{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T08:27:45Z","timestamp":1777710465701,"version":"3.51.4"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"7339","license":[{"start":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T00:00:00Z","timestamp":1292976000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2011,3]]},"DOI":"10.1038\/nature09725","type":"journal-article","created":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T11:29:07Z","timestamp":1293017347000},"page":"480-485","source":"Crossref","is-referenced-by-count":790,"title":["Comprehensive analysis of the chromatin landscape in Drosophila melanogaster"],"prefix":"10.1038","volume":"471","author":[{"given":"Peter V.","family":"Kharchenko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artyom A.","family":"Alekseyenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuri B.","family":"Schwartz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aki","family":"Minoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole C.","family":"Riddle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Ernst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Sabo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica","family":"Larschan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey A.","family":"Gorchakov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Linder-Basso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Plachetka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"Shanower","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael Y.","family":"Tolstorukov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lovelace J.","family":"Luquette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruibin","family":"Xi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youngsook L.","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard W.","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric P.","family":"Bishop","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresa K.","family":"Canfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Sandstrom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Thurman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"MacAlpine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"Stamatoyannopoulos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manolis","family":"Kellis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah C. R.","family":"Elgin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitzi I.","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Pirrotta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary H.","family":"Karpen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,12,22]]},"reference":[{"key":"BFnature09725_CR1","unstructured":"modENCODE Identification of functional elements and regulatory circuits by Drosophila modENCODE. Science 10.1126\/science.1198374. (in the press)"},{"key":"BFnature09725_CR2","unstructured":"Gerstein, M. B. et al. Integrative analysis of the Caenorhabditis elegans genome by the modENCODE project. Science 10.1126\/science.1196914. (in the press)"},{"key":"BFnature09725_CR3","doi-asserted-by":"publisher","first-page":"2185","DOI":"10.1126\/science.287.5461.2185","volume":"287","author":"MD Adams","year":"2000","unstructured":"Adams, M. D. et al. The genome sequence of Drosophila melanogaster . Science 287, 2185\u20132195 (2000)","journal-title":"Science"},{"key":"BFnature09725_CR4","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1038\/nature06341","volume":"450","author":"AG Clark","year":"2007","unstructured":"Clark, A. G. et al. Evolution of genes and genomes on the Drosophila phylogeny. Nature 450, 203\u2013218 (2007)","journal-title":"Nature"},{"key":"BFnature09725_CR5","doi-asserted-by":"publisher","first-page":"1625","DOI":"10.1126\/science.1139816","volume":"316","author":"RA Hoskins","year":"2007","unstructured":"Hoskins, R. A. et al. Sequence finishing and mapping of Drosophila melanogaster heterochromatin. Science 316, 1625\u20131628 (2007)","journal-title":"Science"},{"key":"BFnature09725_CR6","doi-asserted-by":"publisher","first-page":"D555","DOI":"10.1093\/nar\/gkn788","volume":"37","author":"S Tweedie","year":"2009","unstructured":"Tweedie, S. et al. FlyBase: enhancing Drosophila Gene Ontology annotations. Nucleic Acids Res. 37, D555\u2013D559 (2009)","journal-title":"Nucleic Acids Res."},{"key":"BFnature09725_CR7","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1038\/nature01411","volume":"421","author":"G Felsenfeld","year":"2003","unstructured":"Felsenfeld, G. & Groudine, M. Controlling the double helix. Nature 421, 448\u2013453 (2003)","journal-title":"Nature"},{"key":"BFnature09725_CR8","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.gde.2008.01.010","volume":"18","author":"EM Mendenhall","year":"2008","unstructured":"Mendenhall, E. M. & Bernstein, B. E. Chromatin state maps: new technologies, new insights. Curr. Opin. Genet. Dev. 18, 109\u2013115 (2008)","journal-title":"Curr. Opin. Genet. Dev."},{"key":"BFnature09725_CR9","unstructured":"Egelhofer, T. A. et al. An assessment of histone-modification antibody quality. Nature Struct. Mol. Biol. 10.1038\/nsmb.1972 (5 December 2010)"},{"key":"BFnature09725_CR10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S1937-6448(08)01801-7","volume":"273","author":"JC Eissenberg","year":"2009","unstructured":"Eissenberg, J. C. & Reuter, G. Cellular mechanism for targeting heterochromatin formation in Drosophila . Int. Rev. Cell Mol. Biol. 273, 1\u201347 (2009)","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"BFnature09725_CR11","doi-asserted-by":"publisher","first-page":"266","DOI":"10.1016\/j.ceb.2008.03.002","volume":"20","author":"YB Schwartz","year":"2008","unstructured":"Schwartz, Y. B. & Pirrotta, V. Polycomb complexes and epigenetic states. Curr. Opin. Cell Biol. 20, 266\u2013273 (2008)","journal-title":"Curr. Opin. Cell Biol."},{"key":"BFnature09725_CR12","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1016\/j.cell.2007.01.015","volume":"128","author":"B Li","year":"2007","unstructured":"Li, B., Carey, M. & Workman, J. L. The role of chromatin during transcription. Cell 128, 707\u2013719 (2007)","journal-title":"Cell"},{"key":"BFnature09725_CR13","doi-asserted-by":"publisher","first-page":"e328","DOI":"10.1371\/journal.pbio.0030328","volume":"3","author":"CL Liu","year":"2005","unstructured":"Liu, C. L. et al. Single-nucleosome mapping of histone modifications in S. cerevisiae . PLoS Biol. 3, e328 (2005)","journal-title":"PLoS Biol."},{"key":"BFnature09725_CR14","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1016\/j.cell.2007.05.009","volume":"129","author":"A Barski","year":"2007","unstructured":"Barski, A. et al. High-resolution profiling of histone methylations in the human genome. Cell 129, 823\u2013837 (2007)","journal-title":"Cell"},{"key":"BFnature09725_CR15","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.molcel.2007.08.011","volume":"28","author":"E Larschan","year":"2007","unstructured":"Larschan, E. et al. MSL complex is attracted to genes marked by H3K36 trimethylation using a sequence-independent mechanism. Mol. Cell 28, 121\u2013133 (2007)","journal-title":"Mol. Cell"},{"key":"BFnature09725_CR16","unstructured":"Riddle, N. C. et al. Plasticity in patterns of histone modifications and chromosomal proteins in Drosophila heterochromatin. Genome Res. 10.1101\/gr.110098.110. (in the press)"},{"key":"BFnature09725_CR17","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1093\/bioinformatics\/btp152","volume":"25","author":"S Anders","year":"2009","unstructured":"Anders, S. Visualization of genomic data with the Hilbert curve. Bioinformatics 25, 1231\u20131235 (2009)","journal-title":"Bioinformatics"},{"key":"BFnature09725_CR18","doi-asserted-by":"publisher","first-page":"3094","DOI":"10.1101\/gad.1246404","volume":"18","author":"DM MacAlpine","year":"2004","unstructured":"MacAlpine, D. M., Rodriguez, H. K. & Bell, S. P. Coordination of replication and transcription along a Drosophila chromosome. Genes Dev. 18, 3094\u20133105 (2004)","journal-title":"Genes Dev."},{"key":"BFnature09725_CR19","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1101\/SQB.1974.038.01.024","volume":"38","author":"AB Blumenthal","year":"1974","unstructured":"Blumenthal, A. B., Kriegstein, H. J. & Hogness, D. S. The units of DNA replication in Drosophila melanogaster chromosomes. Cold Spring Harb. Symp. Quant. Biol. 38, 205\u2013223 (1974)","journal-title":"Cold Spring Harb. Symp. Quant. Biol."},{"key":"BFnature09725_CR20","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1038\/nbt.1662","volume":"28","author":"J Ernst","year":"2010","unstructured":"Ernst, J. & Kellis, M. Discovery and characterization of chromatin states for systematic annotation of the human genome. Nature Biotechnol. 28, 817\u2013825 (2010)","journal-title":"Nature Biotechnol."},{"key":"BFnature09725_CR21","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/s00412-007-0129-1","volume":"117","author":"Z Misulovin","year":"2008","unstructured":"Misulovin, Z. et al. Association of cohesin and Nipped-B with transcriptionally active regions of the Drosophila melanogaster genome. Chromosoma 117, 89\u2013102 (2008)","journal-title":"Chromosoma"},{"key":"BFnature09725_CR22","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1038\/nature09380","volume":"467","author":"MH Kagey","year":"2010","unstructured":"Kagey, M. H. et al. Mediator and cohesin connect gene expression and chromatin architecture. Nature 467, 430\u2013435 (2010)","journal-title":"Nature"},{"key":"BFnature09725_CR23","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1126\/science.1186777","volume":"328","author":"RB Deal","year":"2010","unstructured":"Deal, R. B., Henikoff, J. G. & Henikoff, S. Genome-wide kinetics of nucleosome turnover determined by metabolic labeling of histones. Science 328, 1161\u20131164 (2010)","journal-title":"Science"},{"key":"BFnature09725_CR24","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1101\/gr.087619.108","volume":"19","author":"S Henikoff","year":"2009","unstructured":"Henikoff, S., Henikoff, J. G., Sakai, A., Loeb, G. B. & Ahmad, K. Genome-wide profiling of salt fractions maps physical properties of chromatin. Genome Res. 19, 460\u2013469 (2009)","journal-title":"Genome Res."},{"key":"BFnature09725_CR25","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1101\/gr.097873.109","volume":"20","author":"HK MacAlpine","year":"2010","unstructured":"MacAlpine, H. K., Gordan, R., Powell, S. K., Hartemink, A. J. & MacAlpine, D. M. Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading. Genome Res. 20, 201\u2013211 (2010)","journal-title":"Genome Res."},{"key":"BFnature09725_CR26","doi-asserted-by":"publisher","first-page":"854","DOI":"10.1038\/nature07730","volume":"457","author":"A Visel","year":"2009","unstructured":"Visel, A. et al. ChIP-seq accurately predicts tissue-specific activity of enhancers. Nature 457, 854\u2013858 (2009)","journal-title":"Nature"},{"key":"BFnature09725_CR27","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1038\/nature08531","volume":"462","author":"RP Zinzen","year":"2009","unstructured":"Zinzen, R. P., Girardot, C., Gagneur, J., Braun, M. & Furlong, E. E. Combinatorial binding predicts spatio-temporal cis-regulatory activity. Nature 462, 65\u201370 (2009)","journal-title":"Nature"},{"key":"BFnature09725_CR28","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1038\/ng1817","volume":"38","author":"YB Schwartz","year":"2006","unstructured":"Schwartz, Y. B. et al. Genome-wide analysis of Polycomb targets in Drosophila melanogaster . Nature Genet. 38, 700\u2013705 (2006)","journal-title":"Nature Genet."},{"key":"BFnature09725_CR29","doi-asserted-by":"publisher","first-page":"e1000805","DOI":"10.1371\/journal.pgen.1000805","volume":"6","author":"YB Schwartz","year":"2010","unstructured":"Schwartz, Y. B. et al. Alternative epigenetic chromatin states of Polycomb target genes. PLoS Genet. 6, e1000805 (2010)","journal-title":"PLoS Genet."},{"key":"BFnature09725_CR30","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1126\/science.1181421","volume":"327","author":"S Nechaev","year":"2010","unstructured":"Nechaev, S. et al. Global analysis of short RNAs reveals widespread promoter-proximal stalling and arrest of Pol II in Drosophila . Science 327, 335\u2013338 (2010)","journal-title":"Science"},{"key":"BFnature09725_CR31","doi-asserted-by":"publisher","first-page":"1845","DOI":"10.1126\/science.1162228","volume":"322","author":"LJ Core","year":"2008","unstructured":"Core, L. J., Waterfall, J. J. & Lis, J. T. Nascent RNA sequencing reveals widespread pausing and divergent initiation at human promoters. Science 322, 1845\u20131848 (2008)","journal-title":"Science"},{"key":"BFnature09725_CR32","doi-asserted-by":"publisher","first-page":"854","DOI":"10.1038\/286854a0","volume":"286","author":"C Wu","year":"1980","unstructured":"Wu, C. The 5\u2032 ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I. Nature 286, 854\u2013860 (1980)","journal-title":"Nature"},{"key":"BFnature09725_CR33","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1016\/0092-8674(79)90095-3","volume":"16","author":"C Wu","year":"1979","unstructured":"Wu, C., Bingham, P. M., Livak, K. J., Holmgren, R. & Elgin, S. C. The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence. Cell 16, 797\u2013806 (1979)","journal-title":"Cell"},{"key":"BFnature09725_CR34","doi-asserted-by":"crossref","first-page":"19259","DOI":"10.1016\/S0021-9258(19)77625-X","volume":"263","author":"SC Elgin","year":"1988","unstructured":"Elgin, S. C. The formation and function of DNase I hypersensitive sites in the process of gene activation. J. Biol. Chem. 263, 19259\u201319262 (1988)","journal-title":"J. Biol. Chem."},{"key":"BFnature09725_CR35","doi-asserted-by":"publisher","first-page":"941","DOI":"10.1038\/ng.409","volume":"41","author":"C Jin","year":"2009","unstructured":"Jin, C. et al. H3.3\/H2A.Z double variant-containing nucleosomes mark \u2018nucleosome-free regions\u2019 of active promoters and other regulatory regions. Nature Genet. 41, 941\u2013945 (2009)","journal-title":"Nature Genet."},{"key":"BFnature09725_CR36","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1038\/nmeth.1313","volume":"6","author":"JR Hesselberth","year":"2009","unstructured":"Hesselberth, J. R. et al. Global mapping of protein-DNA interactions in vivo by digital genomic footprinting. Nature Methods 6, 283\u2013289 (2009)","journal-title":"Nature Methods"},{"key":"BFnature09725_CR37","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1038\/nature07829","volume":"459","author":"ND Heintzman","year":"2009","unstructured":"Heintzman, N. D. et al. Histone modifications at human enhancers reflect global cell-type-specific gene expression. Nature 459, 108\u2013112 (2009)","journal-title":"Nature"},{"key":"BFnature09725_CR38","doi-asserted-by":"publisher","first-page":"R80","DOI":"10.1186\/gb-2009-10-7-r80","volume":"10","author":"S MacArthur","year":"2009","unstructured":"MacArthur, S. et al. Developmental roles of 21 Drosophila transcription factors are determined by quantitative differences in binding to an overlapping set of thousands of genomic regions. Genome Biol. 10, R80 (2009)","journal-title":"Genome Biol."},{"key":"BFnature09725_CR39","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1038\/nature09033","volume":"465","author":"TK Kim","year":"2010","unstructured":"Kim, T. K. et al. Widespread transcription at neuronal activity-regulated enhancers. Nature 465, 182\u2013187 (2010)","journal-title":"Nature"},{"key":"BFnature09725_CR40","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/j.cell.2010.09.009","volume":"143","author":"GJ Filion","year":"2010","unstructured":"Filion, G. J. et al. Systematic protein location mapping reveals five principal chromatin types in Drosophila cells. Cell 143, 212\u2013224 (2010)","journal-title":"Cell"},{"key":"BFnature09725_CR41","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/j.cell.2006.02.041","volume":"125","author":"BE Bernstein","year":"2006","unstructured":"Bernstein, B. E. et al. A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell 125, 315\u2013326 (2006)","journal-title":"Cell"},{"key":"BFnature09725_CR42","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.molcel.2010.03.019","volume":"38","author":"A Kanhere","year":"2010","unstructured":"Kanhere, A. et al. Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2. Mol. Cell 38, 675\u2013688 (2010)","journal-title":"Mol. Cell"},{"key":"BFnature09725_CR43","doi-asserted-by":"publisher","first-page":"e13","DOI":"10.1371\/journal.pbio.1000013","volume":"7","author":"B Schuettengruber","year":"2009","unstructured":"Schuettengruber, B. et al. Functional anatomy of polycomb and trithorax chromatin landscapes in Drosophila embryos. PLoS Biol. 7, e13 (2009)","journal-title":"PLoS Biol."},{"key":"BFnature09725_CR44","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1016\/j.immuni.2009.08.021","volume":"31","author":"M Sekimata","year":"2009","unstructured":"Sekimata, M. et al. CCCTC-binding factor and the transcription factor T-bet orchestrate T helper 1 cell-specific structure and function at the interferon-\u03b3 locus. Immunity 31, 551\u2013564 (2009)","journal-title":"Immunity"},{"key":"BFnature09725_CR45","unstructured":"Cherbas, L. et al. The transcriptional diversity of 25 Drosophila cell lines. Genome Res. 21 10.1101\/gr.112961.110 (in the press)"},{"key":"BFnature09725_CR46","unstructured":"Gravely, B. R. et al. The developmental transcriptome of Drosophila melanogaster . Nature 10.1038\/nature09715 (this issue)."},{"key":"BFnature09725_CR47","doi-asserted-by":"publisher","first-page":"6499","DOI":"10.1073\/pnas.110149597","volume":"97","author":"JC Clemens","year":"2000","unstructured":"Clemens, J. C. et al. Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl Acad. Sci. USA 97, 6499\u20136503 (2000)","journal-title":"Proc. Natl Acad. Sci. USA"}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nature09725.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature09725","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature09725.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T17:49:43Z","timestamp":1684432183000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature09725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,22]]},"references-count":47,"journal-issue":{"issue":"7339","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["BFnature09725"],"URL":"https:\/\/doi.org\/10.1038\/nature09725","relation":{},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,22]]}}}