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We investigated if a novel non\u2010antibacterial, non\u2010chelating hydroxypyrazoline derivative of minocycline, 12<jats:italic>S<\/jats:italic>\u2010hydroxy\u20101,12\u2010pyrazolinominocycline (PMIN), also induced antinociceptive and anti\u2010inflammatory effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental approach:<\/jats:title><jats:p>Antibacterial effects against a minocycline\u2010sensitive <jats:italic>Staphylococcus aureus<\/jats:italic> strain were evaluated by applying a cylinder\u2010plate agar diffusion technique. Antibacterial effects of diluted serum from mice pre\u2010treated with minocycline or PMIN were also evaluated. Ca<jats:sup>2+<\/jats:sup> binding activity was assessed by spectrophotometry. Formalin\u2010induced nociceptive responses and carrageenan\u2010induced paw oedema were evaluated in mice. The rota\u2010rod apparatus was used to evaluate motor coordination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Key results:<\/jats:title><jats:p>Minocycline, but not PMIN, inhibited bacterial growth. Serum from mice treated with minocycline, but not with PMIN, also induced such an effect. The UV absorption spectrum of solutions of minocycline, but not those of PMIN, was markedly changed in the presence of Ca<jats:sup>2+<\/jats:sup>. Minocycline or PMIN inhibited both phases of formalin\u2010induced nociception and carrageenan\u2010induced paw oedema. It is unlikely that antinociception resulted from lack of motor coordination, as tetracycline did not impair the performance of mice on the rotating rod.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions and implications:<\/jats:title><jats:p>These results indicate that inhibition of nociception and oedema by tetracyclines is neither necessarily linked to antibacterial nor to Ca<jats:sup>2+<\/jats:sup>chelating activities. This study supports the evaluation of the potential usefulness of PMIN in the treatment of painful and inflammatory diseases, as its lack of antibacterial and Ca<jats:sup>2+<\/jats:sup>chelating activities might confer greater safety over conventional tetracyclines.<\/jats:p><jats:p><jats:italic>British Journal of Pharmacology<\/jats:italic> (2008) <jats:bold>155<\/jats:bold>, 714\u2013721; doi:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1038\/bjp.2008.303\">10.1038\/bjp.2008.303<\/jats:ext-link>; published online 28 July 2008<\/jats:p><\/jats:sec>","DOI":"10.1038\/bjp.2008.303","type":"journal-article","created":{"date-parts":[[2008,7,28]],"date-time":"2008-07-28T10:09:42Z","timestamp":1217239782000},"page":"714-721","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A novel non\u2010antibacterial, non\u2010chelating hydroxypyrazoline derivative of 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