{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T19:36:50Z","timestamp":1767382610885},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,2,3]],"date-time":"2009-02-03T00:00:00Z","timestamp":1233619200000},"content-version":"vor","delay-in-days":3808,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The effects of two chemically unrelated nitric oxide (NO)\u2010releasing compounds were studied on prostacyclin production in lipopolysaccharide (LPS)\u2010stimulated human umbilical vein endothelial cells (HUVECs). The cells expressed cyclooxygenase\u20102 (COX\u20102) protein and produced prostacyclin by NS\u2010398\u2010sensitive manner suggesting that prostacyclin production derives principally by COX\u20102 pathway.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A novel NO\u2010releasing oxatriazole derivative GEA 3175 (1\u201330\u2003\u03bc<jats:sc>M<\/jats:sc>) inhibited LPS\u2010induced production of prostacyclin in HUVECs in a dose\u2010dependent manner being more potent than the earlier known NO\u2010donor S\u2010nitroso\u2010<jats:italic>N<\/jats:italic>\u2010acetylpenicillamine (SNAP).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The effects of the two NO\u2010donors on prostacyclin synthesis were reversed when red blood cells were added into the culture indicating that the effects are due to NO released from the compounds.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Addition of exogenous arachidonic acid into the culture did not alter the inhibitory action of NO\u2010donors suggesting that phospholipases are not the target of action of NO.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The NO\u2010donors did not inhibit prostacyclin production in the presence of a selective COX\u20102 inhibitor NS\u2010398. These data suggest that NO affects COX\u20102 pathway rather than has an overall effect on cyclooxygenases.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>NO\u2010releasing compounds did not alter the level of COX\u20102 protein expression in LPS\u2010treated HUVECs as measured by Western blot analysis.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results suggest that NO\u2010donors inhibit the activity of COX\u20102 in human endothelial cells. A link between NO and the regulation of eicosanoid synthesis could represent an important mechanism in controlling vascular and inflammatory responses in pathophysiological states and during treatment with nitrovasodilators.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (1998) <jats:bold>125<\/jats:bold>, 247\u2013254; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/sj.bjp.0702042\">10.1038\/sj.bjp.0702042<\/jats:ext-link><\/jats:p>","DOI":"10.1038\/sj.bjp.0702042","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T19:49:05Z","timestamp":1106941745000},"page":"247-254","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Inhibition by nitric oxide\u2010releasing compounds of prostacyclin production in human endothelial cells"],"prefix":"10.1111","volume":"125","author":[{"given":"Outi","family":"Kosonen","sequence":"first","affiliation":[]},{"given":"Hannu","family":"Kankaanranta","sequence":"additional","affiliation":[]},{"given":"Ulla","family":"Malo\u2010Ranta","sequence":"additional","affiliation":[]},{"given":"Ari","family":"Ristim\u00e4ki","sequence":"additional","affiliation":[]},{"given":"Eeva","family":"Moilanen","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119280"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1994.tb03855.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0518"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.2.274"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(91)90032-D"},{"key":"e_1_2_6_8_1","first-page":"123","article-title":"Biotransformation to nitric oxide of organic nitrates in comparison to other nitrovasodilators","volume":"14","author":"Feelisch M.","year":"1993","journal-title":"Eur. 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