{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T21:08:42Z","timestamp":1699477722763},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[1976,12,1]],"date-time":"1976-12-01T00:00:00Z","timestamp":218246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1976,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The role of adherent cells in an <jats:italic>in vitro<\/jats:italic> secondary response to ectromelia virus infection was investigated. Spleen cells from ectromelia\u2010primed mice (\u201cresponder\u201d cells) depleted of adherent cells by either carbonyl iron treatment, adherence to plastic or passage through cotton wool columns had a markedly decreased capacity to produce a secondary response, as indicated by decreased T cell\u2010mediated cytotoxicity against virus\u2010infected target cells, when cultured with virus\u2010infected \u201cstimulator\u201d cells. The secondary response was restored by the addition of peritoneal cells from cither normal or ectromelia\u2010immune mice. Small numbers of peritoneal cells completely reconstituted the response within a certain dose range but larger numbers produced a marked inhibition of the response. Spleen cells were less effective in restoring the response. The peritoneal cells were not merely acting as additional, infected \u201cstimulator\u201d or antigen\u2010presenting cells, since they could be added as late as 3 days after culture. Reconstituting activity was not affected by pretreatment with anti\u2010<jats:italic>\u03b8<\/jats:italic> serum and complement and cell separation studies showed that the activity was associated mainly with Ig\u2010negative cells and that the active cell probably bears Ia antigens on its surface. These results indicate that the adherent cells involved are probably macrophages and that they act non\u2010specifically to produce optimum conditions for the specific response of T cells.<\/jats:p>","DOI":"10.1038\/icb.1976.57","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T09:53:49Z","timestamp":1196157229000},"page":"559-571","source":"Crossref","is-referenced-by-count":4,"title":["THE ROLE OF ADHERENT CELLS IN THE SECONDARY CELL\u2010MEDIATED RESPONSE <i>IN VITRO<\/i> TO A NATURAL POXVIRUS PATHOGEN"],"prefix":"10.1111","volume":"54","author":[{"given":"T","family":"Pang","sequence":"first","affiliation":[{"name":"Department of Microbiology, John Curtin School of Medical Research, Australian National University Canberra Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RV","family":"Blanden","sequence":"additional","affiliation":[{"name":"Department of Microbiology, John Curtin School of Medical Research, Australian National University Canberra Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1976,12]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1976.57","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb197657","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb197657.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1976.57","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb197657.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T10:51:11Z","timestamp":1695984671000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1976.57"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1976,12]]}},"alternative-id":["10.1038\/icb.1976.57"],"URL":"https:\/\/doi.org\/10.1038\/icb.1976.57","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1976,12]]}}}