{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:06:28Z","timestamp":1791198388026,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,2,21]],"date-time":"2003-02-21T00:00:00Z","timestamp":1045785600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Rep Immunol"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:p><jats:bold>PROBLEM:\u2002<\/jats:bold> To determine if interleukin\u201016 (IL\u201016), IL\u201017, and IL\u201018 are present at the murine fetomaternal interface during pregnancy as a first step towards investigating their roles in fetomaternal relationship.<\/jats:p><jats:p><jats:bold>METHODS:\u2002<\/jats:bold> Expression of IL\u201016, IL\u201017, and IL\u201018, was assessed by immunohistochemistry (IHC) in the BALB\/c\u2003\u00d7\u2003BALB\/k (H2<jats:sup>d<\/jats:sup>\u2003\u00d7\u2003H2<jats:sup>k<\/jats:sup>), and the CBA\/J\u2003\u00d7\u2003BALB\/c non\u2010abortion prone, and CBA\/J\u2003\u00d7\u2003DBA\/2 abortion prone matings. Enzyme\u2010linked immunosorbent assay (ELISA) were performed for the two latter cytokines to compare local production in the abortion prone CBA\/J\u2003\u00d7\u2003DBA\/2 versus the non\u2010abortion prone CBA\/J\u2003\u00d7\u2003BALB\/c matings.<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> Expression of IL\u201017 was borderline. The anti\u2010IL\u201016 staining specifically localized in the uterine stroma and glandular epithelium and was rather low in the placenta. IL\u201018 staining started in the peri\u2010implantation uterus in the basal proliferative stroma, and was also traced, although weaker, in the glandular epithelium. In the immediate post\u2010implantation period, a weak stromal staining persisted but there was a strong labeling of the ectoplacental cone. Interestingly, when the ectoplacental cone differentiates into placenta having a major histocompatibility complex (MHC) class I+ spongiotrophoblast and a (MHC class I\u2013) labyrinth, a very strong transient labeling of uterine natural killer (u\u2010NK) cells was found. Later in gestation, IL\u201018 was also produced by giant cell and spongiotrophoblast. Finally, we compared by ELISA the production of IL\u201017\/\u201018 in CBA\/J\u2003\u00d7\u2003DBA\/2 and CBA\/J\u2003\u00d7\u2003BALB\/c matings. We detected significantly more IL\u201018 in the non\u2010abortion prone combination decidua or placenta.<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2002<\/jats:bold> The three cytokines IL\u201016, IL\u201017, and IL\u201018 were detected at the fetomaternal interface with a tissue specific, stage\u2010dependent distribution. The predominance of IL\u201018 secretion in the non\u2010resorption prone matings lead us to question the general validity of the classical T\u2010helper (Th)1\/2 paradigm.<\/jats:p>","DOI":"10.1034\/j.1600-0897.2003.01150.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:28:10Z","timestamp":1047346090000},"page":"101-112","source":"Crossref","is-referenced-by-count":57,"title":["Demonstration of the Presence of IL\u201016, IL\u201017 and IL\u201018 at the Murine Fetomaternal Interface during Murine Pregnancy"],"prefix":"10.1111","volume":"49","author":[{"given":"S.","family":"Ostojic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Dubanchet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Chaouat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Abdelkarim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Truyens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Capron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,2,21]]},"reference":[{"key":"e_1_2_19_2_2","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0889-8561(22)00249-1","article-title":"The role of lymphokines in pregnancy","volume":"10","author":"Toder V","year":"1990","journal-title":"Imunol Allerg Clin No Am"},{"key":"e_1_2_19_3_2","first-page":"49","article-title":"Lymphokines, cytokines and immunoregulators involved in pregnancy success or failure and perinatal AIDS","volume":"93","author":"Chaouat G","year":"1991","journal-title":"Period Biol"},{"key":"e_1_2_19_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(99)00457-4"},{"key":"e_1_2_19_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90235-D"},{"key":"e_1_2_19_6_2","doi-asserted-by":"crossref","first-page":"4562","DOI":"10.4049\/jimmunol.151.9.4562","article-title":"Synthesis of T helper\u20102 cytokines at the maternal fetal interface","volume":"151","author":"Lin H","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_19_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1997.tb00255.x"},{"key":"e_1_2_19_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1987.tb00156.x"},{"key":"e_1_2_19_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(97)01127-4"},{"key":"e_1_2_19_10_2","first-page":"93","article-title":"Murine experimental abortion by IL\u20102 administration is caused by activation of cytotoxic lymphocytes and placental apoptosis","volume":"48","author":"Shiraishi H","year":"1996","journal-title":"J Clin Lab Immunol"},{"key":"e_1_2_19_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.1999.tb00459.x"},{"key":"e_1_2_19_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01607.x"},{"key":"e_1_2_19_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.128.6.2563"},{"key":"e_1_2_19_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(96)10052-I"},{"key":"e_1_2_19_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.150.12.5445"},{"key":"e_1_2_19_16_2","doi-asserted-by":"publisher","DOI":"10.3109\/08830189809043008"},{"key":"e_1_2_19_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.57.2.590-595.1989"},{"key":"e_1_2_19_18_2","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1999.0501"},{"key":"e_1_2_19_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/378088a0"},{"key":"e_1_2_19_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260736"},{"key":"e_1_2_19_21_2","doi-asserted-by":"crossref","first-page":"3400","DOI":"10.4049\/jimmunol.161.7.3400","article-title":"IL\u201012 up\u2010regulates IL\u201018 receptor expression on T cells, Th1 cells, and B cells: synergism with IL\u201018 for IFN\u2010gamma production","volume":"161","author":"Yoshimoto T","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_19_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1379"},{"key":"e_1_2_19_23_2","doi-asserted-by":"crossref","first-page":"4261","DOI":"10.4049\/jimmunol.154.9.4261","article-title":"IL\u201010 prevents naturally occuring fetal loss in the CBA \u00d7 DBA\/2 mating combination, and local defect in IL\u201010 production in this abortion\u2010prone combination is corrected by in vivo injection of IFN\u2010tau","volume":"154","author":"Chaouat G","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_19_24_2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.4049\/jimmunol.156.2.653","article-title":"T\u2010helper 1 response against Leishmania major in pregnant C57Bl\/6 mice increases implantation failure and fetal resorptions. 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