{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T16:28:57Z","timestamp":1784564937227,"version":"3.55.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"6800","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2000,9]]},"DOI":"10.1038\/35024074","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T08:45:49Z","timestamp":1027673149000},"page":"81-86","source":"Crossref","is-referenced-by-count":1133,"title":["Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS"],"prefix":"10.1038","volume":"407","author":[{"given":"Shuji","family":"Shigenobu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemi","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahira","family":"Hattori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Sakaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"BF35024074_CR1","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1099\/00207713-41-4-566","volume":"41","author":"MA Munson","year":"1991","unstructured":"Munson, M. A., Baumann, P. & Kinsey, M. G. Buchnera, new genus and Buchnera aphidicola , new species, a taxon consisting of the mycetocyte-associated, primary endosymbionts of aphids. Int. J. Syst. Bacteriol. 41 , 566\u2013568 (1991).","journal-title":"Int. J. Syst. Bacteriol."},{"key":"BF35024074_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0074-7696(08)60637-3","volume":"116","author":"H Ishikawa","year":"1989","unstructured":"Ishikawa, H. Biochemical and molecular aspects of endosymbiosis in insects. Int. Rev. Cytol. 116, 1\u201345 (1989).","journal-title":"Int. Rev. Cytol."},{"key":"BF35024074_CR3","volume-title":"The Prokaryotes","author":"P Baumann","year":"2000","unstructured":"Baumann, P., Moran, N. A. & Baumann, L. in The Prokaryotes (ed. Dworkin, M.) (Springer, New York, 2000)."},{"key":"BF35024074_CR4","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/PL00006516","volume":"48","author":"K Komaki","year":"1999","unstructured":"Komaki, K. & Ishikawa, H. Intracellular bacterial symbionts of aphids possess many genomic copies per bacterium. J. Mol. Evol. 48, 717\u2013722 ( 1999).","journal-title":"J. Mol. Evol."},{"key":"BF35024074_CR5","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1007\/PL00006452","volume":"48","author":"H Charles","year":"1999","unstructured":"Charles, H. & Ishikawa, H. Physical and genetic map of the genome of Buchnera, the primary endosymbiont of the pea aphid Acyrthosiphon pisum. J. Mol. Evol. 48 142\u2013 150 (1999).","journal-title":"J. Mol. Evol."},{"key":"BF35024074_CR6","volume-title":"Endosymbiosis of Animals with Plant Microorganisms","author":"P Buchner","year":"1965","unstructured":"Buchner, P. Endosymbiosis of Animals with Plant Microorganisms (Wiley, New York, 1965)."},{"key":"BF35024074_CR7","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1098\/rspb.1993.0098","volume":"253","author":"NA Moran","year":"1993","unstructured":"Moran, N. A., Munson, M. A., Baumann, P. & Ishikawa, H. A molecular clock in endosymbiotic bacteria is calibrated using the insect hosts. Proc. R. Soc. Lond. B 253, 167\u2013 171 (1993).","journal-title":"Proc. R. Soc. Lond. B"},{"key":"BF35024074_CR8","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1126\/science.270.5235.397","volume":"270","author":"CM Fraser","year":"1995","unstructured":"Fraser, C. M. et al. The minimal gene complement of Mycoplasma genitalium. Science 270, 397\u2013403 (1995).","journal-title":"Science"},{"key":"BF35024074_CR9","doi-asserted-by":"publisher","first-page":"4768","DOI":"10.1128\/jb.179.15.4768-4777.1997","volume":"179","author":"RC van Ham","year":"1997","unstructured":"van Ham, R. C., Moya, A. & Latorre, A. Putative evolutionary origin of plasmids carrying the genes involved in leucine biosynthesis in Buchnera aphidicola (endosymbiont of aphids). J. Bacteriol. 179, 4768\u2013 4777 (1997).","journal-title":"J. Bacteriol."},{"key":"BF35024074_CR10","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1146\/annurev.ento.43.1.17","volume":"43","author":"AE Douglas","year":"1998","unstructured":"Douglas, A. E. Nutritional interactions in insect-microbial symbioses: aphids and their symbiotic bacteria Buchnera. Annu. Rev. Entomol. 43, 17\u201337 (1998).","journal-title":"Annu. Rev. Entomol."},{"key":"BF35024074_CR11","doi-asserted-by":"publisher","first-page":"2873","DOI":"10.1073\/pnas.93.7.2873","volume":"93","author":"NA Moran","year":"1996","unstructured":"Moran, N. A. Accelerated evolution and Muller's rachet in endosymbiotic bacteria. Proc. Natl Acad. Sci. USA 93, 2873\u2013 2878 (1996).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BF35024074_CR12","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1146\/annurev.en.25.010180.001113","volume":"25","author":"EJ Houk","year":"1980","unstructured":"Houk, E. J. & Griffiths, G. W. Intracellular symbiotes of the Homopltera. Annu. Rev. Entomol. 25, 161\u2013187 (1980).","journal-title":"Annu. Rev. Entomol."},{"key":"BF35024074_CR13","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/0022-1910(94)00080-Z","volume":"41","author":"T Sasaki","year":"1995","unstructured":"Sasaki, T. & Ishikawa, H. Production of essential amino acids from glutamate by mycetocyte symbionts of the pea aphid, Acyrthosiphon pisum. J. Insect. Physiol. 41, 41\u2013 46 (1995).","journal-title":"J. Insect. Physiol."},{"key":"BF35024074_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0022-1910(98)00104-8","volume":"45","author":"A Nakabachi","year":"1999","unstructured":"Nakabachi, A. & Ishikawa, H. Provision of riboflavin to the host aphid, Acyrothosiphon pisum, by endosymbiotic bacteria, Buchnera . J. Insect. Physiol. 45, 1\u2013 6 (1999).","journal-title":"J. Insect. Physiol."},{"key":"BF35024074_CR15","doi-asserted-by":"publisher","first-page":"1023","DOI":"10.1093\/jn\/101.8.1023","volume":"101","author":"TE Mittler","year":"1971","unstructured":"Mittler, T. E. Dietary amino acid requirements of Aphid Myzus persicae affected by antibiotic uptake. J.\u00a0Nutr. 101, 1023 \u20131028 (1971).","journal-title":"J.\u00a0Nutr."},{"key":"BF35024074_CR16","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1099\/00221287-139-4-821","volume":"139","author":"LF Whitehead","year":"1993","unstructured":"Whitehead, L. F. & Douglas, A. E. A metabolic study of Buchnera, the intracellular bacterial symbionts of the pea aphid Acyrthosiphon pisum. J. Gen. Microbiol. 139, 821\u2013826 (1993).","journal-title":"J. Gen. Microbiol."},{"key":"BF35024074_CR17","first-page":"599-605","volume":"18","author":"AE Douglas","year":"1988","unstructured":"Douglas, A. E. Sulphate utilization in an aphid symbiosis. Insect. Biochem. Mol. Biol. 18, 599-605 (1988).","journal-title":"Insect. Biochem. Mol. Biol."},{"key":"BF35024074_CR18","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1101\/gr.8.10.1048","volume":"8","author":"K Tomii","year":"1998","unstructured":"Tomii, K. & Kanehisa, M. A comparative analysis of ABC transporters in complete microbial genomes. Genome Res. 8, 1048\u20131059 (1998).","journal-title":"Genome Res."},{"key":"BF35024074_CR19","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1126\/science.280.5363.602","volume":"280","author":"T Kubori","year":"1998","unstructured":"Kubori, T. et al. Supramolecular structure of the Salmonella typhimurium type III protein secretion system. Science 280 , 602\u2013605 (1998).","journal-title":"Science"},{"key":"BF35024074_CR20","doi-asserted-by":"publisher","first-page":"6456","DOI":"10.1073\/pnas.96.11.6456","volume":"96","author":"GM Young","year":"1999","unstructured":"Young, G. M., Schmiel, D. H. & Miller, V. L. A new pathway for the secretion of virulence factors by bacteria: the flagellar export apparatus functions as a protein-secretion system. Proc. Natl Acad. Sci. USA 96, 6456 \u20136461 (1999).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BF35024074_CR21","doi-asserted-by":"publisher","first-page":"7003","DOI":"10.1128\/jb.178.23.7003-7009.1996","volume":"178","author":"A Marais","year":"1996","unstructured":"Marais, A., Bove, J. M. & Renaudin, J. Characterization of the recA gene regions of Spiroplasma citri and Spiroplasma melliferum. J. Bacteriol. 178, 7003\u20137009 ( 1996).","journal-title":"J. Bacteriol."},{"key":"BF35024074_CR22","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/S0921-8777(99)00050-6","volume":"435","author":"JA Eisen","year":"1999","unstructured":"Eisen, J. A. & Hanawalt, P. C. A phylogenomic study of DNA repair genes, proteins, and processes. Mutat. Res. 435, 171\u2013213 (1999).","journal-title":"Mutat. Res."},{"key":"BF35024074_CR23","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/S1369-5274(00)00068-0","volume":"3","author":"T Nogueira","year":"2000","unstructured":"Nogueira, T. & Springer, M. Post-transcriptional control by global regulators of gene expression in bacteria. Curr. Opin. Microbiol. 3, 154\u2013158 ( 2000).","journal-title":"Curr. Opin. Microbiol."},{"key":"BF35024074_CR24","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1126\/science.7542800","volume":"269","author":"RD Fleischmann","year":"1995","unstructured":"Fleischmann, R. D. et al. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269, 496\u2013 512 (1995).","journal-title":"Science"},{"key":"BF35024074_CR25","doi-asserted-by":"publisher","first-page":"1802","DOI":"10.1093\/nar\/25.9.1802","volume":"25","author":"M Hattori","year":"1997","unstructured":"Hattori, M. et al. A novel method for making nested deletions and its application for sequencing of a 300 kb region of human APP locus. Nucleic Acids Res. 25, 1802\u20131808 ( 1997).","journal-title":"Nucleic Acids Res."},{"key":"BF35024074_CR26","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1093\/dnares\/3.6.355","volume":"3","author":"T Yada","year":"1996","unstructured":"Yada, T. & Hirosawa, M. Detection of short protein coding regions within the cyanobacterium genome: application of the hidden Markov model. DNA Res. 3, 355\u2013 361 (1996).","journal-title":"DNA Res."},{"key":"BF35024074_CR27","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/41483","volume":"388","author":"JF Tomb","year":"1997","unstructured":"Tomb, J. F. et al. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388, 539\u2013 547 (1997).","journal-title":"Nature"},{"key":"BF35024074_CR28","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1093\/nar\/27.1.29","volume":"27","author":"H Ogata","year":"1999","unstructured":"Ogata, H. et al. KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 27, 29\u201334 ( 1999).","journal-title":"Nucleic Acids Res."},{"key":"BF35024074_CR29","doi-asserted-by":"publisher","first-page":"S57","DOI":"10.1007\/PL00000052","volume":"44","author":"H Watanabe","year":"1997","unstructured":"Watanabe, H., Mori, H., Itoh, T. & Gojobori, T. Genome plasticity as a paradigm of eubacteria evolution. J. Mol. Evol. 44 (Suppl. 1), S57\u2013S64 ( 1997).","journal-title":"J. Mol. Evol."},{"key":"BF35024074_CR30","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1128\/MR.57.4.862-952.1993","volume":"57","author":"M Riley","year":"1993","unstructured":"Riley, M. Functions of the gene products of Escherichia coli. Microbiol. Rev. 57, 862\u2013952 ( 1993).","journal-title":"Microbiol. Rev."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/35024074.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/35024074","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/35024074.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T02:06:21Z","timestamp":1684289181000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/35024074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":30,"journal-issue":{"issue":"6800","published-print":{"date-parts":[[2000,9]]}},"alternative-id":["BF35024074"],"URL":"https:\/\/doi.org\/10.1038\/35024074","relation":{},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9]]}}}