{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:53:47Z","timestamp":1791410027692,"version":"4.3.3"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"6634","license":[{"start":{"date-parts":[[1997,6,12]],"date-time":"1997-06-12T00:00:00Z","timestamp":866073600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[1997,6,12]],"date-time":"1997-06-12T00:00:00Z","timestamp":866073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[1997,6,12]]},"DOI":"10.1038\/42743","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T08:35:11Z","timestamp":1027672511000},"page":"729-733","source":"Crossref","is-referenced-by-count":463,"title":["Toxin-induced activation of the G protein p21\u2009Rho by deamidation of glutamine"],"prefix":"10.1038","volume":"387","author":[{"given":"Gilles","family":"Flatau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Lemichez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Gauthier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Chardin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Paris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Fiorentini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Boquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"BF42743_CR1","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1128\/iai.39.3.1300-1306.1983","volume":"39","author":"A Caprioli","year":"1983","unstructured":"Caprioli, A., Falbo, V., Roda, L. G., Ruggeri, F. M. & Zona, C. Partial purification and characterization of an Escherichia coli toxic factor that induces morphological alterations. Infect. Immun. 39, 1300\u20131306 (1983).","journal-title":"Infect. Immun."},{"key":"BF42743_CR2","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1128\/jcm.28.4.694-699.1990","volume":"28","author":"J De Rycke","year":"1990","unstructured":"De Rycke, J.et al. Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates. J. Clin. Microbiol. 28, 694\u2013699 (1990).","journal-title":"J. Clin. Microbiol."},{"key":"BF42743_CR3","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1016\/0041-0101(88)90203-6","volume":"26","author":"C Fiorentini","year":"1988","unstructured":"Fiorentini, C.et al. Cytoskeletal changes induced in HEp-2 cells by the cytotoxic necrotizing factor of Escherichia coli. Toxicon 26, 1047\u20131056 (1988).","journal-title":"Toxicon"},{"key":"BF42743_CR4","doi-asserted-by":"publisher","first-page":"3814","DOI":"10.1073\/pnas.91.9.3814","volume":"91","author":"E Oswald","year":"1994","unstructured":"Oswald, E.et al. Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin stress fibers. Proc. Natl Acad. Sci. USA 91, 3814\u20133818 (1994).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BF42743_CR5","first-page":"404","volume":"24","author":"C Fiorentini","year":"1994","unstructured":"Fiorentini, C.et al. Escherichia coli cytotoxic necrotizing factor 1 increases actin assembly via the p21 Rho protein. P. Zbl. Bakt. (suppl.) 24, 404\u2013405 (1994).","journal-title":"P. Zbl. Bakt. (suppl.)"},{"key":"BF42743_CR6","doi-asserted-by":"crossref","first-page":"3936","DOI":"10.1128\/iai.63.10.3936-3944.1995","volume":"63","author":"C Fiorentini","year":"1995","unstructured":"Fiorentini, C.et al. Escherichia coli cytotoxic necrotizing factor 1: evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase. Infect. Immun. 63, 3936\u20133944 (1995).","journal-title":"Infect. Immun."},{"key":"BF42743_CR7","doi-asserted-by":"publisher","first-page":"1869","DOI":"10.1093\/nar\/15.4.1869","volume":"15","author":"P Yeramian","year":"1987","unstructured":"Yeramian, P., Chardin, P., Madaule, P. & Tavitian, A. Nucleotide sequence of human Rho cDNA clone 12. Nucleic Acids Res. 15, 1869 (1987).","journal-title":"Nucleic Acids Res."},{"key":"BF42743_CR8","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/0092-8674(86)90495-2","volume":"44","author":"CJ Der","year":"1986","unstructured":"Der, C. J., Finkel, T. & Cooper, G. M. Biological and biochemical properties of human ras H genes mutated at codon 61. Cell 44, 167\u2013176 (1986).","journal-title":"Cell"},{"key":"BF42743_CR9","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1073\/pnas.82.1.38","volume":"82","author":"SK Srivastava","year":"1985","unstructured":"Srivastava, S. K., Yuasa, Y., Reynolds, S. H. & Aaronson, S. A. Effects of two major activating lesions on the structure and conformation of human Ras oncogene products. Proc. Natl Acad. Sci. USA 82, 38\u201342 (1985).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BF42743_CR10","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1042\/bj2760833","volume":"276","author":"MD Garrett","year":"1991","unstructured":"Garrett, M. D., Major, G. N., Totty, N. & Hall, A. Purification and N-terminal sequence of the p21 Rho GTPase activating protein RhoGAP. Biochem. J. 276, 833\u2013836 (1991).","journal-title":"Biochem. J."},{"key":"BF42743_CR11","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/0076-6879(95)56036-X","volume":"256","author":"AJ Ridley","year":"1995","unstructured":"Ridley, A. J. Microinjection of Rho and Rac into quiescent swiss 3T3 cells. Methods Enzymol. 256, 313\u2013320 (1995).","journal-title":"Methods Enzymol."},{"key":"BF42743_CR12","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1016\/0962-8924(96)10026-X","volume":"6","author":"LM Machesky","year":"1996","unstructured":"Machesky, L. M. & Hall, A. Rho: a connection between membrane receptor signalling and the cytoskeleton. Trends Cell Biol. 6, 304\u2013310 (1996).","journal-title":"Trends Cell Biol."},{"key":"BF42743_CR13","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/0092-8674(92)90163-7","volume":"70","author":"AJ Ridley","year":"1992","unstructured":"Ridley, A. J. & Hall, A. The small GTP-binding protein Rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70, 389\u2013400 (1992).","journal-title":"Cell"},{"key":"BF42743_CR14","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1016\/0092-8674(92)90164-8","volume":"70","author":"AJ Ridley","year":"1992","unstructured":"Ridley, A. J., Paterson, H. F., Johnston, C. L., Diekmann, D. & Hall, A. The small GTP-binding protein Rac regulates growth factor-induced membrane ruffling. Cell 70, 401\u2013410 (1992).","journal-title":"Cell"},{"key":"BF42743_CR15","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/0092-8674(95)90370-4","volume":"81","author":"CD Nobes","year":"1995","unstructured":"Nobes, C. D. & Hall, A. Rho, Rac and Cdc42 GTPases regulate the assembly of multimolecular complexes associated with actin stress fibers, lamellipodia and filopodia. Cell 81, 53\u201362 (1995).","journal-title":"Cell"},{"key":"BF42743_CR16","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1111\/j.1365-2958.1993.tb01254.x","volume":"9","author":"L Falzano","year":"1993","unstructured":"Falzano, L.et al. Induction of phagocytic behaviour in human epithelial cells by Escherichia coli cytotoxic necrotizing factor type 1. Mol. Microbiol. 9, 1247\u20131254 (1993).","journal-title":"Mol. Microbiol."},{"key":"BF42743_CR17","doi-asserted-by":"publisher","first-page":"2447","DOI":"10.1128\/MCB.14.4.2447","volume":"14","author":"T Nishiyama","year":"1994","unstructured":"Nishiyama, T.et al. Rac p21 is involved in insulin-induced membrane ruffling and Rho p21 is involved in hepatocyte growth factor-and 12-O-tetradecanoylphorbol-13 acetate (TPA)-induced membrane ruffling in KB cells. Mol. Cell. Biol. 14, 2447\u20132456 (1994).","journal-title":"Mol. Cell. Biol."},{"key":"BF42743_CR18","doi-asserted-by":"publisher","first-page":"3315","DOI":"10.1002\/j.1460-2075.1996.tb00696.x","volume":"15","author":"T Adam","year":"1996","unstructured":"Adam, T., Giry, M., Boquet, P. & Sansonetti, P. Rho-dependent membrane folding causes Shigella entry into cells. EMBO J. 15, 3315\u20133321 (1996).","journal-title":"EMBO J."},{"key":"BF42743_CR19","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1084\/jem.185.2.281","volume":"185","author":"M Watarai","year":"1997","unstructured":"Watarai, M., Kamata, Y., Kozaki, S. & Sasakawa, C. Rho, a small GTP-binding protein, is essential for Shigella invasion of epithelial cells. J. Exp. Med. 185, 281\u2013292 (1997).","journal-title":"J. Exp. Med."},{"key":"BF42743_CR20","doi-asserted-by":"crossref","first-page":"3817","DOI":"10.1128\/iai.62.9.3817-3828.1994","volume":"62","author":"KE Walker","year":"1994","unstructured":"Walker, K. E. & Weiss, A. Characterization of the dermonecrotic toxin in members of the genus Bordetella. Infect. Immun. 62, 3817\u20133828 (1994).","journal-title":"Infect. Immun."},{"key":"BF42743_CR21","doi-asserted-by":"crossref","first-page":"4909","DOI":"10.1128\/iai.61.11.4909-4914.1993","volume":"61","author":"V Falbo","year":"1993","unstructured":"Falbo, V., Pace, T., Picci, L., Pizzi, E. & Caprioli, A. Isolation and nucleotide sequence of the gene encoding cytotoxic necrotizing factor 1 of Escherichia coli. Infect. Immun. 61, 4909\u20134914 (1993).","journal-title":"Infect. Immun."},{"key":"BF42743_CR22","doi-asserted-by":"crossref","first-page":"3243","DOI":"10.1242\/jcs.108.10.3243","volume":"108","author":"Y Horiguchi","year":"1995","unstructured":"Horiguchi, Y., Senda, T., Sugimoto, N., Katahira, J. & Matsuda, M. Bordetella bronchiseptica dermonecrotizing toxin stimulates assembly of actin stress fibers and focal adhesion points by modifying the small GTP-binding protein Rho. J. Cell Sci. 108, 3243\u20133251 (1995).","journal-title":"J. Cell Sci."},{"key":"BF42743_CR23","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/0076-6879(95)56003-3","volume":"256","author":"AJ Self","year":"1995","unstructured":"Self, A. J. & Hall, A. Purification of recombinant Rho\/Rac\/G25K from Escherichia coli. Methods Enzymol. 256, 3\u201310 (1995).","journal-title":"Methods Enzymol."},{"key":"BF42743_CR24","doi-asserted-by":"publisher","first-page":"1573","DOI":"10.1074\/jbc.271.3.1573","volume":"271","author":"M Franco","year":"1996","unstructured":"Franco, M., Chardin, P., Chabre, M. & Paris, S. Myristoylation of ADP-ribosylation factor 1 facilitates nucleotide exchange at physiological Mg2+\u2009levels. J. Biol. Chem. 271, 1573\u20131578 (1996).","journal-title":"J. Biol. Chem."},{"key":"BF42743_CR25","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/0076-6879(95)56010-6","volume":"256","author":"AJ Self","year":"1995","unstructured":"Self, A. J. & Hall, A. Measurement of intrinsic nucleotide exchange and GTP hydrolysis rates. Methods Enzymol. 256, 67\u201376 (1995).","journal-title":"Methods Enzymol."},{"key":"BF42743_CR26","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S0021-9258(19)75850-5","volume":"262","author":"T Higashijima","year":"1987","unstructured":"Higashijima, T., Ferguson, K. M., Smigel, M. D. & Gilman, A. G. The effect of GTP and Mg2+on the GTPase activity and the fluorescent properties of Go. J. Biol. Chem. 262, 757\u2013761 (1987).","journal-title":"J. Biol. Chem."},{"key":"BF42743_CR27","doi-asserted-by":"publisher","first-page":"1087","DOI":"10.1002\/j.1460-2075.1989.tb03477.x","volume":"8","author":"P Chardin","year":"1989","unstructured":"Chardin, P.et al. The mammalian G-protein RhoC is ADP-ribosylated by Clostridium botulinum C3 and affects actin microfilaments in Vero cells. EMBO J. 8, 1087\u20131092 (1989).","journal-title":"EMBO J."}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/42743.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/42743","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/doifinder\/10.1038\/42743","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/www.nature.com\/articles\/42743.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T18:17:38Z","timestamp":1708971458000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/42743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,12]]},"references-count":27,"journal-issue":{"issue":"6634","published-print":{"date-parts":[[1997,6,12]]}},"alternative-id":["BF42743"],"URL":"https:\/\/doi.org\/10.1038\/42743","relation":{},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,6,12]]}}}