{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T14:43:59Z","timestamp":1779893039999,"version":"3.53.1"},"reference-count":41,"publisher":"Springer Science and Business Media LLC","issue":"7224","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2008,12]]},"DOI":"10.1038\/nature07666","type":"journal-article","created":{"date-parts":[[2008,12,17]],"date-time":"2008-12-17T14:41:10Z","timestamp":1229524870000},"page":"921-926","source":"Crossref","is-referenced-by-count":507,"title":["Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex"],"prefix":"10.1038","volume":"456","author":[{"given":"Yanli","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Juranek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Tuschl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinshaw J.","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","reference":[{"key":"BFnature07666_CR1","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1186\/gb-2008-9-2-210","volume":"9","author":"J Hock","year":"2008","unstructured":"Hock, J. & Meister, G. The Argonaute protein family. Genome Biol. 9, 210 (2008)","journal-title":"Genome Biol."},{"key":"BFnature07666_CR2","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.cell.2005.06.023","volume":"122","author":"W Filipowicz","year":"2005","unstructured":"Filipowicz, W. The nuts and bolts of the RISC machine. Cell 122, 17\u201320 (2005)","journal-title":"Cell"},{"key":"BFnature07666_CR3","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1038\/nrm2321","volume":"9","author":"G Hutvagner","year":"2008","unstructured":"Hutvagner, G. & Simard, M. J. Argonaute proteins: key players in RNA silencing. Nature Rev. Mol. Cell Biol. 9, 22\u201332 (2008)","journal-title":"Nature Rev. Mol. Cell Biol."},{"key":"BFnature07666_CR4","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1038\/nchembio848","volume":"3","author":"NH Tolia","year":"2007","unstructured":"Tolia, N. H. & Joshua-Tor, L. Slicer and the argonautes. Nature Chem. Biol. 3, 36\u201343 (2007)","journal-title":"Nature Chem. Biol."},{"key":"BFnature07666_CR5","doi-asserted-by":"publisher","first-page":"1434","DOI":"10.1126\/science.1102514","volume":"305","author":"JJ Song","year":"2004","unstructured":"Song, J. J., Smith, S. K., Hannon, G. J. & Joshua-Tor, L. Crystal structure of Argonaute and its implications for RISC slicer activity. Science 305, 1434\u20131437 (2004)","journal-title":"Science"},{"key":"BFnature07666_CR6","doi-asserted-by":"publisher","first-page":"1437","DOI":"10.1126\/science.1102513","volume":"305","author":"J Liu","year":"2004","unstructured":"Liu, J. et al. Argonaute2 is the catalytic engine of RNAi. Science 305, 1437\u20131441 (2004)","journal-title":"Science"},{"key":"BFnature07666_CR7","doi-asserted-by":"publisher","first-page":"4727","DOI":"10.1038\/sj.emboj.7600488","volume":"23","author":"JS Parker","year":"2004","unstructured":"Parker, J. S., Roe, S. & Barford, D. Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity. EMBO J. 23, 4727\u20134737 (2004)","journal-title":"EMBO J."},{"key":"BFnature07666_CR8","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1038\/35888","volume":"391","author":"A Fire","year":"1998","unstructured":"Fire, A. et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans . Nature 391, 806\u2013811 (1998)","journal-title":"Nature"},{"key":"BFnature07666_CR9","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1101\/gad.862301","volume":"15","author":"SM Elbashir","year":"2001","unstructured":"Elbashir, S. M., Lendeckel, W. & Tuschl, T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15, 188\u2013200 (2001)","journal-title":"Genes Dev."},{"key":"BFnature07666_CR10","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1101\/gad.1187904","volume":"18","author":"J Martinez","year":"2004","unstructured":"Martinez, J. & Tuschl, T. RISC is a 5\u2032-phosphomonoester-producing RNA endonuclease. Genes Dev. 18, 975\u2013980 (2004)","journal-title":"Genes Dev."},{"key":"BFnature07666_CR11","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1016\/j.cub.2004.03.008","volume":"14","author":"DS Schwarz","year":"2004","unstructured":"Schwarz, D. S., Tomari, Y. & Zamore, P. D. The RNA-induced silencing complex is a Mg2+-dependent endonuclease. Curr. Biol. 14, 787\u2013791 (2004)","journal-title":"Curr. Biol."},{"key":"BFnature07666_CR12","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1101\/gad.1284105","volume":"19","author":"Y Tomari","year":"2005","unstructured":"Tomari, Y. & Zamore, P. D. Perspective: machines for RNAi. Genes Dev. 19, 517\u2013529 (2005)","journal-title":"Genes Dev."},{"key":"BFnature07666_CR13","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1038\/nrd2310","volume":"6","author":"A De Fougerolles","year":"2007","unstructured":"De Fougerolles, A., Vornlocher, H.-P., Maraganore, J. & Lieberman, J. Interfering with disease: a progress report on siRNA-based therapeutics. Nature Rev. Drug. Discovery 6, 443\u2013453 (2007)","journal-title":"Nature Rev. Drug. Discovery"},{"key":"BFnature07666_CR14","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1038\/nrg2006","volume":"8","author":"DH Kim","year":"2007","unstructured":"Kim, D. H. & Rossi, J. J. Strategies for silencing human disease using RNA interference. Nature Rev. Genetics 8, 173\u2013184 (2007)","journal-title":"Nature Rev. Genetics"},{"key":"BFnature07666_CR15","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/j.sbi.2005.05.006","volume":"15","author":"W Filipowicz","year":"2005","unstructured":"Filipowicz, W., Jaskiewicz, L., Kolb, F. A. & Pillai, R. S. Post-transcriptional gene silencing by siRNAs and miRNAs. Curr. Opin. Struct. Biol. 15, 331\u2013341 (2005)","journal-title":"Curr. Opin. Struct. Biol."},{"key":"BFnature07666_CR16","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1016\/j.str.2005.08.005","volume":"13","author":"TM Hall","year":"2005","unstructured":"Hall, T. M. Structure and function of argonaute proteins. Structure 13, 1403\u20131408 (2005)","journal-title":"Structure"},{"key":"BFnature07666_CR17","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1016\/j.tibs.2006.09.010","volume":"31","author":"JS Parker","year":"2006","unstructured":"Parker, J. S. & Barford, D. Argonaute: a scaffold for the function of short regulatory RNAs. Trends Biochem. Sci. 31, 622\u2013630 (2006)","journal-title":"Trends Biochem. Sci."},{"key":"BFnature07666_CR18","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1101\/sqb.2006.71.053","volume":"71","author":"DJ Patel","year":"2006","unstructured":"Patel, D. J. et al. Structural biology of RNA silencing and its functional implications. Cold Spring Harb. Symp. Quant. Biol. 71, 81\u201393 (2006)","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"BFnature07666_CR19","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/j.molcel.2005.07.011","volume":"19","author":"YR Yuan","year":"2005","unstructured":"Yuan, Y. R. et al. Crystal structure of A.  aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage. Mol. Cell 19, 405\u2013419 (2005)","journal-title":"Mol. Cell"},{"key":"BFnature07666_CR20","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1038\/nature07315","volume":"456","author":"Y Wang","year":"2008","unstructured":"Wang, Y. et al. Structure of the guide-strand-containing Argonaute silencing complex. Nature 456, 209\u2013213 (2008)","journal-title":"Nature"},{"key":"BFnature07666_CR21","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1038\/nature03462","volume":"434","author":"JS Parker","year":"2005","unstructured":"Parker, J. S., Roe, S. M. & Barford, D. Structural insights into mRNA recognition from a PIWI domain-siRNA guide complex. Nature 434, 663\u2013666 (2005)","journal-title":"Nature"},{"key":"BFnature07666_CR22","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1038\/nature03514","volume":"434","author":"JB Ma","year":"2005","unstructured":"Ma, J. B. et al. Structural basis for 5\u2032-end-specific recognition of guide RNA by the A.  fulgidus Piwi protein. Nature 434, 666\u2013670 (2005)","journal-title":"Nature"},{"key":"BFnature07666_CR23","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1038\/nature02519","volume":"429","author":"JB Ma","year":"2004","unstructured":"Ma, J. B., Ye, K. & Patel, D. J. Structural basis for overhang-specific small interfering RNA recognition by the Paz domain. Nature 429, 318\u2013322 (2004)","journal-title":"Nature"},{"key":"BFnature07666_CR24","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1038\/nsmb777","volume":"11","author":"A Lingel","year":"2004","unstructured":"Lingel, A., Simon, B., Izaurralde, E. & Sattler, M. Nucleic acid 3\u2032-end recognition by the Argonaute2 Paz domain. Nature Struct. Mol. Biol. 11, 576\u2013577 (2004)","journal-title":"Nature Struct. Mol. Biol."},{"key":"BFnature07666_CR25","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1038\/nsmb780","volume":"11","author":"B Haley","year":"2004","unstructured":"Haley, B. & Zamore, P. D. Kinetic analysis of the RNAi enzyme complex. Nature Struct. Mol. Biol. 11, 599\u2013606 (2004)","journal-title":"Nature Struct. Mol. Biol."},{"key":"BFnature07666_CR26","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1016\/j.cell.2005.04.024","volume":"121","author":"M Nowotny","year":"2005","unstructured":"Nowotny, M., Gaidamakov, S. A., Crouch, R. J. & Yang, W. Crystal structures of RNase H bound to an RNA\/DNA hybrid: substrate specificity and metal-dependent catalysis. Cell 121, 1005\u20131016 (2005)","journal-title":"Cell"},{"key":"BFnature07666_CR27","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/S0092-8674(03)00759-1","volume":"115","author":"DS Schwarz","year":"2003","unstructured":"Schwarz, D. S. et al. Asymmetry in the assembly of the RNAi enzyme complex. Cell 115, 199\u2013208 (2003)","journal-title":"Cell"},{"key":"BFnature07666_CR28","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1261\/rna.789808","volume":"14","author":"PY Chen","year":"2008","unstructured":"Chen, P. Y. et al. Strand-specific 5\u2032-O-methylation of siRNA duplexes controls guide strand selection and targeting specificity. RNA 14, 263\u2013274 (2008)","journal-title":"RNA"},{"key":"BFnature07666_CR29","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1261\/rna.30706","volume":"12","author":"AL Jackson","year":"2006","unstructured":"Jackson, A. L. et al. Position-specific chemical modification of siRNAs reduces \u201coff-target\u201d transcript silencing. RNA 12, 1197\u20131205 (2006)","journal-title":"RNA"},{"key":"BFnature07666_CR30","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1038\/nrd1345","volume":"3","author":"Y Dorsett","year":"2004","unstructured":"Dorsett, Y. & Tuschl, T. siRNAs: applications in functional genomics and potential as therapeutics. Nature Rev. Drug Discov. 3, 318\u2013329 (2004)","journal-title":"Nature Rev. Drug Discov."},{"key":"BFnature07666_CR31","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1038\/nbt831","volume":"21","author":"AL Jackson","year":"2003","unstructured":"Jackson, A. L. et al. Expression profiling reveals off-target gene regulation by RNAi. Nature Biotechnol. 21, 635\u2013637 (2003)","journal-title":"Nature Biotechnol."},{"key":"BFnature07666_CR32","doi-asserted-by":"publisher","first-page":"1587","DOI":"10.1126\/science.1140494","volume":"315","author":"LS Gunawardane","year":"2007","unstructured":"Gunawardane, L. S. et al. A slicer-mediated mechanism for repeat-associated siRNA 5\u2032-end formation in Drosophila . Science 315, 1587\u20131590 (2007)","journal-title":"Science"},{"key":"BFnature07666_CR33","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1016\/S0092-8674(02)00908-X","volume":"110","author":"J Martinez","year":"2002","unstructured":"Martinez, J. et al. Single-stranded anti-sense siRNAs guide target RNA cleavage in RNAi. Cell 110, 563\u2013574 (2002)","journal-title":"Cell"},{"key":"BFnature07666_CR34","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1073\/pnas.0710869105","volume":"105","author":"IJ MacRae","year":"2008","unstructured":"MacRae, I. J. et al. In vitro reconstitution of human RISC-loading complex. Proc. Natl Acad. Sci. USA 105, 512\u2013517 (2008)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature07666_CR35","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.cell.2007.04.037","volume":"130","author":"SL Ameres","year":"2007","unstructured":"Ameres, S. L., Martinez, J. & Schroeder, R. Molecular basis for target RNA recognition and cleavage by human RISC. Cell 130, 101\u2013112 (2007)","journal-title":"Cell"},{"key":"BFnature07666_CR36","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1038\/nrm2085","volume":"8","author":"TM Rana","year":"2007","unstructured":"Rana, T. M. Illuminating the silence: understanding the structure and function of small RNAs. Nature Rev. Mol. Cell Biol. 8, 23\u201326 (2007)","journal-title":"Nature Rev. Mol. Cell Biol."},{"key":"BFnature07666_CR37","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","volume":"276","author":"Z Otwinowski","year":"1997","unstructured":"Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Meth. Enzymol. 276, 307\u2013326 (1997)","journal-title":"Meth. Enzymol."},{"key":"BFnature07666_CR38","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1107\/S0021889807021206","volume":"40","author":"AJ McCoy","year":"2007","unstructured":"McCoy, A. J. et al. Phaser crystallographic software. J. App. Crystallogr. 40, 658\u2013674 (2007)","journal-title":"J. App. Crystallogr."},{"key":"BFnature07666_CR39","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1107\/S0907444998003254","volume":"54","author":"AT Brunger","year":"1998","unstructured":"Brunger, A. T. et al. Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr. D 54, 905\u2013921 (1998)","journal-title":"Acta Crystallogr. D"},{"key":"BFnature07666_CR40","doi-asserted-by":"publisher","first-page":"2126","DOI":"10.1107\/S0907444904019158","volume":"60","author":"P Emsley","year":"2004","unstructured":"Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Acta Crystallogr. D 60, 2126\u20132132 (2004)","journal-title":"Acta Crystallogr. D"},{"key":"BFnature07666_CR41","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1107\/S0108767390010224","volume":"47","author":"TA Jones","year":"1991","unstructured":"Jones, T. A., Zhou, J. Y., Cowan, S. W. & Kjeldgaard, M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. A 47, 110\u2013119 (1991)","journal-title":"Acta Crystallogr. A"}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nature07666.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature07666","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature07666.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T14:21:39Z","timestamp":1684419699000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature07666"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":41,"journal-issue":{"issue":"7224","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["BFnature07666"],"URL":"https:\/\/doi.org\/10.1038\/nature07666","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1158554.618744","asserted-by":"object"}]},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12]]}}}