{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:17:58Z","timestamp":1773289078385,"version":"3.50.1"},"reference-count":7,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Biotechnol"],"published-print":{"date-parts":[[2000,7]]},"DOI":"10.1038\/77363","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T08:35:11Z","timestamp":1027672511000},"page":"789-791","source":"Crossref","is-referenced-by-count":17,"title":["Seamless gene engineering using RNA- and DNA-overhang cloning"],"prefix":"10.1038","volume":"18","author":[{"given":"Vincent W.","family":"Coljee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather L.","family":"Murray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William F.","family":"Donahue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin A.","family":"Jarrell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"BFnbt0700_789_CR1","doi-asserted-by":"publisher","first-page":"1350","DOI":"10.1126\/science.2999980","volume":"230","author":"RK Saiki","year":"1985","unstructured":"Saiki, R.K. et al. Enzymatic amplification of \u03b2-Globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230, 1350\u20131354 (1985).","journal-title":"Science"},{"key":"BFnbt0700_789_CR2","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/S0065-3233(08)60367-8","volume":"48","author":"FB Perler","year":"1996","unstructured":"Perler, F.B., Kumar, S. & Kong, H. Thermostable DNA polymerases. Adv. Protein Chem. 48, 377\u2013435 (1996).","journal-title":"Adv. Protein Chem."},{"key":"BFnbt0700_789_CR3","doi-asserted-by":"publisher","first-page":"7661","DOI":"10.1021\/bi00245a001","volume":"30","author":"TW Myers","year":"1991","unstructured":"Myers, T.W. & Gelfand, D.H. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30, 7661\u20137666 (1991).","journal-title":"Biochemistry"},{"key":"BFnbt0700_789_CR4","doi-asserted-by":"publisher","first-page":"3546","DOI":"10.1093\/nar\/24.18.3546","volume":"24","author":"J Cline","year":"1996","unstructured":"Cline, J., Braman, J. C. & Hogrefe, H.H. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases. Nucleic Acids Res. 24, 3546\u20133551 (1996).","journal-title":"Nucleic Acids Res"},{"key":"BFnbt0700_789_CR5","doi-asserted-by":"publisher","first-page":"993","DOI":"10.1016\/0092-8674(84)90049-7","volume":"36","author":"AR Krainer","year":"1984","unstructured":"Krainer, A.R., Maniatis, T., Ruskin, B. & Green, M.R. Normal and mutant human \u03b2-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell 36, 993\u20131005 (1984).","journal-title":"Cell"},{"key":"BFnbt0700_789_CR6","doi-asserted-by":"publisher","first-page":"1580","DOI":"10.1126\/science.1699275","volume":"249","author":"B Sommer","year":"1990","unstructured":"Sommer, B. et al. Flip and flop: a cell-specific functional switch in glutamate-operated channels of the CNS. Science 249, 1580\u20131585 (1990).","journal-title":"Science"},{"key":"BFnbt0700_789_CR7","first-page":"657","volume":"9","author":"DA Mead","year":"1991","unstructured":"Mead, D.A., Kristy Pey, N., Herrnstadt, C., Marcil, R.A. & Smith, L.M. A universal method for the direct cloning of PCR amplified nucleic acid. Biotechnology (NY) 9, 657\u2013663 (1991).","journal-title":"Biotechnology (NY)"}],"container-title":["Nature Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nbt0700_789.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nbt0700_789","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nbt0700_789.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T20:02:03Z","timestamp":1684440123000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nbt0700_789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,7]]},"references-count":7,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2000,7]]}},"alternative-id":["BFnbt0700_789"],"URL":"https:\/\/doi.org\/10.1038\/77363","relation":{},"ISSN":["1087-0156","1546-1696"],"issn-type":[{"value":"1087-0156","type":"print"},{"value":"1546-1696","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,7]]}}}