{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T09:14:18Z","timestamp":1696065258298},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1976,2,1]],"date-time":"1976-02-01T00:00:00Z","timestamp":191980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1976,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Exogenously added N<jats:sup>6<\/jats:sup>, O<jats:sup>2\u2032<\/jats:sup>\u2010dibutyryladenosine\u20103\u2032\u20105\u2032\u2010cyclic phosphate (DBcAMP) exerted a biphasic effect on the <jats:italic>in vitro<\/jats:italic> primary response of mouse spleen cells to sheep erythrocytes (SRBC). High concentrations of DBcAMP were markedly suppressive, whereas lower concentrations brought about significant increases in the peak numbers of antibody\u2010forming cells. The kinetics of the response were not altered by DBcAMP. A direct effect of DBcAMP on bone\u2010marrow derived lymphocytes was suggested because this nucleotide increased the <jats:italic>in vitro<\/jats:italic> response of \u201cnude\u201d mouse spleen cells to SRBC. The magnitude of the response, however, was considerably less than that mounted by spleen cells of normal mice. These results show that DBcAMP can have either enhancing or suppressive effects on antibody formation and these effects are dependent upon nucleotide concentration, antigen\u2010dosage and time of assay. The effects of this cyclic nucleotide differed in two major respects from those of the synthetic polyribonucleotide adjuvant, polyadenylic acid\u2010polyuridylic acid (poly A:U) complexes. Thus poly (A:U) decreased the time of peak response <jats:italic>in vitro<\/jats:italic> but did not increase the magnitude of the response.<\/jats:p>","DOI":"10.1038\/icb.1976.1","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T09:50:45Z","timestamp":1196157045000},"page":"1-10","source":"Crossref","is-referenced-by-count":3,"title":["EFFECTS OF DIBUTYRYLCYCLIC AMP ON THE <i>IN VITRO<\/i> PRIMARY RESPONSE OF MOUSE SPLEEN CELLS TO SHEEP ERYTHROCYTES"],"prefix":"10.1111","volume":"54","author":[{"given":"John J","family":"Marchalonis","sequence":"first","affiliation":[{"name":"Molecular Immunology Laboratory, The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital Victoria Australia 3050"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Smith","sequence":"additional","affiliation":[{"name":"Molecular Immunology Laboratory, The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital Victoria Australia 3050"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1976,2]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1976.1","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb19761","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb19761.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1976.1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb19761.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T04:26:45Z","timestamp":1695961605000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1976.1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1976,2]]}},"alternative-id":["10.1038\/icb.1976.1"],"URL":"https:\/\/doi.org\/10.1038\/icb.1976.1","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1976,2]]}}}