{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T22:32:44Z","timestamp":1773095564797,"version":"3.50.1"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Death Dis"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Histone H3 (H3K4) demethylase JARID1B is aberrantly upregulated in many types of tumor and has been proposed to function as oncogene. Here we show that JARID1B is elevated in moderate and high-differentiated human hypopharyngeal squamous cell carcinoma (HPSCC) compared with low-differentiated HPSCC. Overexpression of JARID1B in FaDu cells increased epithelial differentiation marker K10 expression and inhibited cell proliferation. JARID1B and K10 mRNA expression is high correlated in HPSCC patients. Mechanistically, we found JARID1B directly bound to PI3K\/AKT signaling inhibitor SHIP1 gene promoter and decreased SHIP1 gene expression. Activation of downstream AKT resulted in increased <jats:italic>\u03b2<\/jats:italic>-catenin signaling, by which promoted target genes Fra-1 and Jun, together with other AP-1 transcription factors, leading to K10 expression. Forced expression of SHIP1 rescued JARID1B-induced phenotypes on FaDu cell differentiation and proliferation. Taken together, our findings provide first evidence that elevated expression of JARID1B has a critical role in promoting HPSCC differentiation and inhibiting proliferation, suggesting JARID1B may function as a tumor suppressor in squamous cell cancers and implying a novel important therapeutic strategy of HPSCC.<\/jats:p>","DOI":"10.1038\/cddis.2016.262","type":"journal-article","created":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T12:54:31Z","timestamp":1472734471000},"page":"e2358-e2358","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Overexpression of JARID1B promotes differentiation via SHIP1\/AKT signaling in human hypopharyngeal squamous cell carcinoma"],"prefix":"10.1038","volume":"7","author":[{"given":"Jisheng","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofei","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yafei","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingwei","family":"Chi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Llobet-Navas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,9,1]]},"reference":[{"key":"BFcddis2016262_CR1","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1002\/hed.21022","volume":"31","author":"JS Cooper","year":"2009","unstructured":"Cooper JS, Porter K, Mallin K, Hoffman HT, Weber RS, Ang KK et al. National cancer database report on cancer of the head and neck: 10-year update. Head Neck 2009; 31: 748\u2013758.","journal-title":"Head Neck"},{"key":"BFcddis2016262_CR2","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1002\/hed.21613","volume":"34","author":"RP Takes","year":"2012","unstructured":"Takes RP, Strojan P, Silver CE, Bradley PJ, Haigentz M Jr . Wolf GT et al. Current trends in initial management of hypopharyngeal cancer: The declining use of open surgery. Head Neck 2012; 34: 270\u2013281.","journal-title":"Head Neck"},{"key":"BFcddis2016262_CR3","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1097\/00005537-200205000-00011","volume":"112","author":"C Godballe","year":"2002","unstructured":"Godballe C, Jorgensen K, Hansen O, Bastholt L . Hypopharyngeal cancer: results of treatment based on radiation therapy and salvage surgery. Laryngoscope 2002; 112: 834\u2013838.","journal-title":"Laryngoscope"},{"key":"BFcddis2016262_CR4","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1067\/mhn.2001.111597","volume":"124","author":"W Steiner","year":"2001","unstructured":"Steiner W, Ambrosch P, Hess CF, Kron M . Organ preservation by transoral laser microsurgery in piriform sinus carcinoma. Otolaryngol Head Neck Surg 2001; 124: 58\u201367.","journal-title":"Otolaryngol Head Neck Surg"},{"key":"BFcddis2016262_CR5","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1097\/00005537-200106000-00028","volume":"111","author":"JG Spector","year":"2001","unstructured":"Spector JG, Sessions DG, Haughey BH, Chao KS, Simpson J, El Mofty S et al. Delayed regional metastases, distant metastases, and second primary malignancies in squamous cell carcinomas of the larynx and hypopharynx. Laryngoscope 2001; 111: 1079\u20131087.","journal-title":"Laryngoscope"},{"key":"BFcddis2016262_CR6","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1093\/jnci\/88.13.890","volume":"88","author":"JL Lefebvre","year":"1996","unstructured":"Lefebvre JL, Chevalier D, Luboinski B, Kirkpatrick A, Collette L, Sahmoud T . Larynx preservation in pyriform sinus cancer: Preliminary results of a european organization for research and treatment of cancer phase iii trial. Eortc head and neck cancer cooperative group. J Natl Cancer Inst 1996; 88: 890\u2013899.","journal-title":"J Natl Cancer Inst"},{"key":"BFcddis2016262_CR7","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1002\/ijc.10644","volume":"101","author":"A Barrett","year":"2002","unstructured":"Barrett A, Madsen B, Copier J, Lu PJ, Cooper L, Scibetta AG et al. Plu-1 nuclear protein, which is upregulated in breast cancer, shows restricted expression in normal human adult tissues: a new cancer\/testis antigen? Int J Cancer 2002; 101: 581\u2013588.","journal-title":"Int J Cancer"},{"key":"BFcddis2016262_CR8","doi-asserted-by":"publisher","first-page":"15633","DOI":"10.1074\/jbc.274.22.15633","volume":"274","author":"PJ Lu","year":"1999","unstructured":"Lu PJ, Sundquist K, Baeckstrom D, Poulsom R, Hanby A, Meier-Ewert S et al. A novel gene (plu-1) containing highly conserved putative DNA\/chromatin binding motifs is specifically up-regulated in breast cancer. J Biol Chem 1999; 274: 15633\u201315645.","journal-title":"J Biol Chem"},{"key":"BFcddis2016262_CR9","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1038\/nature11412","volume":"490","author":"Cancer Genome Atlas N","year":"2012","unstructured":"Cancer Genome Atlas N. Comprehensive molecular portraits of human breast tumours. Nature 2012; 490: 61\u201370.","journal-title":"Nature"},{"key":"BFcddis2016262_CR10","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1186\/1476-4598-9-59","volume":"9","author":"S Hayami","year":"2010","unstructured":"Hayami S, Yoshimatsu M, Veerakumarasivam A, Unoki M, Iwai Y, Tsunoda T et al. Overexpression of the jmjc histone demethylase kdm5b in human carcinogenesis: Involvement in the proliferation of cancer cells through the e2f\/rb pathway. Mole Cancer 2010; 9: 59.","journal-title":"Mole Cancer"},{"key":"BFcddis2016262_CR11","doi-asserted-by":"publisher","first-page":"7866","DOI":"10.3390\/ijms14047866","volume":"14","author":"X Li","year":"2013","unstructured":"Li X, Su Y, Pan J, Zhou Z, Song B, Xiong E et al. Connexin 26 is down-regulated by kdm5b in the progression of bladder cancer. Int J Mol Sci 2013; 14: 7866\u20137879.","journal-title":"Int J Mol Sci"},{"key":"BFcddis2016262_CR12","doi-asserted-by":"publisher","first-page":"1212","DOI":"10.3892\/ijo.2013.1799","volume":"42","author":"K Ohta","year":"2013","unstructured":"Ohta K, Haraguchi N, Kano Y, Kagawa Y, Konno M, Nishikawa S et al. Depletion of jarid1b induces cellular senescence in human colorectal cancer. Int J Oncol 2013; 42: 1212\u20131218.","journal-title":"Int J Oncol"},{"key":"BFcddis2016262_CR13","doi-asserted-by":"publisher","first-page":"19226","DOI":"10.1073\/pnas.0700735104","volume":"104","author":"Y Xiang","year":"2007","unstructured":"Xiang Y, Zhu Z, Han G, Ye X, Xu B, Peng Z et al. Jarid1b is a histone h3 lysine 4 demethylase up-regulated in prostate cancer. Proc Nat Acad Sci USA 2007; 104: 19226\u201319231.","journal-title":"Proc Nat Acad Sci USA"},{"key":"BFcddis2016262_CR14","doi-asserted-by":"publisher","first-page":"811","DOI":"10.1016\/j.ccr.2013.05.003","volume":"23","author":"A Roesch","year":"2013","unstructured":"Roesch A, Vultur A, Bogeski I, Wang H, Zimmermann KM, Speicher D et al. Overcoming intrinsic multidrug resistance in melanoma by blocking the mitochondrial respiratory chain of slow-cycling jarid1b(high) cells. Cancer Cell 2013; 23: 811\u2013825.","journal-title":"Cancer Cell"},{"key":"BFcddis2016262_CR15","first-page":"1267","volume":"38","author":"S Catchpole","year":"2011","unstructured":"Catchpole S, Spencer-Dene B, Hall D, Santangelo S, Rosewell I, Guenatri M et al. Plu-1\/jarid1b\/kdm5b is required for embryonic survival and contributes to cell proliferation in the mammary gland and in er+ breast cancer cells. Int J Oncol 2011; 38: 1267\u20131277.","journal-title":"Int J Oncol"},{"key":"BFcddis2016262_CR16","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/j.molcel.2007.03.001","volume":"25","author":"K Yamane","year":"2007","unstructured":"Yamane K, Tateishi K, Klose RJ, Fang J, Fabrizio LA, Erdjument-Bromage H et al. Plu-1 is an h3k4 demethylase involved in transcriptional repression and breast cancer cell proliferation. Mol Cell 2007; 25: 801\u2013812.","journal-title":"Mol Cell"},{"key":"BFcddis2016262_CR17","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1016\/j.cell.2010.04.020","volume":"141","author":"A Roesch","year":"2010","unstructured":"Roesch A, Fukunaga-Kalabis M, Schmidt EC, Zabierowski SE, Brafford PA, Vultur A et al. A temporarily distinct subpopulation of slow-cycling melanoma cells is required for continuous tumor growth. Cell 2010; 141: 583\u2013594.","journal-title":"Cell"},{"key":"BFcddis2016262_CR18","doi-asserted-by":"publisher","first-page":"28329","DOI":"10.1074\/jbc.M115.657825","volume":"290","author":"A Rojas","year":"2015","unstructured":"Rojas A, Aguilar R, Henriquez B, Lian JB, Stein JL, Stein GS et al. Epigenetic control of the bone-master runx2 gene during osteoblast-lineage commitment by the histone demethylase jarid1b\/kdm5b. J Biol Chem 2015; 290: 28329\u201328342.","journal-title":"J Biol Chem"},{"key":"BFcddis2016262_CR19","doi-asserted-by":"publisher","first-page":"4450","DOI":"10.1038\/emboj.2011.317","volume":"30","author":"V Pospisil","year":"2011","unstructured":"Pospisil V, Vargova K, Kokavec J, Rybarova J, Savvulidi F, Jonasova A et al. Epigenetic silencing of the oncogenic mir-17-92 cluster during pu.1-directed macrophage differentiation. EMBO J 2011; 30: 4450\u20134464.","journal-title":"EMBO J"},{"key":"BFcddis2016262_CR20","doi-asserted-by":"publisher","first-page":"4586","DOI":"10.1038\/emboj.2011.383","volume":"30","author":"SU Schmitz","year":"2011","unstructured":"Schmitz SU, Albert M, Malatesta M, Morey L, Johansen JV, Bak M et al. Jarid1b targets genes regulating development and is involved in neural differentiation. EMBO J 2011; 30: 4586\u20134600.","journal-title":"EMBO J"},{"key":"BFcddis2016262_CR21","doi-asserted-by":"publisher","first-page":"4793","DOI":"10.1128\/MCB.00692-13","volume":"33","author":"BL Kidder","year":"2013","unstructured":"Kidder BL, Hu G, Yu ZX, Liu C, Zhao K . Extended self-renewal and accelerated reprogramming in the absence of kdm5b. Mol Cell Biol 2013; 33: 4793\u20134810.","journal-title":"Mol Cell Biol"},{"key":"BFcddis2016262_CR22","doi-asserted-by":"publisher","first-page":"762","DOI":"10.1016\/j.ccr.2014.04.024","volume":"25","author":"S Yamamoto","year":"2014","unstructured":"Yamamoto S, Wu Z, Russnes HG, Takagi S, Peluffo G, Vaske C et al. Jarid1b is a luminal lineage-driving oncogene in breast cancer. Cancer Cell 2014; 25: 762\u2013777.","journal-title":"Cancer Cell"},{"key":"BFcddis2016262_CR23","doi-asserted-by":"publisher","first-page":"1603","DOI":"10.1073\/pnas.96.4.1603","volume":"96","author":"B Mann","year":"1999","unstructured":"Mann B, Gelos M, Siedow A, Hanski ML, Gratchev A, Ilyas M et al. Target genes of beta-catenin-t cell-factor\/lymphoid-enhancer-factor signaling in human colorectal carcinomas. Proc Natl Acad Sci USA 1999; 96: 1603\u20131608.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFcddis2016262_CR24","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1101\/gad.249748.114","volume":"29","author":"S Wurm","year":"2015","unstructured":"Wurm S, Zhang J, Guinea-Viniegra J, Garcia F, Munoz J, Bakiri L et al. Terminal epidermal differentiation is regulated by the interaction of fra-2\/ap-1 with ezh2 and erk1\/2. Genes Dev 2015; 29: 144\u2013156.","journal-title":"Genes Dev"},{"key":"BFcddis2016262_CR25","doi-asserted-by":"publisher","first-page":"1122","DOI":"10.1016\/j.cell.2008.12.043","volume":"136","author":"E Ezhkova","year":"2009","unstructured":"Ezhkova E, Pasolli HA, Parker JS, Stokes N, Su IH, Hannon G et al. Ezh2 orchestrates gene expression for the stepwise differentiation of tissue-specific stem cells. Cell 2009; 136: 1122\u20131135.","journal-title":"Cell"},{"key":"BFcddis2016262_CR26","doi-asserted-by":"publisher","first-page":"11221","DOI":"10.1074\/jbc.M611871200","volume":"282","author":"D Fang","year":"2007","unstructured":"Fang D, Hawke D, Zheng Y, Xia Y, Meisenhelder J, Nika H et al. Phosphorylation of beta-catenin by akt promotes beta-catenin transcriptional activity. J Biol Chem 2007; 282: 11221\u201311229.","journal-title":"J Biol Chem"},{"key":"BFcddis2016262_CR27","doi-asserted-by":"publisher","first-page":"9971","DOI":"10.1074\/jbc.M508778200","volume":"281","author":"S Taurin","year":"2006","unstructured":"Taurin S, Sandbo N, Qin Y, Browning D, Dulin NO . Phosphorylation of beta-catenin by cyclic amp-dependent protein kinase. J Biol Chem 2006; 281: 9971\u20139976.","journal-title":"J Biol Chem"},{"key":"BFcddis2016262_CR28","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1038\/ng1928","volume":"39","author":"XC He","year":"2007","unstructured":"He XC, Yin T, Grindley JC, Tian Q, Sato T, Tao WA et al. Pten-deficient intestinal stem cells initiate intestinal polyposis. Nat Genet 2007; 39: 189\u2013198.","journal-title":"Nat Genet"},{"key":"BFcddis2016262_CR29","doi-asserted-by":"publisher","first-page":"2161","DOI":"10.1007\/s00018-012-0920-x","volume":"69","author":"J Zhang","year":"2012","unstructured":"Zhang J, Bardot E, Ezhkova E . Epigenetic regulation of skin: Focus on the polycomb complex. Cell Mol Life Sci 2012; 69: 2161\u20132172.","journal-title":"Cell Mol Life Sci"},{"key":"BFcddis2016262_CR30","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1038\/cr.2011.24","volume":"21","author":"HC Tsai","year":"2011","unstructured":"Tsai HC, Baylin SB . Cancer epigenetics: linking basic biology to clinical medicine. Cell Res 2011; 21: 502\u2013517.","journal-title":"Cell Res"},{"key":"BFcddis2016262_CR31","doi-asserted-by":"publisher","first-page":"1990","DOI":"10.1038\/emboj.2013.110","volume":"32","author":"ES Bardot","year":"2013","unstructured":"Bardot ES, Valdes VJ, Zhang J, Perdigoto CN, Nicolis S, Hearn SA et al. Polycomb subunits ezh1 and ezh2 regulate the merkel cell differentiation program in skin stem cells. EMBO J 2013; 32: 1990\u20132000.","journal-title":"EMBO J"},{"key":"BFcddis2016262_CR32","doi-asserted-by":"publisher","first-page":"e69784","DOI":"10.1371\/journal.pone.0069784","volume":"8","author":"X Liang","year":"2013","unstructured":"Liang X, Zeng J, Wang L, Fang M, Wang Q, Zhao M et al. Histone demethylase retinoblastoma binding protein 2 is overexpressed in hepatocellular carcinoma and negatively regulated by hsa-mir-212. PloS one 2013; 8: e69784.","journal-title":"PloS one"},{"key":"BFcddis2016262_CR33","doi-asserted-by":"publisher","first-page":"2666","DOI":"10.1002\/jcb.24613","volume":"114","author":"Z Jiping","year":"2013","unstructured":"Jiping Z, Ming F, Lixiang W, Xiuming L, Yuqun S, Han Y et al. Microrna-212 inhibits proliferation of gastric cancer by directly repressing retinoblastoma binding protein 2. J Cell Biochem 2013; 114: 2666\u20132672.","journal-title":"J Cell Biochem"},{"key":"BFcddis2016262_CR34","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1053\/j.gastro.2009.10.004","volume":"138","author":"J Zeng","year":"2010","unstructured":"Zeng J, Ge Z, Wang L, Li Q, Wang N, Bjorkholm M et al. The histone demethylase rbp2 is overexpressed in gastric cancer and its inhibition triggers senescence of cancer cells. Gastroenterology 2010; 138: 981\u2013992.","journal-title":"Gastroenterology"},{"key":"BFcddis2016262_CR35","doi-asserted-by":"publisher","first-page":"4711","DOI":"10.1158\/0008-5472.CAN-12-3165","volume":"73","author":"YC Teng","year":"2013","unstructured":"Teng YC, Lee CF, Li YS, Chen YR, Hsiao PW, Chan MY et al. Histone demethylase rbp2 promotes lung tumorigenesis and cancer metastasis. Cancer Res 2013; 73: 4711\u20134721.","journal-title":"Cancer Res"},{"key":"BFcddis2016262_CR36","doi-asserted-by":"publisher","first-page":"1633","DOI":"10.1128\/MCB.06373-11","volume":"32","author":"PP Wong","year":"2012","unstructured":"Wong PP, Miranda F, Chan KV, Berlato C, Hurst HC, Scibetta AG . Histone demethylase kdm5b collaborates with tfap2c and myc to repress the cell cycle inhibitor p21(cip) (cdkn1a). Mol Cell Biol 2012; 32: 1633\u20131644.","journal-title":"Mol Cell Biol"},{"key":"BFcddis2016262_CR37","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1038\/onc.2011.266","volume":"31","author":"X Niu","year":"2012","unstructured":"Niu X, Zhang T, Liao L, Zhou L, Lindner DJ, Zhou M et al. The von hippel-lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase jarid1c. Oncogene 2012; 31: 776\u2013786.","journal-title":"Oncogene"},{"key":"BFcddis2016262_CR38","doi-asserted-by":"publisher","first-page":"8971","DOI":"10.1073\/pnas.1119836109","volume":"109","author":"A Chicas","year":"2012","unstructured":"Chicas A, Kapoor A, Wang X, Aksoy O, Evertts AG, Zhang MQ et al. H3k4 demethylation by jarid1a and jarid1b contributes to retinoblastoma-mediated gene silencing during cellular senescence. Proc Natl Acad Sci USA 2012; 109: 8971\u20138976.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFcddis2016262_CR39","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1158\/0008-5472.CAN-15-0906","volume":"76","author":"N Li","year":"2016","unstructured":"Li N, Dhar SS, Chen TY, Kan PY, Wei Y, Kim JH et al. Jarid1d is a suppressor and prognostic marker of prostate cancer invasion and metastasis. Cancer Res 2016; 76: 831\u2013843.","journal-title":"Cancer Res"},{"key":"BFcddis2016262_CR40","doi-asserted-by":"publisher","first-page":"8638","DOI":"10.1073\/pnas.1500722112","volume":"112","author":"MC Caino","year":"2015","unstructured":"Caino MC, Ghosh JC, Chae YC, Vaira V, Rivadeneira DB, Faversani A et al. Pi3k therapy reprograms mitochondrial trafficking to fuel tumor cell invasion. Proc Natl Acad Sci USA 2015; 112: 8638\u20138643.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFcddis2016262_CR41","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/j.mce.2015.12.018","volume":"422","author":"S Zhang","year":"2015","unstructured":"Zhang S, Chen X, Hu Y, Wu J, Cao Q, Chen S et al. All-trans retinoic acid modulates wnt3a-induced osteogenic differentiation of mesenchymal stem cells via activating the pi3k\/akt\/gsk3beta signalling pathway. Mol Cell Endocrinol 2015; 422: 243\u2013253.","journal-title":"Mol Cell Endocrinol"},{"key":"BFcddis2016262_CR42","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1038\/nprot.2006.98","volume":"1","author":"TI Lee","year":"2006","unstructured":"Lee TI, Johnstone SE, Young RA . Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc 2006; 1: 729\u2013748.","journal-title":"Nat Protoc"}],"container-title":["Cell Death &amp; Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/cddis2016262.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/cddis2016262","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/cddis2016262.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T09:06:38Z","timestamp":1672823198000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/cddis2016262"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,1]]},"references-count":42,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2016,9]]}},"alternative-id":["BFcddis2016262"],"URL":"https:\/\/doi.org\/10.1038\/cddis.2016.262","relation":{},"ISSN":["2041-4889"],"issn-type":[{"value":"2041-4889","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,1]]},"assertion":[{"value":"21 March 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 June 2016","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 July 2016","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 September 2016","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}