{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T12:23:51Z","timestamp":1699100631504},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1977,8,1]],"date-time":"1977-08-01T00:00:00Z","timestamp":239241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1977,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The origin of the fasciculation of skeletal muscle produced by funnel\u2010web spider venom (FSV) has been examined in mouse phrenic nerve hemi\u2010diaphragm preparations. FSV from male spiders at concentrations greater than 10<jats:sup>\u22126<\/jats:sup> g\/ml invariably produced muscle fasciculation which could be prevented by d\u2010tubocurarine (14\u03bcM), tetrodotoxin (0\u00b73 \u03bcM) or by increasing the external magnesium concentration or calcium concentration. Diphenylhydantoin (3\u00b76 \u00d7 10<jats:sup>\u22125<\/jats:sup> M) was able to reduce these fasciculations in some experiments. In curarized preparations, multiple end plate potentials (EPPs) in response to single stimuli and bursts of spontaneous EPPs were seen in the presence of FSV (10<jats:sup>\u22125<\/jats:sup> g\/ml). Extracellular recordings from phrenic nerves in the presence of FSV (10<jats:sup>\u22121<\/jats:sup> g\/ml) revealed additional components in compound action potentials elicited by single stimuli, and \u201cspontaneous\u201d electrical activity was observed in unstimulated nerves. This spontaneous activity was abolished by raising the divalent cation concentration in the bathing solution. These results suggest that a primary site of action of FSV is the surface membrane of nerve fibres and that muscle faseiculation arises as a consequence of spontaneous action potentials produced by the venom in motor nerves.<\/jats:p>","DOI":"10.1038\/icb.1977.44","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T09:56:34Z","timestamp":1196157394000},"page":"453-461","source":"Crossref","is-referenced-by-count":15,"title":["THE ORIGIN OF THE MUSCLE FASCICULATION CAUSED BY FUNNEL\u2010WEB SPIDER VENOM"],"prefix":"10.1111","volume":"55","author":[{"given":"Peter W","family":"Gage","sequence":"first","affiliation":[{"name":"School of Physiology and Pharmacology, University of New South Wales Kensington, 2033 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Spence","sequence":"additional","affiliation":[{"name":"School of Physiology and Pharmacology, University of New South Wales Kensington, 2033 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1977,8]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1977.44","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb197744","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb197744.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1977.44","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb197744.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T08:07:03Z","timestamp":1695974823000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1977.44"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,8]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1977,8]]}},"alternative-id":["10.1038\/icb.1977.44"],"URL":"https:\/\/doi.org\/10.1038\/icb.1977.44","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1977,8]]}}}