{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:32:55Z","timestamp":1777530775587,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":212,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:sec><jats:title>Background and purpose:<\/jats:title><jats:p>Oncocalyxone A (OncoA) has a concentration\u2010dependent anti\u2010platelet activity. The present study aimed to further understand the mechanisms related to this effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental approach:<\/jats:title><jats:p>Human platelet aggregation was measured by means of a turbidimetric method. OncoA (32\u2013256\u2003\u03bc<jats:sc>M<\/jats:sc>) was tested against several platelet\u2010aggregating agents, such as adenosine diphosphate (ADP), collagen, arachidonic acid (AA), ristocetin and thrombin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key results:<\/jats:title><jats:p>OncoA completely inhibited platelet aggregation with a calculated mean inhibitory concentration (IC<jats:sub>50<\/jats:sub>\u2010\u03bc<jats:sc>M<\/jats:sc>) of 122 for ADP, 161 for collagen, 159 for AA, 169 for ristocetin and 85 for thrombin. The anti\u2010aggregatory activity of OncoA was not inhibited by 1H\u2010[1,2,4]oxadiazolo[4,3\u2010a]quinoxalin\u20101\u2010one (ODQ). OncoA, at a concentration that caused no significant anti\u2010aggregatory activity, potentiated sodium nitroprusside (SNP) anti\u2010aggregatory activity (18.8\u00b12.9%\u2010SNP vs 85.0\u00b18.2%\u2010SNP+OncoA). The levels of nitric oxide (NO) or cAMP were not altered by OncoA while cGMP levels were increased more than 10\u2010fold by OncoA in resting or ADP\u2010activated platelets. Flow cytometry revealed that OncoA does not interact with receptors for fibrinogen, collagen or P\u2010selectin. Nevertheless, OncoA decreased the binding of antibodies to GP Ib\u03b1, a glycoprotein that is related both to von Willebrand factor and to thrombin\u2010induced platelet aggregation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion and implications:<\/jats:title><jats:p>OncoA showed anti\u2010aggregatory activity in platelets that was associated with increased cGMP levels, not dependent on NO and with blocking GP Ib\u03b1 glycoprotein. This new mechanism has the prospect of leading to new anti\u2010thrombotic drugs.<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2008) <jats:bold>154<\/jats:bold>, 1216\u20131224; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/bjp.2008.199\">10.1038\/bjp.2008.199<\/jats:ext-link>; published online 2 June 2008<\/jats:p><\/jats:sec>","DOI":"10.1038\/bjp.2008.199","type":"journal-article","created":{"date-parts":[[2008,6,2]],"date-time":"2008-06-02T10:15:03Z","timestamp":1212401703000},"page":"1216-1224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Oncocalyxone A inhibits human platelet aggregation by increasing cGMP and by binding to GP Ib\u03b1 glycoprotein"],"prefix":"10.1111","volume":"154","author":[{"given":"M A D","family":"Ferreira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N R F","family":"Do Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C M","family":"De Sousa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O D L","family":"Pessoa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T L G","family":"De Lemos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J S","family":"Ventura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Schattner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A M","family":"Chudzinski\u2010Tavassi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1963.sp007185"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(91)90348-M"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01751.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(78)57003-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(84)90207-8"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000013777.21160.07"},{"key":"e_1_2_8_8_1","first-page":"183","article-title":"Antioxidant activity of Quinones isolated from Auxemma oncocalyx Taub","author":"Ferreira MAD","year":"2001","journal-title":"VI Pharmatech, Recife"},{"key":"e_1_2_8_9_1","first-page":"97","article-title":"Inhibition of platelet activation by quinones isolated from Auxemma oncocalyx Taub","volume":"106","author":"Ferreira MAD","year":"1999","journal-title":"Res Commun Mol Pathol Pharmacol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1078\/0944711041495227"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.26.595"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702495"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.10.5.865"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1995.tb05804.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(01)01631-9"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(02)03014-0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000072771.11429.83"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1615861"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198908243210804"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-3848(98)00100-5"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1538-7836.2003.00267.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.073718"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/62.1.176"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(95)00078-7"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"4226","DOI":"10.1182\/blood.V84.12.4226.bloodjournal84124226","article-title":"YC\u20101 a novel activator of platelet guanylate cyclase","volume":"84","author":"Ko FN","year":"1994","journal-title":"Blood"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0033-0620(03)00078-1"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612054367391"},{"key":"e_1_2_8_28_1","first-page":"1029","article-title":"Oncocalyxones A and C, 1,4\u2010anthracenediones from Auxemma oncocalyx: comparison with anticancer 1,9\u2010antracendiones","volume":"20","author":"Leyva A","year":"2000","journal-title":"Anticancer Res"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2002000800010"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)00325-3"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000130846.46168.03"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.4.1480"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000126286.47618.BD"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1573(200005)14:3<187::AID-PTR572>3.0.CO;2-I"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/tcm.10075"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(95)00397-P"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/10575639308043799"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-6865(99)00014-X"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1102-1227"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(01)00760-2"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2004.09.067"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(00)00686-X"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1989.69.1.58"},{"key":"e_1_2_8_44_1","first-page":"94","volume-title":"XXXIV Brazilian Meeting of the Pharmacology and Experimental Therapeutics Society","author":"Sousa CM","year":"2002"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/35065611"},{"key":"e_1_2_8_46_1","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1055\/s-0038-1649717","article-title":"Frangulin B, an antagonist of collagen\u2010induced platelet aggregation and adhesion, isolated from Rhamnus formosana","volume":"70","author":"Teng CM","year":"1993","journal-title":"Thromb Haemost"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1994.1106"},{"key":"e_1_2_8_48_1","doi-asserted-by":"crossref","first-page":"3C","DOI":"10.1016\/S0002-9149(99)00042-9","article-title":"Tissue distribution of phosphodiesterase families and effects of sildenafil on tissue cyclic nucleotides, platelet function, and the contractile responses of trabeculae carneae and aortic rings in vitro","volume":"83","author":"Wallis RM","year":"1999","journal-title":"Am J Cardiol"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi952456c"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(00)00225-X"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.24.618"}],"container-title":["British Journal of Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fbjp.2008.199","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Fbjp.2008.199","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/bjp.2008.199","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T15:25:27Z","timestamp":1697124327000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1038\/bjp.2008.199"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,7]]}},"alternative-id":["10.1038\/bjp.2008.199"],"URL":"https:\/\/doi.org\/10.1038\/bjp.2008.199","archive":["Portico"],"relation":{},"ISSN":["0007-1188","1476-5381"],"issn-type":[{"value":"0007-1188","type":"print"},{"value":"1476-5381","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7]]},"assertion":[{"value":"2009-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}