{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T14:02:35Z","timestamp":1777644155462,"version":"3.51.4"},"reference-count":46,"publisher":"Springer Science and Business Media LLC","issue":"7632","license":[{"start":{"date-parts":[[2016,11,30]],"date-time":"2016-11-30T00:00:00Z","timestamp":1480464000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nature"],"published-print":{"date-parts":[[2016,12]]},"DOI":"10.1038\/nature20607","type":"journal-article","created":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T15:12:34Z","timestamp":1480432354000},"page":"292-295","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":157,"title":["Designed proteins induce the formation of nanocage-containing extracellular vesicles"],"prefix":"10.1038","volume":"540","author":[{"given":"J\u00f6rg","family":"Votteler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassandra","family":"Ogohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sue","family":"Yi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Hsia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Una","family":"Nattermann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Belnap","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil P.","family":"King","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wesley I.","family":"Sundquist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,11,30]]},"reference":[{"key":"BFnature20607_CR1","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1038\/nature04586","volume":"440","author":"PW Rothemund","year":"2006","unstructured":"Rothemund, P. W. Folding DNA to create nanoscale shapes and patterns. Nature 440, 297\u2013302 (2006)","journal-title":"Nature"},{"key":"BFnature20607_CR2","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1126\/science.1227268","volume":"338","author":"Y Ke","year":"2012","unstructured":"Ke, Y., Ong, L. L., Shih, W. M. & Yin, P. Three-dimensional structures self-assembled from DNA bricks. Science 338, 1177\u20131183 (2012)","journal-title":"Science"},{"key":"BFnature20607_CR3","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1126\/science.1219364","volume":"336","author":"NP King","year":"2012","unstructured":"King, N. P. et al. Computational design of self-assembling protein nanomaterials with atomic level accuracy. Science 336, 1171\u20131174 (2012)","journal-title":"Science"},{"key":"BFnature20607_CR4","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1038\/nature17633","volume":"533","author":"Y Suzuki","year":"2016","unstructured":"Suzuki, Y. et al. Self-assembly of coherently dynamic, auxetic, two-dimensional protein crystals. Nature 533, 369\u2013373 (2016)","journal-title":"Nature"},{"key":"BFnature20607_CR5","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.1038\/nchem.2107","volume":"6","author":"YT Lai","year":"2014","unstructured":"Lai, Y. T. et al. Structure of a designed protein cage that self-assembles into a highly porous cube. Nat. Chem. 6, 1065\u20131071 (2014)","journal-title":"Nat. Chem."},{"key":"BFnature20607_CR6","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1038\/nature14874","volume":"525","author":"Y Mou","year":"2015","unstructured":"Mou, Y., Yu, J. Y., Wannier, T. M., Guo, C. L. & Mayo, S. L. Computational design of co-assembling protein-DNA nanowires. Nature 525, 230\u2013233 (2015)","journal-title":"Nature"},{"key":"BFnature20607_CR7","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1126\/science.1206938","volume":"333","author":"CJ Delebecque","year":"2011","unstructured":"Delebecque, C. J., Lindner, A. B., Silver, P. A. & Aldaye, F. A. Organization of intracellular reactions with rationally designed RNA assemblies. Science 333, 470\u2013474 (2011)","journal-title":"Science"},{"key":"BFnature20607_CR8","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/j.chom.2013.08.012","volume":"14","author":"J Votteler","year":"2013","unstructured":"Votteler, J. & Sundquist, W. I. Virus budding and the ESCRT pathway. Cell Host Microbe 14, 232\u2013241 (2013)","journal-title":"Cell Host Microbe"},{"key":"BFnature20607_CR9","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1038\/nature18010","volume":"535","author":"Y Hsia","year":"2016","unstructured":"Hsia, Y. et al. Design of a hyperstable 60-subunit protein icosahedron. Nature 535, 136\u2013139 (2016)","journal-title":"Nature"},{"key":"BFnature20607_CR10","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1097\/00002030-199106000-00002","volume":"5","author":"JW Wills","year":"1991","unstructured":"Wills, J. W. & Craven, R. C. Form, function, and use of retroviral gag proteins. AIDS 5, 639\u2013654 (1991)","journal-title":"AIDS"},{"key":"BFnature20607_CR11","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.coviro.2013.03.011","volume":"3","author":"W Weissenhorn","year":"2013","unstructured":"Weissenhorn, W., Poudevigne, E., Effantin, G. & Bassereau, P. How to get out: ssRNA enveloped viruses and membrane fission. Curr. Opin. Virol . 3, 159\u2013167 (2013)","journal-title":"Curr. Opin. Virol"},{"key":"BFnature20607_CR12","doi-asserted-by":"publisher","first-page":"5781","DOI":"10.1073\/pnas.86.15.5781","volume":"86","author":"HG G\u00f6ttlinger","year":"1989","unstructured":"G\u00f6ttlinger, H. G., Sodroski, J. G. & Haseltine, W. A. Role of capsid precursor processing and myristoylation in morphogenesis and infectivity of human immunodeficiency virus type 1. Proc. Natl Acad. Sci. USA 86, 5781\u20135785 (1989)","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFnature20607_CR13","doi-asserted-by":"publisher","first-page":"1554","DOI":"10.1002\/pmic.201200329","volume":"13","author":"DS Choi","year":"2013","unstructured":"Choi, D. S., Kim, D. K., Kim, Y. K. & Gho, Y. S. Proteomics, transcriptomics and lipidomics of exosomes and ectosomes. Proteomics 13, 1554\u20131571 (2013)","journal-title":"Proteomics"},{"key":"BFnature20607_CR14","doi-asserted-by":"publisher","first-page":"9039","DOI":"10.1128\/JVI.01013-06","volume":"80","author":"E Chertova","year":"2006","unstructured":"Chertova, E. et al. Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages. J. Virol. 80, 9039\u20139052 (2006)","journal-title":"J. Virol."},{"key":"BFnature20607_CR15","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1038\/nbt745","volume":"20","author":"M Cavrois","year":"2002","unstructured":"Cavrois, M., De Noronha, C. & Greene, W. C. A sensitive and specific enzyme-based assay detecting HIV-1 virion fusion in primary T lymphocytes. Nat. Biotechnol. 20, 1151\u20131154 (2002)","journal-title":"Nat. Biotechnol."},{"key":"BFnature20607_CR16","doi-asserted-by":"publisher","first-page":"10645","DOI":"10.1128\/JVI.77.19.10645-10650.2003","volume":"77","author":"M Tobiume","year":"2003","unstructured":"Tobiume, M., Lineberger, J. E., Lundquist, C. A., Miller, M. D. & Aiken, C. Nef does not affect the efficiency of human immunodeficiency virus type 1 fusion with target cells. J. Virol. 77, 10645\u201310650 (2003)","journal-title":"J. Virol."},{"key":"BFnature20607_CR17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1128\/JVI.70.1.159-164.1996","volume":"70","author":"E Kondo","year":"1996","unstructured":"Kondo, E. & G\u00f6ttlinger, H. G. A conserved LXXLF sequence is the major determinant in p6gag required for the incorporation of human immunodeficiency virus type 1 Vpr. J. Virol. 70, 159\u2013164 (1996)","journal-title":"J. Virol."},{"key":"BFnature20607_CR18","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1128\/JVI.69.3.1435-1443.1995","volume":"69","author":"BL Fredericksen","year":"1995","unstructured":"Fredericksen, B. L. & Whitt, M. A. Vesicular stomatitis virus glycoprotein mutations that affect membrane fusion activity and abolish virus infectivity. J. Virol. 69, 1435\u20131443 (1995)","journal-title":"J. Virol."},{"key":"BFnature20607_CR19","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1016\/S0092-8674(03)00653-6","volume":"114","author":"B Strack","year":"2003","unstructured":"Strack, B., Calistri, A., Craig, S., Popova, E. & G\u00f6ttlinger, H. G. AIP1\/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell 114, 689\u2013699 (2003)","journal-title":"Cell"},{"key":"BFnature20607_CR20","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1038\/nm1201-1313","volume":"7","author":"J Martin-Serrano","year":"2001","unstructured":"Martin-Serrano, J., Zang, T. & Bieniasz, P. D. HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress. Nat. Med. 7, 1313\u20131319 (2001)","journal-title":"Nat. Med."},{"key":"BFnature20607_CR21","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1016\/j.jmb.2015.11.004","volume":"428","author":"N Monroe","year":"2016","unstructured":"Monroe, N. & Hill, C. P. Meiotic clade AAA ATPases: protein polymer disassembly machines. J. Mol. Biol. 428 (9 Pt B), 1897\u20131911 (2016)","journal-title":"J. Mol. Biol."},{"key":"BFnature20607_CR22","doi-asserted-by":"crossref","first-page":"5455","DOI":"10.1128\/JVI.69.9.5455-5460.1995","volume":"69","author":"LJ Parent","year":"1995","unstructured":"Parent, L. J. et al. Positionally independent and exchangeable late budding functions of the Rous sarcoma virus and human immunodeficiency virus Gag proteins. J. Virol. 69, 5455\u20135460 (1995)","journal-title":"J. Virol."},{"key":"BFnature20607_CR23","doi-asserted-by":"publisher","first-page":"5854","DOI":"10.1038\/ncomms6854","volume":"6","author":"GA Frank","year":"2015","unstructured":"Frank, G. A. et al. Maturation of the HIV-1 core by a non-diffusional phase transition. Nat. Commun. 6, 5854 (2015)","journal-title":"Nat. Commun."},{"key":"BFnature20607_CR24","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1038\/nature13806","volume":"517","author":"X Wang","year":"2015","unstructured":"Wang, X. et al. Hepatitis A virus and the origins of picornaviruses. Nature 517, 85\u201388 (2015)","journal-title":"Nature"},{"key":"BFnature20607_CR25","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1038\/nature12029","volume":"496","author":"Z Feng","year":"2013","unstructured":"Feng, Z. et al. A pathogenic picornavirus acquires an envelope by hijacking cellular membranes. Nature 496, 367\u2013371 (2013)","journal-title":"Nature"},{"key":"BFnature20607_CR26","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1038\/nature05840","volume":"447","author":"BJ Reynwar","year":"2007","unstructured":"Reynwar, B. J. et al. Aggregation and vesiculation of membrane proteins by curvature-mediated interactions. Nature 447, 461\u2013464 (2007)","journal-title":"Nature"},{"key":"BFnature20607_CR27","doi-asserted-by":"publisher","first-page":"5132","DOI":"10.1021\/nn5011914","volume":"8","author":"SD Perrault","year":"2014","unstructured":"Perrault, S. D. & Shih, W. M. Virus-inspired membrane encapsulation of DNA nanostructures to achieve in vivo stability. ACS Nano 8, 5132\u20135140 (2014)","journal-title":"ACS Nano"},{"key":"BFnature20607_CR28","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1038\/nature15373","volume":"526","author":"CM Hu","year":"2015","unstructured":"Hu, C. M. et al. Nanoparticle biointerfacing by platelet membrane cloaking. Nature 526, 118\u2013121 (2015)","journal-title":"Nature"},{"key":"BFnature20607_CR29","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1038\/nrd3978","volume":"12","author":"S EL Andaloussi","year":"2013","unstructured":"EL Andaloussi, S., M\u00e4ger, I., Breakefield, X. O. & Wood, M. J. A. Extracellular vesicles: biology and emerging therapeutic opportunities. Nat. Rev. Drug Discov. 12, 347\u2013357 (2013)","journal-title":"Nat. Rev. Drug Discov."},{"key":"BFnature20607_CR30","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1146\/annurev-pharmtox-010814-124630","volume":"55","author":"B Gy\u00f6rgy","year":"2015","unstructured":"Gy\u00f6rgy, B., Hung, M. E., Breakefield, X. O. & Leonard, J. N. Therapeutic applications of extracellular vesicles: clinical promise and open questions. Annu. Rev. Pharmacol. Toxicol. 55, 439\u2013464 (2015)","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"BFnature20607_CR31","first-page":"1","volume":"0","author":"E Morita","year":"2012","unstructured":"Morita, E., Arii, J., Christensen, D., Votteler, J. & Sundquist, W. I. Attenuated protein expression vectors for use in siRNA rescue experiments. Biotechniques 0, 1\u20135 (2012)","journal-title":"Biotechniques"},{"key":"BFnature20607_CR32","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1038\/nmeth.1318","volume":"6","author":"DG Gibson","year":"2009","unstructured":"Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343\u2013345 (2009)","journal-title":"Nat. Methods"},{"key":"BFnature20607_CR33","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671\u2013675 (2012)","journal-title":"Nat. Methods"},{"key":"BFnature20607_CR34","doi-asserted-by":"crossref","unstructured":"Thery, C., Amigorena, S., Raposo, G. & Clayton, A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr. Protoc. Cell Biol. Chapter 3, Unit 3 22 (2006)","DOI":"10.1002\/0471143030.cb0322s30"},{"key":"BFnature20607_CR35","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/S0968-0896(01)00307-8","volume":"10","author":"JS Griffiths","year":"2002","unstructured":"Griffiths, J. S. et al. Cloning, isolation and characterization of the Thermotoga maritima KDPG aldolase. Bioorg. Med. Chem. 10, 545\u2013550 (2002)","journal-title":"Bioorg. Med. Chem."},{"key":"BFnature20607_CR36","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.jsb.2005.07.007","volume":"152","author":"DN Mastronarde","year":"2005","unstructured":"Mastronarde, D. N. Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36\u201351 (2005)","journal-title":"J. Struct. Biol."},{"key":"BFnature20607_CR37","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","volume":"116","author":"JR Kremer","year":"1996","unstructured":"Kremer, J. R., Mastronarde, D. N. & McIntosh, J. R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71\u201376 (1996)","journal-title":"J. Struct. Biol."},{"key":"BFnature20607_CR38","doi-asserted-by":"publisher","unstructured":"Zheng, S. Q., Palovcak, E., Armache, J., Cheng, Y. & Agard, D. A. Anisotropic correction of beam-induced motion for improved single-particle electron cryo-microscopy. Preprint at http:\/\/dx.doi.org\/10.1101\/061960 (2016)","DOI":"10.1101\/061960"},{"key":"BFnature20607_CR39","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.jsb.2016.04.010","volume":"195","author":"de la Rosa-Trev\u00edn","year":"2016","unstructured":"de la Rosa-Trev\u00edn et al. Scipion: a software framework toward integration, reproducibility and validation in 3D electron microscopy. J. Struct. Biol . 195, 93\u201399 (2016)","journal-title":"J. Struct. Biol"},{"key":"BFnature20607_CR40","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","volume":"192","author":"A Rohou","year":"2015","unstructured":"Rohou, A. & Grigorieff, N. CTFFIND4: fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192, 216\u2013221 (2015)","journal-title":"J. Struct. Biol."},{"key":"BFnature20607_CR41","doi-asserted-by":"publisher","first-page":"2460","DOI":"10.1093\/bioinformatics\/btt429","volume":"29","author":"V Abrishami","year":"2013","unstructured":"Abrishami, V. et al. A pattern matching approach to the automatic selection of particles from low-contrast electron micrographs. Bioinformatics 29, 2460\u20132468 (2013)","journal-title":"Bioinformatics"},{"key":"BFnature20607_CR42","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/j.jsb.2013.09.015","volume":"184","author":"JM de la Rosa-Trev\u00edn","year":"2013","unstructured":"de la Rosa-Trev\u00edn, J. M. et al. Xmipp 3.0: an improved software suite for image processing in electron microscopy. J. Struct. Biol. 184, 321\u2013328 (2013)","journal-title":"J. Struct. Biol."},{"key":"BFnature20607_CR43","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/j.jsb.2012.09.006","volume":"180","author":"SH Scheres","year":"2012","unstructured":"Scheres, S. H. RELION: implementation of a Bayesian approach to cryo-EM structure determination. J. Struct. Biol. 180, 519\u2013530 (2012)","journal-title":"J. Struct. Biol."},{"key":"BFnature20607_CR44","doi-asserted-by":"publisher","first-page":"2891","DOI":"10.1093\/bioinformatics\/btu404","volume":"30","author":"J Vargas","year":"2014","unstructured":"Vargas, J., \u00c1lvarez-Cabrera, A. L., Marabini, R., Carazo, J. M. & Sorzano, C. O. Efficient initial volume determination from electron microscopy images of single particles. Bioinformatics 30, 2891\u20132898 (2014)","journal-title":"Bioinformatics"},{"key":"BFnature20607_CR45","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jcc.20084","volume":"25","author":"EF Pettersen","year":"2004","unstructured":"Pettersen, E. F. et al. UCSF Chimera--a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605\u20131612 (2004)","journal-title":"J. Comput. Chem."},{"key":"BFnature20607_CR46","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/S0076-6879(10)82003-8","volume":"482","author":"PA Penczek","year":"2010","unstructured":"Penczek, P. A. Resolution measures in molecular electron microscopy. Methods Enzymol . 482, 73\u2013100 (2010)","journal-title":"Methods Enzymol"}],"container-title":["Nature"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nature20607.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature20607","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nature20607.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T17:58:09Z","timestamp":1684432689000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nature20607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,30]]},"references-count":46,"journal-issue":{"issue":"7632","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["BFnature20607"],"URL":"https:\/\/doi.org\/10.1038\/nature20607","relation":{},"ISSN":["0028-0836","1476-4687"],"issn-type":[{"value":"0028-0836","type":"print"},{"value":"1476-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,30]]},"assertion":[{"value":"8 July 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 October 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 November 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 December 2016","order":4,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Correction","order":5,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Minor corrections were made to Fig. 4c and the legend of Fig. 3a.","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"J.V., Y.H., N.P.K. and W.I.S. co-authored a US patent application (62\/126,331) with claims relating to Enveloped Protein Nanocages through the CoMotion office at the University of Washington. The authors declare no other competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}