{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T05:27:01Z","timestamp":1696051621104},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1982,10,1]],"date-time":"1982-10-01T00:00:00Z","timestamp":402278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1982,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Rabbit antisera have been produced to an acute myelomonocytic leukaemia (AMML)\u2010derived cell line (RC2a) and a histiocytic lymphoma derived cell line (U937) having macrophage characteristics. The antisera were screened by complement\u2010mediated cytotoxicity and immunofluorescence (cytofluorograph analysis) against separated leukaemic (122 patients plus 13 cell lines) and normal haematologic cell populations (60 preparations from 20 donors plus 10 B\u2010lymphoblastoid cell lines). The sera were absorbed with pooled B\u2010lymphoblastoid cell lines including the autologous B\u2010lymphoblastoid cell line to RC2a (CESS\u2010B) or alternatively with B\u2010CLL and T\u2010CLL cells. All leukaemic cell populations were confirmed using the markers SIg, E\u2010rosette receptor, cALL antigen, \u03b1\u2010naphthyl butyrate esterase and mydoperoxidase.<\/jats:p><jats:p>Rabbit anti\u2010RC2a (Adherent cells) (RARC2a(Ad)) and rabbit anti\u2010U937 (RAU937) recognised antigens common to immature myeloid monocyte and T\u2010lymphocyte lineage but did not react by cytotoxicity, absorption or cytofluorographic analysis with cells of B\u2010lymphocyte lineage (B\u2010lymphoblastoid or B\u2010CLL) and reacted only occasionally with cALL patients' cells (includes pre B phenotype). These sera reacted with peripheral blood monocytes but not with other mature blood leucocytes. RAU937 reacted with a major mononuclear population from normal marrow and with more differentiated myeloid leukaemia cells. RARC2a(Ad) and RAU937 detected overlapping subgroups of myeloid leukaemia (AMoL, AMML, AML and CML) patients and Null\u2010ALL and T\u2010ALL patients. These subgroups are now being examined for prognostic significance.<\/jats:p>","DOI":"10.1038\/icb.1982.53","type":"journal-article","created":{"date-parts":[[2007,8,16]],"date-time":"2007-08-16T15:30:52Z","timestamp":1187278252000},"page":"479-492","source":"Crossref","is-referenced-by-count":2,"title":["RABBIT ANTISERA TO CELL LINES RC2a AND U937: ANTIGENS EXPRESSED ON HUMAN LEUKAEMIC CELLS OF MYELOID, MONOCYTE AND T\u2010LYMPHOCYTE LINEAGE"],"prefix":"10.1111","volume":"60","author":[{"given":"Glenn","family":"Pilkington","sequence":"first","affiliation":[{"name":"Clinical Immunology and Immunogenetics Unit, Cancer Institute 481 Little Londsdale Street Melbourne Victoria, 3000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Clinical Immunology and Immunogenetics Unit, Cancer Institute 481 Little Londsdale Street Melbourne Victoria, 3000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Mackenzie","sequence":"additional","affiliation":[{"name":"Clinical Immunology and Immunogenetics Unit, Cancer Institute 481 Little Londsdale Street Melbourne Victoria, 3000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ray","family":"Bradley","sequence":"additional","affiliation":[{"name":"Biological Research Unit, Cancer Institute 481 Little Londsdale Street Melbourne Victoria, 3000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Hunter","sequence":"additional","affiliation":[{"name":"Clinical Immunology and Immunogenetics Unit, Cancer Institute 481 Little Londsdale Street Melbourne Victoria, 3000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Jose","sequence":"additional","affiliation":[{"name":"Clinical Immunology and Immunogenetics Unit, Cancer Institute 481 Little Londsdale Street Melbourne Victoria, 3000 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1982,10]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1982.53","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198253","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198253.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1982.53","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb198253.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T10:59:46Z","timestamp":1695985186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1982.53"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,10]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1982,10]]}},"alternative-id":["10.1038\/icb.1982.53"],"URL":"https:\/\/doi.org\/10.1038\/icb.1982.53","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1982,10]]}}}