{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:53:42Z","timestamp":1789966422122,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":334,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bpspubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["British J Pharmacology"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:p>\n                    <jats:bold>Background and purpose:<\/jats:bold>\n                    When used to treat gouty arthritis, colchicine is believed to work by inhibiting microtubule\u2010dependent cell infiltration. However,\n                    <jats:italic>in vitro<\/jats:italic>\n                    , colchicine also reduces monosodium urate (MSU)\u2010induced superoxide production by neutrophils. Our study aimed to compare the effects of colchicine on neutrophil superoxide production and infiltration in an\n                    <jats:italic>in vivo<\/jats:italic>\n                    model of acute gouty inflammation.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Experimental approach:<\/jats:bold>\n                    <jats:italic>In vitro:<\/jats:italic>\n                    Human and murine peritoneal neutrophils were incubated with MSU with and without colchicine, and superoxide production was measured.\n                    <jats:italic>In vivo:<\/jats:italic>\n                    Mice were treated with colchicine followed by an intraperitoneal injection of MSU to induce acute inflammation. After 4h, the peritoneal cells were recovered to measure superoxide production and neutrophil infiltration. Sera were tested for liver and renal toxicity.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Key results:<\/jats:bold>\n                    Colchicine dose\u2010dependently inhibited MSU\u2010induced superoxide production by both human and murine neutrophils\n                    <jats:italic>in vitro.<\/jats:italic>\n                    Oral colchicine inhibited MSU\u2010induced superoxide production by neutrophils\n                    <jats:italic>in vivo<\/jats:italic>\n                    at doses 100 times lower than those required to inhibit neutrophil infiltration and without acute liver or renal toxicity. Neutrophils treated with colchicine\n                    <jats:italic>in vivo<\/jats:italic>\n                    still produced superoxide in response to another stimulus, 4\u2010\u03b2\u2010phorbol\u201012\u2010myristate\u201013\u2010acetate.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions and implications:<\/jats:bold>\n                    These results show a beneficial effect of colchicine for the treatment of MSU\u2010induced superoxide production\n                    <jats:italic>in vivo<\/jats:italic>\n                    at sub\u2010toxic doses without compromising superoxide production by other physiological processes. This is the first\n                    <jats:italic>in vivo<\/jats:italic>\n                    data to provide a biological rationale that supports the implementation of low dose, non\u2010toxic, colchicine therapy for the treatment of gouty arthritis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>British Journal of Pharmacology<\/jats:italic>\n                    (2008)\n                    <jats:bold>153<\/jats:bold>\n                    , 1288\u20131295; doi:\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/bjp.2008.20\">10.1038\/bjp.2008.20<\/jats:ext-link>\n                    ; published online 11 February 2008\n                  <\/jats:p>","DOI":"10.1038\/bjp.2008.20","type":"journal-article","created":{"date-parts":[[2008,2,11]],"date-time":"2008-02-11T05:59:26Z","timestamp":1202709566000},"page":"1288-1295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":93,"title":["Colchicine suppresses neutrophil superoxide production in a murine model of gouty arthritis: a rationale for use of low\u2010dose colchicine"],"prefix":"10.1111","volume":"153","author":[{"given":"E W","family":"Chia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Grainger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Harper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1445-5994.1987.tb01232.x"},{"key":"e_1_2_8_3_1","first-page":"552","article-title":"Studies with radioactive Colchicine. 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