{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T05:35:14Z","timestamp":1770960914383,"version":"3.50.1"},"reference-count":19,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["www.embopress.org"],"crossmark-restriction":false},"short-container-title":["EMBO Reports"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:p>\n                    Secretory and membrane N\u2010linked glycoproteins undergo folding and oligomeric assembly in the endoplasmic reticulum with the aid of a folding mechanism known as the calnexin cycle. UDP\u2013glucose glycoprotein:glucosyltransferase (UGGT) is the sensor component of the calnexin cycle, which recognizes these glycoproteins when they are incompletely folded, and transfers a glucose residue from UDP\u2013glucose to N\u2010linked Man9\u2010GlcNAc2 glycans. To determine how UGGT recognizes incompletely folded glycoproteins, we used purified enzyme to glucosylate a set of Man9\u2010GlcNAc2 glycopeptide substrates\n                    <jats:italic>in vitro<\/jats:italic>\n                    , and determined quantitatively the glucose incorporation into each glycan by mass spectrometry. A ranked order of glycopeptide specificity was found that provides the criteria for the recognition of substrates by UGGT. The preference for amino\u2010acid residues close to N\u2010linked glycans provides criteria for the recognition of glycopeptide substrates by UGGT.\n                  <\/jats:p>","DOI":"10.1038\/sj.embor.embor797","type":"journal-article","created":{"date-parts":[[2003,4,9]],"date-time":"2003-04-09T13:10:48Z","timestamp":1049893848000},"page":"405-411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Glycopeptide specificity of the secretory protein folding sensor UDP\u2013glucose glycoprotein:glucosyltransferase"],"prefix":"10.1038","volume":"4","author":[{"given":"Sean C","family":"Taylor","sequence":"first","affiliation":[{"name":"Biochemistry Department, Faculty of Medicine, McGill University, McIntyre Medical Sciences Building  3655 Promenade Sir William Osler Montreal Quebec Canada H3G 1Y6"},{"name":"Department of Anatomy and Cell Biology, McGill University  3640 University Street Montreal Quebec Canada H3A 2B2"}]},{"given":"Pierre","family":"Thibault","sequence":"additional","affiliation":[{"name":"Institute for Biological Sciences, National Research Council of Canada  100 Sussex Drive Ottawa Ontario Canada K1A 0R6"}]},{"given":"Daniel C","family":"Tessier","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Faculty of Medicine, McGill University, McIntyre Medical Sciences Building  3655 Promenade Sir William Osler Montreal Quebec Canada H3G 1Y6"}]},{"given":"John J M","family":"Bergeron","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology, McGill University  3640 University Street Montreal Quebec Canada H3A 2B2"}]},{"given":"David Y","family":"Thomas","sequence":"additional","affiliation":[{"name":"Biochemistry Department, Faculty of Medicine, McGill University, McIntyre Medical Sciences Building  3655 Promenade Sir William Osler Montreal Quebec Canada H3G 1Y6"},{"name":"Department of Anatomy and Cell Biology, McGill University  3640 University Street Montreal Quebec Canada H3A 2B2"}]}],"member":"297","published-online":{"date-parts":[[2003,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(94)90153-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)00937-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560020207"},{"key":"e_1_2_6_5_1","first-page":"75","article-title":"Hydrophobicity indices for amino\u2010acid residues as determined by high\u2010performance liquid chromatography","volume":"3","author":"Cowan R.","year":"1990","journal-title":"Peptide Res."},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2000.0759"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3824"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(86)90295-5"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19960610)10:8<889::AID-RCM615>3.0.CO;2-F"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.69"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.4.7.1238"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00416596"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/74035"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00318-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00093.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00116a015"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/10.4.403"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81855-3"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.11.6009"}],"container-title":["EMBO reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fsj.embor.embor797","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/sj.embor.embor797","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1038\/sj.embor.embor797","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.1038\/sj.embor.embor797","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T01:40:43Z","timestamp":1764985243000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1038\/sj.embor.embor797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1038\/sj.embor.embor797"],"URL":"https:\/\/doi.org\/10.1038\/sj.embor.embor797","archive":["Portico"],"relation":{},"ISSN":["1469-221X","1469-3178"],"issn-type":[{"value":"1469-221X","type":"print"},{"value":"1469-3178","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4]]},"assertion":[{"value":"2002-10-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-01-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}