{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T09:49:45Z","timestamp":1696067385342},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[1981,12,1]],"date-time":"1981-12-01T00:00:00Z","timestamp":376012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1981,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Agonist\u2010stimulated hydrolysis of phosphatidylinositol (PI) in cell membranes has been proposed to lead to an increase of cytosol calcium concentration and activation of the cellular response in certain smooth muscles and glands.<\/jats:p><jats:p>A method is described which allows the rapid, reproducible measurement of hydrolysis of phosphatidylinositol in mouse pancreas. The technique involves the <jats:italic>in vivo<\/jats:italic> labelling of the pancreas with <jats:italic>myo<\/jats:italic>\u2010[2\u2010<jats:sup>3<\/jats:sup>H] inositol. The majority of the label incorporated into phospholipids is in the form of PI. with only a small proportion of label in di\u2010 and tri phosphoinositides. Tissue pieces of the labelled pancreata are incubated <jats:italic>in vitro<\/jats:italic> in the presence or absence of secretagogues, and the PI in homogenates of these pieces is precipitated with trichloroacetic acid. No PI remains in the acid\u2010soluble supernatant. This technique does not require the time\u2010consuming extraction and chromatographic separation of lipids which has been necessary in other assays of PI hydrolysis. Using this method, we have confirmed that PI breakdown is stimulated by carbachol and cholecystokinin\u2010octapeptide in the presence or absence of extracellular Ca<jats:sup>2+<\/jats:sup>. Agonist\u2010Stimulated hydrolysis of PI was potentiated by extracellular Ca<jats:sup>2+<\/jats:sup>, and was not dependent on agonist\u2010activated Na<jats:sup>+<\/jats:sup> influx.<\/jats:p><jats:p>This technique will facilitate the investigation of the importance of PI breakdown in stimulus\u2010response coupling.<\/jats:p>","DOI":"10.1038\/icb.1981.68","type":"journal-article","created":{"date-parts":[[2007,11,27]],"date-time":"2007-11-27T10:15:08Z","timestamp":1196158508000},"page":"791-801","source":"Crossref","is-referenced-by-count":4,"title":["AGONIST\u2010STIMULATED BREAKDOWN OF <i>MYO<\/i>\u2010[2\u2010<sup>3<\/sup>H] INOSITOL\u2010LABELLED PHOSPHATIDYLINOSITOL IN MOUSE PANCREAS"],"prefix":"10.1111","volume":"59","author":[{"given":"Karin A","family":"Tennes","sequence":"first","affiliation":[{"name":"Department of Physiology, The University of Adelaide G.P.O, Box 498 Adelaide South Australia Australia 5000"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L","family":"Roberts","sequence":"additional","affiliation":[{"name":"Department of Physiology, The University of Adelaide G.P.O, Box 498 Adelaide South Australia Australia 5000"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1981,12]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1981.68","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198168","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198168.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1981.68","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb198168.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T11:13:57Z","timestamp":1695986037000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1981.68"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1981,12]]}},"alternative-id":["10.1038\/icb.1981.68"],"URL":"https:\/\/doi.org\/10.1038\/icb.1981.68","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1981,12]]}}}