{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T20:15:41Z","timestamp":1778012141245,"version":"3.51.4"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Exp Mol Med"],"published-print":{"date-parts":[[2017,8]]},"DOI":"10.1038\/emm.2017.107","type":"journal-article","created":{"date-parts":[[2017,8,4]],"date-time":"2017-08-04T07:00:48Z","timestamp":1501830048000},"page":"e362-e362","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["dNP2-ctCTLA-4 inhibits German cockroach extract-induced allergic airway inflammation and hyper-responsiveness via inhibition of Th2 responses"],"prefix":"10.1038","volume":"49","author":[{"given":"Sangho","family":"Lim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung","family":"Ho Sohn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ja-Hyun","family":"Koo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung-Won","family":"Park","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Je-Min","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,8,4]]},"reference":[{"key":"BFemm2017107_CR1","doi-asserted-by":"publisher","first-page":"1720","DOI":"10.1164\/ajrccm.161.5.9903102","volume":"161","author":"J Bousquet","year":"2000","unstructured":"Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM . Asthma. From bronchoconstriction to airways inflammation and remodeling. Am J Respir Crit Care Med 2000; 161: 1720\u20131745.","journal-title":"Am J Respir Crit Care Med"},{"key":"BFemm2017107_CR2","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1016\/j.jaip.2015.06.019","volume":"3","author":"MA Calderon","year":"2015","unstructured":"Calderon MA, Kleine-Tebbe J, Linneberg A, De Blay F, Hernandez Fernandez de Rojas D, Virchow JC et al. House dust mite respiratory allergy: an overview of current therapeutic strategies. J Allergy Clin Immunol Pract 2015; 3: 843\u2013855.","journal-title":"J Allergy Clin Immunol Pract"},{"key":"BFemm2017107_CR3","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1111\/j.1365-2222.2005.02328.x","volume":"35","author":"G D'Amato","year":"2005","unstructured":"D'Amato G, Liccardi G, D'Amato M, Holgate S . Environmental risk factors and allergic bronchial asthma. Clin Exp Allergy 2005; 35: 1113\u20131124.","journal-title":"Clin Exp Allergy"},{"key":"BFemm2017107_CR4","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1067\/mai.2001.112854","volume":"107","author":"LK Arruda","year":"2001","unstructured":"Arruda LK, Vailes LD, Ferriani VP, Santos AB, Pomes A, Chapman MD . Cockroach allergens and asthma. J Allergy Clin Immunol 2001; 107: 419\u2013428.","journal-title":"J Allergy Clin Immunol"},{"key":"BFemm2017107_CR5","doi-asserted-by":"publisher","first-page":"283","DOI":"10.4168\/aair.2015.7.3.283","volume":"7","author":"KY Jeong","year":"2015","unstructured":"Jeong KY, Son M, Lee JH, Hong CS, Park JW . Allergenic characterization of a novel allergen, homologous to chymotrypsin, from german cockroach. Allergy Asthma Immunol Res 2015; 7: 283\u2013289.","journal-title":"Allergy Asthma Immunol Res"},{"key":"BFemm2017107_CR6","doi-asserted-by":"publisher","first-page":"679","DOI":"10.4049\/jimmunol.1200694","volume":"189","author":"C Oseroff","year":"2012","unstructured":"Oseroff C, Sidney J, Tripple V, Grey H, Wood R, Broide DH et al. Analysis of T cell responses to the major allergens from German cockroach: epitope specificity and relationship to IgE production. J Immunol 2012; 189: 679\u2013688.","journal-title":"J Immunol"},{"key":"BFemm2017107_CR7","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1038\/mi.2011.47","volume":"5","author":"H Nakano","year":"2012","unstructured":"Nakano H, Free ME, Whitehead GS, Maruoka S, Wilson RH, Nakano K et al. Pulmonary CD103(+) dendritic cells prime Th2 responses to inhaled allergens. Mucosal Immunol 2012; 5: 53\u201365.","journal-title":"Mucosal Immunol"},{"key":"BFemm2017107_CR8","first-page":"983149","volume":"2013","author":"L Xu","year":"2013","unstructured":"Xu L, Zhang M, Ma W, Jin S, Song W, He S . Cockroach allergen Bla g 7 promotes TIM4 expression in dendritic cells leading to Th2 polarization. Mediators Inflamm 2013; 2013: 983149.","journal-title":"Mediators Inflamm"},{"key":"BFemm2017107_CR9","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1164\/ajrccm\/146.2.374","volume":"146","author":"H Nakajima","year":"1992","unstructured":"Nakajima H, Iwamoto I, Tomoe S, Matsumura R, Tomioka H, Takatsu K et al. CD4+ T-lymphocytes and interleukin-5 mediate antigen-induced eosinophil infiltration into the mouse trachea. Am Rev Respir Dis 1992; 146: 374\u2013377.","journal-title":"Am Rev Respir Dis"},{"key":"BFemm2017107_CR10","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1146\/annurev.immunol.17.1.255","volume":"17","author":"M Wills-Karp","year":"1999","unstructured":"Wills-Karp M . Immunologic basis of antigen-induced airway hyperresponsiveness. Annu Rev Immunol 1999; 17: 255\u2013281.","journal-title":"Annu Rev Immunol"},{"key":"BFemm2017107_CR11","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1111\/all.12616","volume":"70","author":"O Boyman","year":"2015","unstructured":"Boyman O, Kaegi C, Akdis M, Bavbek S, Bossios A, Chatzipetrou A et al. EAACI IG Biologicals task force paper on the use of biologic agents in allergic disorders. Allergy 2015; 70: 727\u2013754.","journal-title":"Allergy"},{"key":"BFemm2017107_CR12","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1111\/j.1365-2222.2007.02650.x","volume":"37","author":"AA Cruz","year":"2007","unstructured":"Cruz AA, Lima F, Sarinho E, Ayre G, Martin C, Fox H et al. Safety of anti-immunoglobulin E therapy with omalizumab in allergic patients at risk of geohelminth infection. Clin Exp Allergy 2007; 37: 197\u2013207.","journal-title":"Clin Exp Allergy"},{"issue":"Suppl 5","key":"BFemm2017107_CR13","doi-asserted-by":"publisher","first-page":"S244","DOI":"10.1513\/AnnalsATS.201407-304AW","volume":"11","author":"DE Davies","year":"2014","unstructured":"Davies DE . Epithelial barrier function and immunity in asthma. Ann Am Thorac Soc 2014; 11 (Suppl 5): S244\u2013S251.","journal-title":"Ann Am Thorac Soc"},{"key":"BFemm2017107_CR14","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1084\/jem.182.2.459","volume":"182","author":"MF Krummel","year":"1995","unstructured":"Krummel MF, Allison JP . CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation. J Exp Med 1995; 182: 459\u2013465.","journal-title":"J Exp Med"},{"key":"BFemm2017107_CR15","doi-asserted-by":"publisher","first-page":"2263","DOI":"10.1126\/science.282.5397.2263","volume":"282","author":"KM Lee","year":"1998","unstructured":"Lee KM, Chuang E, Griffin M, Khattri R, Hong DK, Zhang W et al. Molecular basis of T cell inactivation by CTLA-4. Science 1998; 282: 2263\u20132266.","journal-title":"Science"},{"key":"BFemm2017107_CR16","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1084\/jem.174.3.561","volume":"174","author":"PS Linsley","year":"1991","unstructured":"Linsley PS, Brady W, Urnes M, Grosmaire LS, Damle NK, Ledbetter JA . CTLA-4 is a second receptor for the B cell activation antigen B7. J Exp Med 1991; 174: 561\u2013569.","journal-title":"J Exp Med"},{"key":"BFemm2017107_CR17","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/1074-7613(94)90071-X","volume":"1","author":"TL Walunas","year":"1994","unstructured":"Walunas TL, Lenschow DJ, Bakker CY, Linsley PS, Freeman GJ, Green JM et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1: 405\u2013413.","journal-title":"Immunity"},{"key":"BFemm2017107_CR18","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1111\/j.1365-2249.2011.04405.x","volume":"165","author":"L Ying","year":"2011","unstructured":"Ying L, Fu Z, Luo J, Zhou C, Chen Y, Wang L et al. Cytotoxic T lymphocyte antigen 4 immunoglobulin modified dendritic cells attenuate allergic airway inflammation and hyperresponsiveness by regulating the development of T helper type 1 (Th1)\/Th2 and Th2\/regulatory T cell subsets in a murine model of asthma. Clin Exp Immunol 2011; 165: 130\u2013139.","journal-title":"Clin Exp Immunol"},{"key":"BFemm2017107_CR19","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1165\/ajrcmb.17.3.2679","volume":"17","author":"AJ Van Oosterhout","year":"1997","unstructured":"Van Oosterhout AJ, Hofstra CL, Shields R, Chan B, Van Ark I, Jardieu PM et al. Murine CTLA4-IgG treatment inhibits airway eosinophilia and hyperresponsiveness and attenuates IgE upregulation in a murine model of allergic asthma. Am J Respir Cell Mol Biol 1997; 17: 386\u2013392.","journal-title":"Am J Respir Cell Mol Biol"},{"key":"BFemm2017107_CR20","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.jaip.2013.10.006","volume":"2","author":"R Abdelshaeed","year":"2014","unstructured":"Abdelshaeed R, Griffiths GB, Neighbour H . Use of abatacept in eosinophilic asthma. J Allergy Clin Immunol Pract 2014; 2: 220\u2013221.","journal-title":"J Allergy Clin Immunol Pract"},{"key":"BFemm2017107_CR21","doi-asserted-by":"publisher","first-page":"8244","DOI":"10.1038\/ncomms9244","volume":"6","author":"S Lim","year":"2015","unstructured":"Lim S, Kim WJ, Kim YH, Lee S, Koo JH, Lee JA et al. dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis. Nat Commun 2015; 6: 8244.","journal-title":"Nat Commun"},{"key":"BFemm2017107_CR22","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1159\/000347034","volume":"161","author":"KY Jeong","year":"2013","unstructured":"Jeong KY, Kim CR, Park J, Han IS, Park JW, Yong TS . Identification of novel allergenic components from German cockroach fecal extract by a proteomic approach. Int Arch Allergy Immunol 2013; 161: 315\u2013324.","journal-title":"Int Arch Allergy Immunol"},{"key":"BFemm2017107_CR23","doi-asserted-by":"publisher","first-page":"e0155689","DOI":"10.1371\/journal.pone.0155689","volume":"11","author":"S Lim","year":"2016","unstructured":"Lim S, Lee JA, Koo JH, Kang TG, Ha SJ, Choi JM . Cell type preference of a novel human derived cell-permeable peptide dNP2 and TAT in murine splenic immune cells. PLoS ONE 2016; 11: e0155689.","journal-title":"PLoS ONE"},{"key":"BFemm2017107_CR24","doi-asserted-by":"publisher","first-page":"46","DOI":"10.3389\/fphar.2013.00046","volume":"4","author":"SS Possa","year":"2013","unstructured":"Possa SS, Leick EA, Prado CM, Martins MA, Tiberio IF . Eosinophilic inflammation in allergic asthma. Front Pharmacol 2013; 4: 46.","journal-title":"Front Pharmacol"},{"issue":"2 Suppl","key":"BFemm2017107_CR25","doi-asserted-by":"publisher","first-page":"17S","DOI":"10.1378\/chest.111.2_Supplement.17S","volume":"111","author":"PJ Barnes","year":"1997","unstructured":"Barnes PJ . Current therapies for asthma. Promise and limitations. Chest 1997; 111 (2 Suppl): 17S\u201326S.","journal-title":"Chest"},{"key":"BFemm2017107_CR26","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1038\/nm.2754","volume":"18","author":"CA Akdis","year":"2012","unstructured":"Akdis CA . Therapies for allergic inflammation: refining strategies to induce tolerance. Nat Med 2012; 18: 736\u2013749.","journal-title":"Nat Med"},{"key":"BFemm2017107_CR27","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1007\/s11882-016-0650-5","volume":"16","author":"HT Tan","year":"2016","unstructured":"Tan HT, Sugita K, Akdis CA . Novel biologicals for the treatment of allergic diseases and asthma. Curr Allergy Asthma Rep 2016; 16: 70.","journal-title":"Curr Allergy Asthma Rep"},{"key":"BFemm2017107_CR28","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.pupt.2015.01.006","volume":"31","author":"M Caminati","year":"2015","unstructured":"Caminati M, Senna G, Guerriero M, Dama AR, Chieco-Bianchi F, Stefanizzi G et al. Omalizumab for severe allergic asthma in clinical trials and real-life studies: what we know and what we should address. Pulm Pharmacol Ther 2015; 31: 28\u201335.","journal-title":"Pulm Pharmacol Ther"},{"key":"BFemm2017107_CR29","doi-asserted-by":"publisher","first-page":"27","DOI":"10.2217\/imt.15.102","volume":"8","author":"V Fainardi","year":"2016","unstructured":"Fainardi V, Pisi G, Chetta A . Mepolizumab in the treatment of severe eosinophilic asthma. Immunotherapy 2016; 8: 27\u201334.","journal-title":"Immunotherapy"},{"key":"BFemm2017107_CR30","first-page":"w13581","volume":"142","author":"J von Kempis","year":"2012","unstructured":"von Kempis J, Dudler J, Hasler P, Kyburz D, Tyndall A, Zufferey P et al. Use of abatacept in rheumatoid arthritis. Swiss Med Wkly 2012; 142: w13581.","journal-title":"Swiss Med Wkly"},{"key":"BFemm2017107_CR31","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1164\/rccm.201207-1205OC","volume":"187","author":"AD Parulekar","year":"2013","unstructured":"Parulekar AD, Boomer JS, Patterson BM, Yin-Declue H, Deppong CM, Wilson BS et al. A randomized controlled trial to evaluate inhibition of T-cell costimulation in allergen-induced airway inflammation. Am J Respir Crit Care Med 2013; 187: 494\u2013501.","journal-title":"Am J Respir Crit Care Med"},{"key":"BFemm2017107_CR32","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1038\/nm1385","volume":"12","author":"JM Choi","year":"2006","unstructured":"Choi JM, Ahn MH, Chae WJ, Jung YG, Park JC, Song HM et al. Intranasal delivery of the cytoplasmic domain of CTLA-4 using a novel protein transduction domain prevents allergic inflammation. Nat Med 2006; 12: 574\u2013579.","journal-title":"Nat Med"},{"key":"BFemm2017107_CR33","doi-asserted-by":"publisher","first-page":"19875","DOI":"10.1073\/pnas.0805198105","volume":"105","author":"JM Choi","year":"2008","unstructured":"Choi JM, Kim SH, Shin JH, Gibson T, Yoon BS, Lee DH et al. Transduction of the cytoplasmic domain of CTLA-4 inhibits TcR-specific activation signals and prevents collagen-induced arthritis. Proc Natl Acad Sci USA 2008; 105: 19875\u201319880.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFemm2017107_CR34","doi-asserted-by":"publisher","first-page":"33","DOI":"10.4110\/in.2016.16.1.33","volume":"16","author":"S Lim","year":"2016","unstructured":"Lim S, Koo JH, Choi JM . Use of cell-penetrating peptides in dendritic cell-based vaccination. Immune Netw 2016; 16: 33\u201343.","journal-title":"Immune Netw"},{"key":"BFemm2017107_CR35","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1007\/s10059-012-0284-y","volume":"34","author":"S Lim","year":"2012","unstructured":"Lim S, Kim WJ, Kim YH, Choi JM . Identification of a novel cell-penetrating peptide from human phosphatidate phosphatase LPIN3. Mol Cells 2012; 34: 577\u2013582.","journal-title":"Mol Cells"},{"key":"BFemm2017107_CR36","doi-asserted-by":"publisher","first-page":"8117","DOI":"10.1007\/s11033-014-3711-7","volume":"41","author":"JH Koo","year":"2014","unstructured":"Koo JH, Yoon H, Kim WJ, Lim S, Park HJ, Choi JM . Cell membrane penetrating function of the nuclear localization sequence in human cytokine IL-1alpha. Mol Biol Rep 2014; 41: 8117\u20138126.","journal-title":"Mol Biol Rep"},{"key":"BFemm2017107_CR37","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1182\/blood.V91.7.2240","volume":"91","author":"RT Palframan","year":"1998","unstructured":"Palframan RT, Collins PD, Williams TJ, Rankin SM . Eotaxin induces a rapid release of eosinophils and their progenitors from the bone marrow. Blood 1998; 91: 2240\u20132248.","journal-title":"Blood"},{"key":"BFemm2017107_CR38","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1165\/ajrcmb\/8.4.349","volume":"8","author":"MB Resnick","year":"1993","unstructured":"Resnick MB, Weller PF . Mechanisms of eosinophil recruitment. Am J Respir Cell Mol Biol 1993; 8: 349\u2013355.","journal-title":"Am J Respir Cell Mol Biol"},{"key":"BFemm2017107_CR39","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1016\/S1081-1206(10)63303-0","volume":"86","author":"Y Obase","year":"2001","unstructured":"Obase Y, Shimoda T, Mitsuta K, Matsuo N, Matsuse H, Kohno S . Correlation between airway hyperresponsiveness and airway inflammation in a young adult population: eosinophil, ECP, and cytokine levels in induced sputum. Ann Allergy Asthma Immunol 2001; 86: 304\u2013310.","journal-title":"Ann Allergy Asthma Immunol"},{"key":"BFemm2017107_CR40","doi-asserted-by":"publisher","first-page":"2254","DOI":"10.1172\/JCI119763","volume":"100","author":"CM Evans","year":"1997","unstructured":"Evans CM, Fryer AD, Jacoby DB, Gleich GJ, Costello RW . Pretreatment with antibody to eosinophil major basic protein prevents hyperresponsiveness by protecting neuronal M2 muscarinic receptors in antigen-challenged guinea pigs. J Clin Invest 1997; 100: 2254\u20132262.","journal-title":"J Clin Invest"},{"key":"BFemm2017107_CR41","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/S0192-0561(97)00085-4","volume":"19","author":"AD Kraneveld","year":"1997","unstructured":"Kraneveld AD, Folkerts G, Van Oosterhout AJ, Nijkamp FP . Airway hyperresponsiveness: first eosinophils and then neuropeptides. Int J Immunopharmacol 1997; 19: 517\u2013527.","journal-title":"Int J Immunopharmacol"},{"key":"BFemm2017107_CR42","doi-asserted-by":"publisher","first-page":"253","DOI":"10.2174\/187152806779010909","volume":"5","author":"RK Kumar","year":"2006","unstructured":"Kumar RK, Webb DC, Herbert C, Foster PS . Interferon-gamma as a possible target in chronic asthma. Inflamm Allergy Drug Targets 2006; 5: 253\u2013256.","journal-title":"Inflamm Allergy Drug Targets"}],"container-title":["Experimental &amp; Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/emm2017107.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/emm2017107","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/doifinder\/10.1038\/emm.2017.107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/emm2017107.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T17:20:50Z","timestamp":1684430450000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/emm2017107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["BFemm2017107"],"URL":"https:\/\/doi.org\/10.1038\/emm.2017.107","relation":{},"ISSN":["1226-3613","2092-6413"],"issn-type":[{"value":"1226-3613","type":"print"},{"value":"2092-6413","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8]]},"assertion":[{"value":"14 October 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2017","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 March 2017","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 August 2017","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"J-MC and SL are inventors who have patent applications describing the use of dNP2 and therapeutic use of dNP2-ctCTLA-4 in asthma. The remaining authors declare no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}