{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T08:17:10Z","timestamp":1695975430121},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1983,4,1]],"date-time":"1983-04-01T00:00:00Z","timestamp":418003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1983,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>All six enzymes of <jats:italic>de novo<\/jats:italic> pyrimidine biosynthesis leading to the formation of UMP have been demonstrated in whole homogenates from two bovine <jats:italic>Babesia<\/jats:italic> species, <jats:italic>B. bovis<\/jats:italic> and <jats:italic>B. bigemina.<\/jats:italic> The specific activities of the respective enzymes were of the same order of magnitude as observed for the related parasite, <jats:italic>Plasmodium berghei.<\/jats:italic> The results indicate that both these parasites have the potential of obtaining their pyrimidine requirements by <jats:italic>de novo<\/jats:italic> synthesis.<\/jats:p><jats:p>Subcellular fractionation established that dihydroorotate dehydrogenase, the fourth enzyme of the pathway, was of a particulate nature. Mammalian respiratory chain inhibitors and ubiquinone analogues caused inhibition of the <jats:italic>Babesia<\/jats:italic> dihydroorotate dehydrogenase. As observed for other eukaryotic systems, the dehydrogenase appears to be linked to a respiratory chain via ubiquinone.<\/jats:p>","DOI":"10.1038\/icb.1983.22","type":"journal-article","created":{"date-parts":[[2007,8,13]],"date-time":"2007-08-13T13:07:14Z","timestamp":1187010434000},"page":"239-243","source":"Crossref","is-referenced-by-count":13,"title":["THE ENZYMES OF PYRIMIDINE BIOSYNTHESIS IN <i>BABESIA BOVIS<\/i> AND <i>BABESIA BIGEMINA<\/i>"],"prefix":"10.1111","volume":"61","author":[{"given":"Annette M","family":"Gero","sequence":"first","affiliation":[{"name":"School of Biochemistry, University of N.S.W. Kensington N.S.W. 2033 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J","family":"O'Sullivan","sequence":"additional","affiliation":[{"name":"School of Biochemistry, University of N.S.W. Kensington N.S.W. 2033 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian G","family":"Wright","sequence":"additional","affiliation":[{"name":"School of Biochemistry, University of N.S.W. Kensington N.S.W. 2033 Australia"},{"name":"CSIRO Division of Tropical Animal Science Indooroopilly Queensland 4068 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David F","family":"Mahoney","sequence":"additional","affiliation":[{"name":"CSIRO Division of Tropical Animal Science Indooroopilly Queensland 4068 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1983,4]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1983.22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198322","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198322.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1983.22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb198322.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T19:05:02Z","timestamp":1695927902000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1983.22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,4]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1983,4]]}},"alternative-id":["10.1038\/icb.1983.22"],"URL":"https:\/\/doi.org\/10.1038\/icb.1983.22","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1983,4]]}}}