{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T15:32:58Z","timestamp":1779204778733,"version":"3.51.4"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"7016","license":[{"start":{"date-parts":[[2004,11,1]],"date-time":"2004-11-01T00:00:00Z","timestamp":1099267200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2004,11]]},"DOI":"10.1038\/nature03035","type":"journal-article","created":{"date-parts":[[2004,11,24]],"date-time":"2004-11-24T13:19:42Z","timestamp":1101302382000},"page":"522-525","source":"Crossref","is-referenced-by-count":423,"title":["Human 5\u2032 \u2192 3\u2032 exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites"],"prefix":"10.1038","volume":"432","author":[{"given":"Steven","family":"West","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Gromak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nick J.","family":"Proudfoot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"BFnature03035_CR1","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/0968-0004(89)90132-1","volume":"14","author":"NJ Proudfoot","year":"1989","unstructured":"Proudfoot, N. J. How RNA polymerase II terminates transcription in higher eukaryotes. Trends Biochem. Sci. 14, 105\u2013110 (1989)","journal-title":"Trends Biochem. Sci."},{"key":"BFnature03035_CR2","doi-asserted-by":"publisher","first-page":"8306","DOI":"10.1073\/pnas.84.23.8306","volume":"84","author":"J Logan","year":"1987","unstructured":"Logan, J., Falck-Pedersen, E., Darnell, J. E. Jr & Shenk, T. A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene. Proc. Natl Acad. Sci. USA 84, 8306\u20138310 (1987)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature03035_CR3","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1016\/j.ceb.2004.03.007","volume":"16","author":"N Proudfoot","year":"2004","unstructured":"Proudfoot, N. New perspectives on connecting messenger RNA 3\u2032 end formation to transcription. Curr. Opin. Cell Biol. 16, 272\u2013278 (2004)","journal-title":"Curr. Opin. Cell Biol."},{"key":"BFnature03035_CR4","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1101\/gad.2.4.440","volume":"2","author":"S Connelly","year":"1988","unstructured":"Connelly, S. & Manley, J. L. A functional mRNA polyadenylation signal is required for transcription termination by RNA polymerase II. Genes Dev. 2, 440\u2013452 (1988)","journal-title":"Genes Dev."},{"key":"BFnature03035_CR5","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1016\/S1097-2765(00)80465-7","volume":"3","author":"YN Osheim","year":"1999","unstructured":"Osheim, Y. N., Proudfoot, N. J. & Beyer, A. L. EM visualization of transcription by RNA polymerase II: downstream termination requires a poly(A) signal but not transcript cleavage. Mol. Cell 3, 379\u2013387 (1999)","journal-title":"Mol. Cell"},{"key":"BFnature03035_CR6","doi-asserted-by":"publisher","first-page":"2759","DOI":"10.1101\/gad.12.17.2759","volume":"12","author":"G Bauren","year":"1998","unstructured":"Bauren, G., Belikov, S. & Wieslander, L. Transcriptional termination in the Balbiani ring 1 gene is closely coupled to 3\u2032-end formation and excision of the 3\u2032-terminal intron. Genes Dev. 12, 2759\u20132769 (1998)","journal-title":"Genes Dev."},{"key":"BFnature03035_CR7","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/S1097-2765(00)80464-5","volume":"3","author":"MJ Dye","year":"1999","unstructured":"Dye, M. J. & Proudfoot, N. J. Terminal exon definition occurs cotranscriptionally and promotes termination of RNA polymerase II. Mol. Cell 3, 371\u2013378 (1999)","journal-title":"Mol. Cell"},{"key":"BFnature03035_CR8","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1016\/S0092-8674(01)00372-5","volume":"105","author":"MJ Dye","year":"2001","unstructured":"Dye, M. J. & Proudfoot, N. J. Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II. Cell 105, 669\u2013681 (2001)","journal-title":"Cell"},{"key":"BFnature03035_CR9","doi-asserted-by":"crossref","unstructured":"Teixeira, A. et al. Auto-catalytic RNA cleavage in the human \u03b2-globin pre-mRNA promotes transcription termination. Nature doi:10.1038\/nature03032 (this issue)","DOI":"10.1038\/nature03032"},{"key":"BFnature03035_CR10","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/S0168-9525(02)00010-0","volume":"19","author":"H Cerutti","year":"2003","unstructured":"Cerutti, H. RNA interference: travelling in the cell and gaining functions. Trends Genet. 19, 39\u201346 (2003)","journal-title":"Trends Genet."},{"key":"BFnature03035_CR11","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1006\/geno.1999.5866","volume":"59","author":"M Zhang","year":"1999","unstructured":"Zhang, M. et al. Cloning and mapping of the XRN2 gene to human chromosome 20p11.1\u2013p11.2. Genomics 59, 252\u2013254 (1999)","journal-title":"Genomics"},{"key":"BFnature03035_CR12","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1016\/S1097-2765(03)00349-6","volume":"12","author":"F Lejeune","year":"2003","unstructured":"Lejeune, F., Li, X. & Maquat, L. E. Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities. Mol. Cell 12, 675\u2013687 (2003)","journal-title":"Mol. Cell"},{"key":"BFnature03035_CR13","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1101\/gad.6.7.1173","volume":"6","author":"DC Amberg","year":"1992","unstructured":"Amberg, D. C., Goldstein, A. L. & Cole, C. N. Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA. Genes Dev. 6, 1173\u20131189 (1992)","journal-title":"Genes Dev."},{"key":"BFnature03035_CR14","doi-asserted-by":"publisher","first-page":"2452","DOI":"10.1002\/j.1460-2075.1994.tb06530.x","volume":"13","author":"Y Henry","year":"1994","unstructured":"Henry, Y. et al. The 5\u2032 end of yeast 5.8S rRNA is generated by exonucleases from an upstream cleavage site. EMBO J. 13, 2452\u20132463 (1994)","journal-title":"EMBO J."},{"key":"BFnature03035_CR15","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1128\/MCB.18.3.1181","volume":"18","author":"E Petfalski","year":"1998","unstructured":"Petfalski, E., Dandekar, T., Henry, Y. & Tollervey, D. Processing of the precursors to small nucleolar RNAs and rRNAs requires common components. Mol. Cell. Biol. 18, 1181\u20131189 (1998)","journal-title":"Mol. Cell. Biol."},{"key":"BFnature03035_CR16","doi-asserted-by":"publisher","first-page":"16899","DOI":"10.1073\/pnas.242603899","volume":"99","author":"RL Strausberg","year":"2002","unstructured":"Strausberg, R. L. et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc. Natl Acad. Sci. USA 99, 16899\u201316903 (2002)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature03035_CR17","doi-asserted-by":"publisher","first-page":"6609","DOI":"10.1073\/pnas.96.12.6609","volume":"96","author":"DA Samarsky","year":"1999","unstructured":"Samarsky, D. A. et al. A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency. Proc. Natl Acad. Sci. USA 96, 6609\u20136614 (1999)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature03035_CR18","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1016\/j.molcel.2003.08.010","volume":"12","author":"P Huertas","year":"2003","unstructured":"Huertas, P. & Aguilera, A. Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination. Mol. Cell 12, 711\u2013721 (2003)","journal-title":"Mol. Cell"},{"key":"BFnature03035_CR19","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1002\/1439-7633(20020802)3:8<690::AID-CBIC690>3.0.CO;2-C","volume":"3","author":"C Hammann","year":"2002","unstructured":"Hammann, C. & Lilley, D. M. Folding and activity of the hammerhead ribozyme. Chembiochem 3, 690\u2013700 (2002)","journal-title":"Chembiochem"},{"key":"BFnature03035_CR20","doi-asserted-by":"publisher","first-page":"16063","DOI":"10.1074\/jbc.270.27.16063","volume":"270","author":"A Stevens","year":"1995","unstructured":"Stevens, A. & Poole, T. L. 5\u2032 exonuclease 2 of Saccharomyces cerevisiae. J. Biol. Chem. 270, 16063\u201316069 (1995)","journal-title":"J. Biol. Chem."},{"key":"BFnature03035_CR21","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1016\/S1097-2765(02)00544-0","volume":"9","author":"C Torchet","year":"2002","unstructured":"Torchet, C. et al. Processing of 3\u2032-extended read-through transcripts by the exosome can generate functional mRNAs. Mol. Cell 9, 1285\u20131296 (2002)","journal-title":"Mol. Cell"},{"key":"BFnature03035_CR22","doi-asserted-by":"crossref","unstructured":"Kim, M. et al. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II. Nature doi:10.1038\/nature03041 (this issue)","DOI":"10.1038\/nature03041"},{"key":"BFnature03035_CR23","doi-asserted-by":"publisher","first-page":"4287","DOI":"10.1093\/nar\/16.10.4287","volume":"16","author":"SE Adams","year":"1988","unstructured":"Adams, S. E. et al. Synthesis of a gene for the HIV transactivator protein TAT by a novel single-stranded approach involving in vivo gap repair. Nucleic Acids Res. 16, 4287\u20134298 (1988)","journal-title":"Nucleic Acids Res."},{"key":"BFnature03035_CR24","doi-asserted-by":"publisher","first-page":"2494","DOI":"10.1101\/gad.11.19.2494","volume":"11","author":"HL Ashe","year":"1997","unstructured":"Ashe, H. L., Monks, J., Wijgerde, M., Fraser, P. & Proudfoot, N. J. Intergenic transcription and transinduction of the human \u03b2-globin locus. Genes Dev. 11, 2494\u20132509 (1997)","journal-title":"Genes Dev."},{"key":"BFnature03035_CR25","doi-asserted-by":"publisher","first-page":"943","DOI":"10.1016\/S1097-2765(02)00645-7","volume":"10","author":"EJ Wagner","year":"2002","unstructured":"Wagner, E. J. & Garcia-Blanco, M. A. RNAi-mediated PTB depletion leads to enhanced exon definition. Mol. Cell 10, 943\u2013949 (2002)","journal-title":"Mol. Cell"},{"key":"BFnature03035_CR26","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S1097-2765(03)00502-1","volume":"13","author":"MC Wollerton","year":"2004","unstructured":"Wollerton, M. C., Gooding, C., Wagner, E. J., Garcia-Blanco, M. A. & Smith, C. W. Autoregulation of polypyrimidine tract binding protein by alternative splicing leading to nonsense-mediated decay. Mol. Cell 13, 91\u2013100 (2004)","journal-title":"Mol. Cell"},{"key":"BFnature03035_CR27","doi-asserted-by":"publisher","first-page":"8185","DOI":"10.1073\/pnas.122246099","volume":"99","author":"S Kadener","year":"2002","unstructured":"Kadener, S., Fededa, J. P., Rosbash, M. & Kornblihtt, A. R. Regulation of alternative splicing by a transcriptional enhancer through RNA pol\u2009II elongation. Proc. Natl Acad. Sci. USA 99, 8185\u20138190 (2002)","journal-title":"Proc. Natl Acad. Sci. USA"}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nature03035.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature03035","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature03035.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T14:27:56Z","timestamp":1684420076000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature03035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11]]},"references-count":27,"journal-issue":{"issue":"7016","published-print":{"date-parts":[[2004,11]]}},"alternative-id":["BFnature03035"],"URL":"https:\/\/doi.org\/10.1038\/nature03035","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1017496.259620","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1017496.263691","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11]]}}}