{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T06:09:24Z","timestamp":1759730964270},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2010,7,26]],"date-time":"2010-07-26T00:00:00Z","timestamp":1280102400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2010,7,26]],"date-time":"2010-07-26T00:00:00Z","timestamp":1280102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Mol Immunol"],"published-print":{"date-parts":[[2010,9]]},"DOI":"10.1038\/cmi.2010.33","type":"journal-article","created":{"date-parts":[[2010,7,26]],"date-time":"2010-07-26T08:39:37Z","timestamp":1280133577000},"page":"349-354","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Identification of two aberrant transcripts derived from a hybridoma with amplification of functional immunoglobulin variable genes"],"prefix":"10.1038","volume":"7","author":[{"given":"Guipeng","family":"Ding","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ximin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2010,7,26]]},"reference":[{"key":"BFcmi201033_CR1","doi-asserted-by":"publisher","first-page":"1915","DOI":"10.1158\/1078-0432.CCR-09-2425","volume":"16","author":"B Kuenen","year":"2010","unstructured":"Kuenen B, Witteveen PO, Ruijter R, Giaccone G, Dontabhaktuni A, Fox F et al. A phase I pharmacologic study of necitumumab (IMC-11F8), a fully human IgG1 monoclonal antibody directed against EGFR in patients with advanced solid malignancies. Clin Cancer Res 2010; 16: 1915\u20131923.","journal-title":"Clin Cancer Res"},{"key":"BFcmi201033_CR2","doi-asserted-by":"publisher","first-page":"1451","DOI":"10.1007\/s00262-006-0148-4","volume":"55","author":"I Azinovic","year":"2006","unstructured":"Azinovic I, DeNardo GL, Lamborn KR, Mirick G, Goldstein D, Bradt BM et al. Survival benefit associated with human anti-mouse antibody (HAMA) in patients with B-cell malignancies. Cancer Immunol Immunother 2006; 55: 1451\u20131458.","journal-title":"Cancer Immunol Immunother"},{"key":"BFcmi201033_CR3","doi-asserted-by":"publisher","first-page":"3902","DOI":"10.1128\/IAI.00200-09","volume":"77","author":"Z Chen","year":"2009","unstructured":"Chen Z, Moayeri M, Crown D, Emerson S, Gorshkova I, Schuck P et al. Novel chimpanzee\/human monoclonal antibodies that neutralize anthrax lethal factor, and evidence for possible synergy with anti-protective antigen antibody. Infect Immun 2009; 77: 3902\u20133908.","journal-title":"Infect Immun"},{"key":"BFcmi201033_CR4","doi-asserted-by":"publisher","first-page":"1933","DOI":"10.1158\/0008-5472.CAN-08-2707","volume":"69","author":"CJ Riley","year":"2009","unstructured":"Riley CJ, Engelhardt KP, Saldanha JW, Qi W, Cooke LS, Zhu Y et al. Design and activity of a murine and humanized anti-CEACAM6 single-chain variable fragment in the treatment of pancreatic cancer. Cancer Res 2009; 69: 1933\u20131940.","journal-title":"Cancer Res"},{"key":"BFcmi201033_CR5","doi-asserted-by":"publisher","first-page":"7880","DOI":"10.1074\/jbc.M109.061457","volume":"285","author":"YJ Huang","year":"2010","unstructured":"Huang YJ, Chen IC, Yu CM, Lee YC, Hsu HJ, Ching AT et al. Engineering anti-vascular endothelial growth factor single chain disulfide-stabilized antibody variable fragments (sc-dsFv) with phage-displayed sc-dsFv libraries. J Biol Chem 2010; 285: 7880\u20137891.","journal-title":"J Biol Chem"},{"key":"BFcmi201033_CR6","doi-asserted-by":"publisher","first-page":"2858","DOI":"10.1002\/pmic.200700968","volume":"8","author":"J Stadlmann","year":"2008","unstructured":"Stadlmann J, Pabst M, Kolarich D, Kunert R, Altmann F . Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides. Proteomics 2008; 8: 2858\u20132871.","journal-title":"Proteomics"},{"key":"BFcmi201033_CR7","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1016\/0161-5890(88)90005-3","volume":"25","author":"WL Carroll","year":"1988","unstructured":"Carroll WL, Mendel E, Levy S . Hybridoma fusion cell lines contain an aberrant kappa transcript. Mol Immunol 1988; 25: 991\u2013995.","journal-title":"Mol Immunol"},{"key":"BFcmi201033_CR8","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.1016\/j.molimm.2003.11.037","volume":"40","author":"TC Laurent","year":"2004","unstructured":"Laurent TC, Mertens P, Dierick JF, Letesson JJ, Lambert C, de Bolle X . Functional, molecular and structural characterisation of five anti-Brucella LPS mAb. Mol Immunol 2004; 40: 1237\u20131247.","journal-title":"Mol Immunol"},{"key":"BFcmi201033_CR9","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/0022-1759(93)90109-K","volume":"165","author":"PJ Nicholls","year":"1993","unstructured":"Nicholls PJ, Johnson VG, Blanford MD, Andrew SM . An improved method for generating single-chain antibodies from hybridomas. J Immunol Methods 1993; 165: 81\u201391.","journal-title":"J Immunol Methods"},{"key":"BFcmi201033_CR10","doi-asserted-by":"publisher","first-page":"818","DOI":"10.2144\/99265bm04","volume":"26","author":"O Cochet","year":"1999","unstructured":"Cochet O, Martin E, Fridman WH, Teillaud JL . Selective PCR amplification of functional immunoglobulin light chain from hybridoma containing the aberrant MOPC 21-derived V kappa by PNA-mediated PCR clamping. Biotechniques 1999; 26: 818\u2013820.","journal-title":"Biotechniques"},{"key":"BFcmi201033_CR11","doi-asserted-by":"publisher","first-page":"1979","DOI":"10.1093\/nar\/24.10.1979","volume":"24","author":"C Ostermeier","year":"1996","unstructured":"Ostermeier C, Michel H . Improved cloning of antibody variable regions from hybridomas by an antisense-directed RNase H digestion of the P3-X63-Ag8.653 derived pseudogene mRNA. Nucleic Acids Res 1996; 24: 1979\u20131980.","journal-title":"Nucleic Acids Res"},{"key":"BFcmi201033_CR12","doi-asserted-by":"publisher","first-page":"5433","DOI":"10.1093\/nar\/22.24.5433","volume":"22","author":"L Duan","year":"1994","unstructured":"Duan L, Pomerantz RJ . Elimination of endogenous aberrant kappa chain transcripts from sp2\/0-derived hybridoma cells by specific ribozyme cleavage: utility in genetic therapy of HIV-1 infections. Nucleic Acids Res 1994; 22: 5433\u20135438.","journal-title":"Nucleic Acids Res"},{"key":"BFcmi201033_CR13","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.ab.2005.10.046","volume":"349","author":"M Juste","year":"2006","unstructured":"Juste M, Muzard J, Billiald P . Cloning of the antibody kappa light chain V-gene from murine hybridomas by bypassing the aberrant MOPC21-derived transcript. Anal Biochem 2006; 349: 159\u2013161.","journal-title":"Anal Biochem"},{"key":"BFcmi201033_CR14","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.jim.2008.04.014","volume":"336","author":"Y Irani","year":"2008","unstructured":"Irani Y, Tea M, Tilton RG, Coster DJ, Williams KA, Brereton HM . PCR amplification of the functional immunoglobulin heavy chain variable gene from a hybridoma in the presence of two aberrant transcripts. J Immunol Methods 2008; 336: 246\u2013250.","journal-title":"J Immunol Methods"},{"key":"BFcmi201033_CR15","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1089\/hyb.1992.11.23","volume":"11","author":"G K\u00fctemeier","year":"1992","unstructured":"K\u00fctemeier G, Harloff C, Mocikat R . Rapid isolation of immunoglobulin variable genes from cell lysates of rat hybridomas by polymerase chain reaction. Hybridoma 1992; 11: 23\u201332.","journal-title":"Hybridoma"},{"key":"BFcmi201033_CR16","doi-asserted-by":"publisher","first-page":"2454","DOI":"10.1073\/pnas.84.8.2454","volume":"84","author":"AM Lawler","year":"1987","unstructured":"Lawler AM, Lin PS, Gearhart PJ . Adult B-cell repertoire is biased toward two heavy-chain variable-region genes that rearrange frequently in fetal pre-B cells. Proc Natl Acad Sci USA 1987; 84: 2454\u20132458.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFcmi201033_CR17","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/S0022-1759(96)00208-6","volume":"201","author":"A Krebber","year":"1997","unstructured":"Krebber A, Bornhauser S, Burmester J, Honegger A, Willuda J, Bosshard HR et al. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J Immunol Methods 1997; 201: 35\u201355.","journal-title":"J Immunol Methods"},{"key":"BFcmi201033_CR18","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/S0022-1759(00)00337-9","volume":"249","author":"G Vidarsson","year":"2001","unstructured":"Vidarsson G, van de Winkel JG, van Dijk MA . Multiplex screening for functionally rearranged immunoglobulin variable regions reveals expression of hybridoma-specific aberrant V-genes. J Immunol Methods 2001; 249: 245\u2013252.","journal-title":"J Immunol Methods"},{"key":"BFcmi201033_CR19","doi-asserted-by":"publisher","first-page":"129","DOI":"10.3233\/HAB-2003-12404","volume":"12","author":"P Zhao","year":"2003","unstructured":"Zhao P, Liang X, Kalbfleisch J, Koo HM, Cao B . Neutralizing monoclonal antibody against anthrax lethal factor inhibits intoxication in a mouse model. Hum Antibodies 2003; 12: 129\u2013135.","journal-title":"Hum Antibodies"},{"key":"BFcmi201033_CR20","first-page":"9.37","volume-title":"Phage Display: A Laboratory Manual","author":"J Andris-Widhopf","year":"2001","unstructured":"Andris-Widhopf J, Steinberger P, Fuller R, Rader C, Barbas CF . Generation of antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences. In: Barbas CF, Burton DR, Scott JK, Silverman GJ (eds.) Phage Display: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 2001: 9.37\u20139.52."},{"key":"BFcmi201033_CR21","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/0022-1759(92)90092-8","volume":"152","author":"MJ Coloma","year":"1992","unstructured":"Coloma MJ, Hastings A, Wims LA, Morrison SL . Novel vectors for the expression of antibody molecules using variable regions generated by polymerase chain reaction. J Immunol Methods 1992; 152: 89\u2013104.","journal-title":"J Immunol Methods"},{"key":"BFcmi201033_CR22","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1089\/153685903322328974","volume":"22","author":"L Roque-Navarro","year":"2003","unstructured":"Roque-Navarro L, Mateo C, Lombardero J, Mustelier G, Fern\u00e1ndez A, Sosa K et al. Humanization of predicted T-cell epitopes reduces the immunogenicity of chimeric antibodies: new evidence supporting a simple method. Hybrid Hybridomics 2003; 22: 245\u2013257.","journal-title":"Hybrid Hybridomics"},{"key":"BFcmi201033_CR23","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1007\/s10616-007-9115-y","volume":"57","author":"T Sugahara","year":"2008","unstructured":"Sugahara T, Nishimoto S, Miyazaki Y . Effects of polyamines on proliferation and IgM productivity of human\u2013human hybridoma, HB4C5 cells. Cytotechnology 2008; 57: 115\u2013122.","journal-title":"Cytotechnology"},{"key":"BFcmi201033_CR24","doi-asserted-by":"publisher","first-page":"13487","DOI":"10.1073\/pnas.0906581106","volume":"106","author":"Z Chen","year":"2009","unstructured":"Chen Z, Moayeri M, Zhao H, Crown D, Leppla SH, Purcell RH . Potent neutralization of anthrax edema toxin by a humanized monoclonal antibody that competes with calmodulin for edema factor binding. Proc Natl Acad Sci USA 2009; 106: 13487\u201313492.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFcmi201033_CR25","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.jim.2008.09.017","volume":"339","author":"S Rohatgi","year":"2008","unstructured":"Rohatgi S, Ganju P, Sehgal D . Systematic design and testing of nested (RT-) PCR primers for specific amplification of mouse rearranged\/expressed immunoglobulin variable region genes from small number of B cells. J Immunol Methods 2008; 339: 205\u2013219.","journal-title":"J Immunol Methods"},{"key":"BFcmi201033_CR26","doi-asserted-by":"publisher","first-page":"731","DOI":"10.5483\/BMBRep.2009.42.11.731","volume":"42","author":"KW Lee","year":"2009","unstructured":"Lee KW, Hur BU, Song SY, Choi HJ, Shin SH, Cha SH . Methods for rapid identification of a functional single-chain variable fragment using alkaline phosphatase fusion. BMB Rep 2009; 42: 731\u2013736.","journal-title":"BMB Rep"},{"key":"BFcmi201033_CR27","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/j.imbio.2008.10.007","volume":"214","author":"D Wang","year":"2009","unstructured":"Wang D, Lu TY, Liu HB . Characterization and genetic diversity of the sturgeon Acipenser schrenskii Ig heavy chain. Immunobiology 2009; 214: 359\u2013366.","journal-title":"Immunobiology"},{"key":"BFcmi201033_CR28","doi-asserted-by":"publisher","first-page":"7132","DOI":"10.4049\/jimmunol.178.11.7132","volume":"178","author":"LR Miranda","year":"2007","unstructured":"Miranda LR, Duval M, Doherty H, Seaman MS, Posner MR, Cavacini LA . The neutralization properties of a HIV-specific antibody are markedly altered by glycosylation events outside the antigen-binding domain. J Immunol 2007; 178: 7132\u20137138.","journal-title":"J Immunol"},{"key":"BFcmi201033_CR29","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1172\/JCI6837","volume":"104","author":"CS Goodyear","year":"1999","unstructured":"Goodyear CS, O'Hanlon GM, Plomp JJ, Wagner ER, Morrison I, Veitch J et al. Monoclonal antibodies raised against Guillain\u2013Barr\u00e9 syndrome-associated Campylobacter jejuni lipopolysaccharides react with neuronal gangliosides and paralyze muscle\u2013nerve preparations. J Clin Invest 1999; 104: 697\u2013708.","journal-title":"J Clin Invest"},{"key":"BFcmi201033_CR30","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/S1074-7613(00)80437-9","volume":"4","author":"W Fang","year":"1996","unstructured":"Fang W, Mueller DL, Pennell CA, Rivard JJ, Li YS, Hardy RR et al. Frequent aberrant immunoglobulin gene rearrangements in pro-B cells revealed by a bcl-xL transgene. Immunity 1996; 4: 291\u2013299.","journal-title":"Immunity"}],"container-title":["Cellular & Molecular Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/cmi201033.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/cmi201033","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/cmi201033.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T10:10:48Z","timestamp":1672740648000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/cmi201033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,26]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["BFcmi201033"],"URL":"https:\/\/doi.org\/10.1038\/cmi.2010.33","relation":{},"ISSN":["1672-7681","2042-0226"],"issn-type":[{"value":"1672-7681","type":"print"},{"value":"2042-0226","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,26]]},"assertion":[{"value":"31 March 2010","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 May 2010","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 May 2010","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 July 2010","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}