{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:32:59Z","timestamp":1759332779415},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1976,8,1]],"date-time":"1976-08-01T00:00:00Z","timestamp":207705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1976,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Target cells (P\u2010815 mastocytoma cells) infected with ectromelia virus became susceptible to lysis by H\u20102 compatible specific effector T cells within one hour of exposure of the cells to virus. This is long before viral progeny are produced and shed from the cell.<\/jats:p><jats:p>Polyacrylamide gel electrophoresis (PAGE) profiles of the plasma membranes from infected and uninfected P\u2010815 cells pulsed with <jats:sup>35<\/jats:sup>S\u2010methionine for one or a few hours after infection with virus were very complex and showed no consistent differences. P\u2010815 cells, infected with ectromelia virus in the presence of an inhibitor of protein synthesis, pactamycin. slowly became susceptible to cell mediated lysis when the pactamycin was removed. The number of polypeptide species synthesized under these conditions was reduced to only three, of molecular weights between 10,000\u201350,000 daltons.<\/jats:p><jats:p>Specific, newly synthesized membrane components recognized by mouse convalescent sera were isolated by immune complexion and examined by PAGE. Six polypeptide bands were seen, the major one correlating with one observed in the pactamycin experiment. The results suggested that the convalescent serum recognized both viral and host cell coded antigens. The significance of these findings is discussed.<\/jats:p>","DOI":"10.1038\/icb.1976.35","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T09:52:32Z","timestamp":1196157152000},"page":"349-363","source":"Crossref","is-referenced-by-count":27,"title":["CYTOTOXIC T CELLS RECOGNIZE VERY EARLY, MINOR CHANGES IN ECTROMELIA VIRUS\u2010INFECTED TARGET CELLS"],"prefix":"10.1111","volume":"54","author":[{"given":"DC","family":"Jackson","sequence":"first","affiliation":[{"name":"Department of Microbiology, John Curtin School of Medical Research, Australian National University Canberra A.C.T., Australia 2600"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GL","family":"Ada","sequence":"additional","affiliation":[{"name":"Department of Microbiology, John Curtin School of Medical Research, Australian National University Canberra A.C.T., Australia 2600"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R Tha","family":"Hla","sequence":"additional","affiliation":[{"name":"Department of Microbiology, John Curtin School of Medical Research, Australian National University Canberra A.C.T., Australia 2600"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1976,8]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1976.35","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb197635","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb197635.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1976.35","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb197635.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T03:51:18Z","timestamp":1696045878000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1976.35"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,8]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1976,8]]}},"alternative-id":["10.1038\/icb.1976.35"],"URL":"https:\/\/doi.org\/10.1038\/icb.1976.35","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1976,8]]}}}