{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T02:19:21Z","timestamp":1744165161800},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1980,8,1]],"date-time":"1980-08-01T00:00:00Z","timestamp":333936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1980,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Attempts have been made to produce discriminatory immunodiagnostic reagents for infections of sheep with the larval stages of <jats:italic>Taenia hydatigena. Taenia ovis and Echinoccus granulosus<\/jats:italic>. Using cells from mice hyperimmunized with <jats:italic>T. hydatigena<\/jats:italic> antigenic preparations for fusion, a hybridoma antibody which bound to <jats:italic>T hydatigena<\/jats:italic> larval antigens, but not to <jats:italic>T. ovis<\/jats:italic> or <jats:italic>E. granulosus<\/jats:italic> larval antigens, was selected. A competitive radioimmunoassay using the labelled hybridoma antibody and a crude parasite antigen mixture was devised as an immunodiagnostic test for experimental <jats:italic>T hydatigena<\/jats:italic> infection. Sera from sheep exposed to 1000 or more <jats:italic>T. hydatigena<\/jats:italic> eggs were unequivocally positive but sera from 2 sheep with a monospecific infection with <jats:italic>T. ovis<\/jats:italic> were also positive. Sera from <jats:italic>E. granulosus<\/jats:italic>\u2010infected sheep were negative and, although the numbers of sera involved were small, such discrimination has not been achieved by previous serologic methods. The discussion emphasizes various difficulties which have been encountered in the development of highly sensitive as well as specific hybridoma\u2010based immunodiagnostic reagents for the detection of larval cestode infections in sheep.<\/jats:p>","DOI":"10.1038\/icb.1980.33","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T10:09:12Z","timestamp":1196158152000},"page":"339-350","source":"Crossref","is-referenced-by-count":13,"title":["HYBRIDOMA ANTIBODY IMMUNOASSAYS FOR THE DETECTION OF PARASITIC INFECTION: ATTEMPTS TO PRODUCE AN IMMUNODIAGNOSTIC REAGENT FOR A LARVAL TAENIID CESTODE INFECTION"],"prefix":"10.1111","volume":"58","author":[{"given":"Philip S","family":"Craig","sequence":"first","affiliation":[{"name":"Department of Immunology, John Curtin School of Medical Research, Australian National University Canberra A.C.T. 2600"},{"name":"Laboratory of Immunoparasitology, The Walter and Eliza Hall Institute of Medical Research Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham F","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Immunology, John Curtin School of Medical Research, Australian National University Canberra A.C.T. 2600"},{"name":"Laboratory of Immunoparasitology, The Walter and Eliza Hall Institute of Medical Research Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathy M","family":"Cruise","sequence":"additional","affiliation":[{"name":"Department of Immunology, John Curtin School of Medical Research, Australian National University Canberra A.C.T. 2600"},{"name":"Laboratory of Immunoparasitology, The Walter and Eliza Hall Institute of Medical Research Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael D","family":"Rickard","sequence":"additional","affiliation":[{"name":"Department of Immunology, John Curtin School of Medical Research, Australian National University Canberra A.C.T. 2600"},{"name":"Laboratory of Immunoparasitology, The Walter and Eliza Hall Institute of Medical Research Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1980,8]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1980.33","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198033","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198033.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1980.33","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb198033.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T03:19:58Z","timestamp":1695957598000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1980.33"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,8]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1980,8]]}},"alternative-id":["10.1038\/icb.1980.33"],"URL":"https:\/\/doi.org\/10.1038\/icb.1980.33","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1980,8]]}}}