{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:16:53Z","timestamp":1758637013148},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1981,4,1]],"date-time":"1981-04-01T00:00:00Z","timestamp":354931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1981,4]]},"abstract":"Summary<\/jats:title>Lymphocytes from sheep blood and lymph were analysed for the percentages of cells which had receptors for Fc\u03bc, Fc\u03b3 or C\u2032, which had surface immunoglobulin (sIg) or which could form rosettes with sheep red blood cells in the presence of saline (E) or 14% Ficoll\u2010saline (Efic<\/jats:sub>). Compared with lymphocytes prepared by treating blood with carbonyl iron followed by glycerol lysis of the red cells, lymphocytes prepared by Ficoll\u2010Paque flotation were enriched for B\u2010cells (sIg+<\/jats:sup>), depleted of T\u2010cells (Efic<\/jats:sub>+<\/jats:sup>) and retained a high proportion of phagocytic cells. Efferent popliteal lymph contained almost twice the percentage of Efic<\/jats:sub>+<\/jats:sup> cells as did blood prepared by glycerol lysis, but only half the percentage of sIg+<\/jats:sup> cells. An unmarked or \u2018null\u2019 cell population was defined by subtracting the percentage of E fic+<\/jats:sup> and sIg+<\/jats:sup> cells from 100%. In glycerol lysed blood 39\u00b70% of the cells were \u2018null\u2019 compared with 18\u00b76% in lymph.<\/jats:p>Fractionation of lymphocytes on Percoll discontinuous density gradients allowed some separation of T, B and \u2018null\u2019 cells from each other. With blood lymphocytes, \u2018null\u2019 cells were concentrated in the least dense region (1\u00b701 g\/ml), B\u2010cells in the region of intermediate density (1\u00b705\u20101\u00b707 g\/ml) and T\u2010cells in the most dense regions (1\u00b708\u20101\u00b709 g\/ml). Separation of blood and lymph cells on nylon wool columns and anti\u2010immunoglobulin affinity plates showed that \u2018null\u2019 cells were relatively non\u2010adherent and that there was a small population of C receptor\u2010bearing lymphocytes which were not sIg +<\/jats:sup>.<\/jats:p>Phytohaemagglutin (PHA) stimulation of lymphocytes prepared from blood, using Ficoll\u2010Paque or glycerol lysis and from lymph, showed that all three preparations had similar dose\u2010response curves irrespective of their T:B cell ratios. Interestingly, the \u2018null\u2019 cell enriched fraction from Percoll gradients also responded well to PHA.<\/jats:p>It was concluded that sheep blood contains three major populations, T, B and \u2018null\u2019 and that T\u2010cells (Efic<\/jats:sub>+<\/jats:sup>) are relatively concentrated in efferent popliteal lymph compared to blood. Some separation of these three cell types could be obtained using Percoll density gradients, nylon wool columns or anti\u2010immunoglobulin affinity plates. Comparing the PHA responsiveness of lymphocytes from different layers of the Percoll gradients, it was concluded that the sheep blood \u2018null\u2019 cells should be considered to be part of the T\u2010cell population.<\/jats:p>","DOI":"10.1038\/icb.1981.10","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T10:12:36Z","timestamp":1196158356000},"page":"143-155","source":"Crossref","is-referenced-by-count":15,"title":["LYMPHOCYTE SURFACE MARKERS IN SHEEP BLOOD AND LYMPH"],"prefix":"10.1111","volume":"59","author":[{"given":"PM","family":"Outteridge","sequence":"first","affiliation":[{"name":"C.S.I.R.O. Division of Animal Health, Animal Health Research Laboratory Private Bag No. 1, P.O. Parkville Victoria 3052"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KJ","family":"Fahey","sequence":"additional","affiliation":[{"name":"C.S.I.R.O. Division of Animal Health, Animal Health Research Laboratory Private Bag No. 1, P.O. Parkville Victoria 3052"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CS","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Veterinary Science Parkville Victoria 3052"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1981,4]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1981.10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198110","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198110.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1981.10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb198110.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T21:54:57Z","timestamp":1695938097000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1981.10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,4]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1981,4]]}},"alternative-id":["10.1038\/icb.1981.10"],"URL":"https:\/\/doi.org\/10.1038\/icb.1981.10","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1981,4]]}}}