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Med."],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:p>Approximately 50 million people suffer from sepsis yearly, and 13 million die from it. For every hour a patient with septic shock is untreated, their survival rate decreases by 8%. Therefore, rapid detection and antibiotic susceptibility profiling of bacterial agents in the blood of sepsis patients are crucial for determining appropriate treatment. Here, we introduce a method to isolate bacteria from whole blood with high separation efficiency through <jats:italic>Smart centrifugation<\/jats:italic>, followed by microfluidic trapping and subsequent detection using deep learning applied to microscopy images. We detected, within 2 h, <jats:italic>E. coli<\/jats:italic>, <jats:italic>K. pneumoniae<\/jats:italic>, or <jats:italic>E. faecalis<\/jats:italic> from spiked samples of healthy human donor blood at clinically relevant concentrations as low as 9, 7 and 32 colony-forming units per ml of blood, respectively. However, the detection of <jats:italic>S. aureus<\/jats:italic> remains a challenge. This rapid isolation and detection represents a significant advancement towards culture-free detection of bloodstream infections.<\/jats:p>","DOI":"10.1038\/s41746-025-01948-w","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T02:54:59Z","timestamp":1756090499000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Culture-free detection of bacteria from blood for rapid sepsis diagnosis"],"prefix":"10.1038","volume":"8","author":[{"given":"M. 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