{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:21:10Z","timestamp":1758709270367},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1977,10,1]],"date-time":"1977-10-01T00:00:00Z","timestamp":244512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[1977,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>When virus\u2010immune cytotoxic T cells were generated in secondary responses <jats:italic>in vitro<\/jats:italic> to syngeneic, infected stimulator cells, they only killed infected targets that shared <jats:italic>H\u20102K<\/jats:italic> or <jats:italic>H\u20102D<\/jats:italic> genes with the T cells and stimulator cells. <jats:italic>H\u20102I<\/jats:italic> region compatibility was neither sufficient nor necessary. The role of <jats:italic>H\u20102K, H\u20102D<\/jats:italic> and <jats:italic>H\u20102I<\/jats:italic> genes in the interaction between memory T cells and infected stimulator cells was then investigated. Initial attempts failed because of escape of virus into the responder population. Steps were taken to eliminate this problem; confirming their efficacy was the finding that when (CBA\/H \u00d7 BALB\/c) F<jats:sub>1<\/jats:sub> memory T cells were stimulated by either parental type, the cytotoxic T cells generated only killed the target cell type syngeneic to the stimulator cell used to induce the response. Using recombinant strain macrophages as infected stimulator cells, it was further shown that K or <jats:italic>D<\/jats:italic> region sharing between stimulator and responder cells was sufficient to induce the response. <jats:italic>I<\/jats:italic> region homology, on the other hand, although stimulating high levels of <jats:sup>3<\/jats:sup>H\u2010thymidine incorporation, did not give rise to significant amounts of cytotoxic activity against virus\u2010infected target cells. These results are compatible with the interpretation that the inductive signal to cytotoxic T cell precursors (memory T cells) in the <jats:italic>in vitro<\/jats:italic> secondary response is an antigenic pattern containing virus\u2010induced and self <jats:italic>H\u20102K<\/jats:italic> or <jats:italic>H\u20102D<\/jats:italic> components; the response of the cytotoxic cells may be facilitated by a \u201chelper\u201d, DNA\u2010synthesizing subclass which responds to antigenic patterns containing both virus\u2010induced and <jats:italic>I<\/jats:italic> region\u2010determined components.<\/jats:p><jats:p>Evidence from cytotoxic T cell induction with semi\u2010compatible infected macrophages shows that <jats:italic>H\u20102K<\/jats:italic> (or <jats:italic>H\u20102D<\/jats:italic>) antigens and viral antigen must be in the same cell membrane for efficient stimulation to occur. This suggests that both the antigens, and the T cell receptors for them, are closely associated in the infected cell and T cell membranes respectively.<\/jats:p>","DOI":"10.1038\/icb.1977.54","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T09:57:07Z","timestamp":1196157427000},"page":"549-559","source":"Crossref","is-referenced-by-count":9,"title":["GENETIC FACTORS IN THE STIMULATION OF T CELL RESPONSES AGAINST ECTROMELIA VIRUS\u2010INFECTED CELLS: ROLE OF <i>H\u20102K, H\u20102D<\/i> AND <i>H\u20102I<\/i> GENES"],"prefix":"10.1111","volume":"55","author":[{"given":"T","family":"Pang","sequence":"first","affiliation":[{"name":"Department of Microbiology, John Curtin School of Medical Research, Australian National University Canberra Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RV","family":"Blanden","sequence":"additional","affiliation":[{"name":"Department of Microbiology, John Curtin School of Medical Research, Australian National University Canberra Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1977,10]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1977.54","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb197754","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb197754.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1977.54","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb197754.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T02:34:50Z","timestamp":1696041290000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1977.54"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,10]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1977,10]]}},"alternative-id":["10.1038\/icb.1977.54"],"URL":"https:\/\/doi.org\/10.1038\/icb.1977.54","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1977,10]]}}}