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This study used a three-dimensional (3D) flowing microfluidic chip to screen the effective candidate against MSU-stimulated human umbilical vein endothelial cell (HUVEC) damage, and found kinsenoside (Kin) to be the leading active component of<jats:italic>Anoectochilus roxburghi<\/jats:italic>, one of the Chinese medicinal plant widely used in the treatment of gouty arthritis clinically. Cell viability and apoptosis of HUVECs were evaluated, indicating that direct Kin stimulation and conditioned medium (CM) from Kin-treated macrophages both negatively modulated with MSU crystals. Additionally, Kin was capable of attenuating MSU-induced activation of nuclear factor-<jats:italic>\u03ba<\/jats:italic>B\/mitogen-activated protein kinase (NF-<jats:italic>\u03ba<\/jats:italic>B\/MAPK) signaling, targeting I<jats:italic>\u03ba<\/jats:italic>B kinase-<jats:italic>\u03b1<\/jats:italic>(IKK<jats:italic>\u03b1<\/jats:italic>) and IKK<jats:italic>\u03b2<\/jats:italic>kinases of macrophages and influencing the expressions of NF-<jats:italic>\u03ba<\/jats:italic>B downstream cytokines and subsequent HUVEC bioactivity. Inflammasome NLR pyrin domain-containing 3 (NALP3) and toll-like receptor 2 (TLR2) were also inhibited after Kin treatment. Also, Kin downregulated CD14-mediated MSU crystals uptake in macrophages.<jats:italic>In vivo<\/jats:italic>study with MSU-injected ankle joints further revealed the significant suppression of inflammatory infiltration and endothelia impairment coupled with alleviation of ankle swelling and nociceptive response via Kin treatments. Taken together, these data implicated that Kin was the most effective candidate from<jats:italic>Anoectochilus roxburghi<\/jats:italic>to treat gouty arthritis clinically.<\/jats:p>","DOI":"10.1038\/cddis.2016.255","type":"journal-article","created":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T12:54:31Z","timestamp":1472734471000},"page":"e2350-e2350","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Kinsenoside screening with a microfluidic chip attenuates gouty arthritis through inactivating NF-\u03baB signaling in macrophages and protecting endothelial cells"],"prefix":"10.1038","volume":"7","author":[{"given":"Qiao","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Bing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Di","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Shi-he","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Yu-hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Xiu-guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Yu-gang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Qi-ming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Shih-mo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tang","family":"Ting-ting","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,9,1]]},"reference":[{"key":"BFcddis2016255_CR1","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1189\/jlb.3MA0915-418R","volume":"99","author":"I Galvao","year":"2016","unstructured":"Galvao I, Dias AC, Tavares LD, Rodrigues IP, Queiroz-Junior CM, Costa VV et al. 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