{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:29:08Z","timestamp":1773707348678,"version":"3.50.1"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2007,10,19]],"date-time":"2007-10-19T00:00:00Z","timestamp":1192752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["www.embopress.org"],"crossmark-restriction":false},"short-container-title":["EMBO Reports"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:p>\n                    The quality control of proteins mediated by the plasticity of the proteasome system is regulated by the timely and flexible formation of this multisubunit proteolytic enzyme complex. Adaptable biogenesis of the 20S proteasome core complex is therefore of vital importance for adjusting to changing proteolytic requirements. However, the molecular mechanism and the cellular sites of mammalian proteasome formation are still unresolved. By using precursor complex\u2010specific antibodies, we now show that the main steps in 20S core complex formation take place at the endoplasmic reticulum (ER). Thereby, the proteasome maturation protein (POMP)\u2014an essential factor of mammalian proteasome biogenesis\u2014interacts with ER membranes, binds to \u03b1\n                    <jats:sub>1\u20137<\/jats:sub>\n                    rings, recruits \u03b2\u2010subunits stepwise and mediates the association of mammalian precursor complexes with the ER. Thus, POMP facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function.\n                  <\/jats:p>","DOI":"10.1038\/sj.embor.7401091","type":"journal-article","created":{"date-parts":[[2007,10,19]],"date-time":"2007-10-19T04:37:38Z","timestamp":1192768658000},"page":"1170-1175","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":102,"title":["The proteasome maturation protein POMP facilitates major steps of 20S proteasome formation at the endoplasmic reticulum"],"prefix":"10.1038","volume":"8","author":[{"given":"Benjamin","family":"Fricke","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Biochemie, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Monbijoustrasse 2  10117 Berlin Germany"}]},{"given":"Sylvia","family":"Heink","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr 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