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Here, we demonstrate a loss of marginal zone B lymphocytes (MZ, CD19<jats:sup>+<\/jats:sup>CD45R<jats:sup>+<\/jats:sup>CD21<jats:sup>++<\/jats:sup>CD23<jats:sup>lo<\/jats:sup>) and a decrease of naive B cells (CD19<jats:sup>+<\/jats:sup>IgD<jats:sup>+<\/jats:sup>), whereas there is an enhancement of a CD19<jats:sup>+<\/jats:sup>CD45R<jats:sup>lo<\/jats:sup>innate-like B cell population (B1REL) and the so-called aged B cell compartment (ABC, CD45R<jats:sup>+<\/jats:sup>CD21<jats:sup>lo<\/jats:sup>CD23<jats:sup>lo<\/jats:sup>CD5<jats:sup>\u2212<\/jats:sup>CD11b<jats:sup>\u2212<\/jats:sup>) in aged senescence-accelerated (SAMP8) mice but not in aged senescence-resistant (SAMR1) mice. These changes in aged SAMP8 mice were associated with lower IgG isotype levels, displaying low variable gene usage repertoires of the immunoglobulin heavy chain (V<jats:sub>H<\/jats:sub>) diversity, with a diminution on IgG1-memory B cells (CD11b<jats:sup>\u2212<\/jats:sup>Gr1<jats:sup>\u2212<\/jats:sup>CD138<jats:sup>\u2212<\/jats:sup>IgM<jats:sup>\u2212<\/jats:sup>IgD<jats:sup>\u2212<\/jats:sup>CD19<jats:sup>+<\/jats:sup>CD38<jats:sup>+<\/jats:sup>IgG1<jats:sup>+<\/jats:sup>), an increase in T follicular helper (T<jats:sub>FH<\/jats:sub>, CD4<jats:sup>+<\/jats:sup>CXCR5<jats:sup>+<\/jats:sup>PD1<jats:sup>+<\/jats:sup>) cell numbers, and an altered MOMA-1 (metallophilic macrophages) band in primary follicles. LPS-mediated IgG1 responses were impaired in the B1REL and ABC cell compartments, both<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>. These data demonstrate the prominent changes to different B cell populations and in structural follicle organization that occur upon aging in SAMP8 mice. These novel results raise new questions regarding the importance of the cellular distribution in the B cell layers, and their effector functions needed to mount a coordinated and effective humoral response.<\/jats:p>","DOI":"10.1038\/cddis.2017.351","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T12:41:06Z","timestamp":1502973666000},"page":"e3000-e3000","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Altered marginal zone and innate-like B cells in aged senescence-accelerated SAMP8 mice with defective IgG1 responses"],"prefix":"10.1038","volume":"8","author":[{"given":"Isabel","family":"Cortegano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Mart\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Carmen","family":"Prado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Ru\u00edz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Hortig\u00fcela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Al\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00e7al","family":"Vilar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Mira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Cano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercedes","family":"Dom\u00ednguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bel\u00e9n","family":"de Andr\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Luisa","family":"Gaspar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,8,17]]},"reference":[{"key":"BFcddis2017351_CR1","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1111\/j.0105-2896.2005.00271.x","volume":"205","author":"G Pawelec","year":"2005","unstructured":"Pawelec G, Akbar A, Caruso C, Solana R, Grubeck-Loebenstein B, Wikby A . 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