progesterone favors the development of human T helper cells producing Th2 type cytokines and promotes both IL\u20104 production and membrane CD30 expression in established Th1 cell clones","volume":"155","author":"Piccinni MP","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/4.1.77"},{"key":"e_1_2_6_11_2","first-page":"184","article-title":"Trophoblast derived IL\u20106 regulates hCG release through IL\u20106 receptor on human trophoblasts","volume":"74","author":"Nishino E","year":"1990","journal-title":"J Clin Endocrinol Metab"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6121"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1997.1090"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/324073a0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.5.1726"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.4.967"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.3.914"},{"key":"e_1_2_6_18_2","doi-asserted-by":"crossref","first-page":"813","DOI":"10.4049\/jimmunol.139.3.813","article-title":"Purification and NH2\u2010terminal amino acid sequence of a plasmacytoma growth factor derived from the murine macrophage cell line","volume":"139","author":"Nordan R","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(92)90290-6"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1996.tb00053.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1997.tb00313.x"},{"key":"e_1_2_6_22_2","first-page":"529","article-title":"Influence of mouse placental factors on in vitro antibody synthesis","volume":"35","author":"Miranda S","year":"1996","journal-title":"J Reprod Immunol"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.06.040188.002535"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1998.tb01189.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0378(91)90029-P"},{"key":"e_1_2_6_26_2","first-page":"153","article-title":"Role of asymmetric antibodies in fetal maintenance","volume":"2","author":"Margni RA","year":"1999","journal-title":"Curr Trends Immunol"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(00)00074-7"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1989.tb00565.x"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1995.tb00887.x"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1996.tb00153.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1089\/152581600319478"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(93)90081-L"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0378(00)00060-7"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.8755-8920.2000.440101.x"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1996.tb00041.x"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1999.tb00464.x"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1159\/000010315"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(99)00505-1"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1996.0016"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(88)90124-0"},{"key":"e_1_2_6_43_2","doi-asserted-by":"crossref","unstructured":"CanelladaA MargniR:Immunomodulatory action of dexamethasone on monoclonal antibody producing hybridoma cells.Hybridoma and hybridomics2002(in press).","DOI":"10.1089\/153685902760173935"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1330598"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.1999.1097"},{"key":"e_1_2_6_46_2","first-page":"1538","article-title":"Interleukin\u20101 alpha and interleukin\u20106 act additively to inhibit growth of MCF\u20107 breast cancer cells in vitro","volume":"53","author":"Danforth DN","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.139.8.3597"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1994.1262"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(94)90142-2"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1998.0394"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.29733.x"},{"key":"e_1_2_6_52_2","first-page":"6768","article-title":"Expression of gonadotropin receptor and growth responses to key reproductive hormones in normal and malignant human ovarian surface epithelial cells","volume":"61","author":"Syed V","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4644(20001101)79:2<334::AID-JCB160>3.0.CO;2-M"},{"key":"e_1_2_6_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.19.5627"},{"key":"e_1_2_6_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)67736-X"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0897.2001.d01-1.x"},{"key":"e_1_2_6_57_2","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.4049\/jimmunol.159.5.2327","article-title":"The glycosylation of IgA produced by murine B cells is altered by Th2 cytokines","volume":"159","author":"Chintalacharuvu SR","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_6_58_2","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.2000.0699"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240570405"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/14.12.2974"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.8755-8920.2000.440506.x"},{"key":"e_1_2_6_62_2","first-page":"371","article-title":"Interleukin regulation of asymmetric IgG synthesized by isolated placental B cells","volume":"45","author":"Canellada A","year":"2001","journal-title":"Am J Reprod Immunol"}],"container-title":["American Journal of Reproductive Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0897.2002.01141.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0897.2002.01141.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0897.2002.01141.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T08:06:32Z","timestamp":1697529992000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0897.2002.01141.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11]]},"references-count":61,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,11]]}},"alternative-id":["10.1034\/j.1600-0897.2002.01141.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0897.2002.01141.x","archive":["Portico"],"relation":{},"ISSN":["1046-7408","1600-0897"],"issn-type":[{"value":"1046-7408","type":"print"},{"value":"1600-0897","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11]]}}}