{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T12:08:08Z","timestamp":1769429288231,"version":"3.49.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"11","license":[{"start":{"date-parts":[[2008,10,3]],"date-time":"2008-10-03T00:00:00Z","timestamp":1222992000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["www.embopress.org"],"crossmark-restriction":false},"short-container-title":["EMBO Reports"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:p>\n                    Cleavage of the amyloid precursor protein (APP) is a crucial event in Alzheimer disease pathogenesis that creates the amyloid\u2010\u03b2 peptide (A\u03b2) and liberates the carboxy\u2010terminal APP intracellular domain (AICD) into the cytosol. The interaction of the APP C terminus with the adaptor protein Fe65 mediates APP trafficking and signalling, and is thought to regulate APP processing and A\u03b2 generation. We determined the crystal structure of the AICD in complex with the C\u2010terminal phosphotyrosine\u2010binding (PTB) domain of Fe65. The unique interface involves the NPxY PTB\u2010binding motif and two \u03b1 helices. The amino\u2010terminal helix of the AICD is capped by threonine T\n                    <jats:sup>668<\/jats:sup>\n                    , an Alzheimer disease\u2010relevant phosphorylation site involved in Fe65\u2010binding regulation. The structure together with mutational studies, isothermal titration calorimetry and nuclear magnetic resonance experiments sets the stage for understanding T\n                    <jats:sup>668<\/jats:sup>\n                    phosphorylation\u2010dependent complex regulation at a molecular level. A molecular switch model is proposed.\n                  <\/jats:p>","DOI":"10.1038\/embor.2008.188","type":"journal-article","created":{"date-parts":[[2008,10,3]],"date-time":"2008-10-03T05:22:02Z","timestamp":1223011322000},"page":"1134-1140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Structure of the intracellular domain of the amyloid precursor protein in complex with Fe65\u2010PTB2"],"prefix":"10.1038","volume":"9","author":[{"given":"Jens","family":"Radzimanowski","sequence":"first","affiliation":[{"name":"Heidelberg University Biochemistry Center, University of Heidelberg, INF328  D\u201069120 Heidelberg Germany"}]},{"given":"Bernd","family":"Simon","sequence":"additional","affiliation":[{"name":"Institute of Structural Biology, Helmholtz Zentrum M\u00fcnchen, Ingolst\u00e4dter Landstrasse 1  D\u201085764 Neuherberg Germany"}]},{"given":"Michael","family":"Sattler","sequence":"additional","affiliation":[{"name":"Center of Molecular Biology, University of Heidelberg, INF282  D\u201069120 Heidelberg Germany"}]},{"given":"Konrad","family":"Beyreuther","sequence":"additional","affiliation":[{"name":"European Molecular Biology Laboratory, Meyerhofstrasse 1  D\u201069117 Heidelberg Germany"}]},{"given":"Irmgard","family":"Sinning","sequence":"additional","affiliation":[{"name":"Heidelberg University Biochemistry Center, University of Heidelberg, INF328  D\u201069120 Heidelberg Germany"}]},{"given":"Klemens","family":"Wild","sequence":"additional","affiliation":[{"name":"Heidelberg University Biochemistry Center, University of Heidelberg, INF328  D\u201069120 Heidelberg 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