{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T08:11:49Z","timestamp":1696061509953},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[1983,12,1]],"date-time":"1983-12-01T00:00:00Z","timestamp":439084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1983,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>An enzyme\u2010linked immunoassay was developed for diagnosing enteric fever. The test measured the inhibition of binding between a labelled, monoclonal IgM antibody and the insolubilized antigen, <jats:italic>Salmonella typhi<\/jats:italic> lipopolysaccharide (LPS). Good discrimination was seen between 32 proven typhoid cases (88\u00b70 \u00b1 4\u00b74% inhibition) and non\u2010typhoid cases. The latter consisted of 27 febrile patients bacteriologically and serologically (Widal test) found to be negative for typhoid (26\u00b73 \u00b1 10\u00b78% inhibition), 46 patients screened for syphilis (VDRL test) but found negative (31\u00b72 \u00b1 13\u00b73% inhibition), and 27 healthy blood donors (44\u00b76 \u00b1 13\u00b79% inhibition). The test also efficiently delected all 5 known typhoid carriers (90\u00b76 \u00b1 3\u00b74% inhibition). The antibody binds antigen 9 in the LPS; however, this reaction was inhibited by antibodies directed against both this antigen and an adjacent antigen, 12. Anti\u201012 antibodies presumably inhibit by steric hindrance and their importance in the test is discussed. Thus, the assay potentially detects (only) those systemic infections caused by salmonellae that possess antigen 9 or 12 (viz. <jats:italic>S. typhi<\/jats:italic> and <jats:italic>S. paratyphi A<\/jats:italic>).<\/jats:p>","DOI":"10.1038\/icb.1983.65","type":"journal-article","created":{"date-parts":[[2007,8,13]],"date-time":"2007-08-13T13:17:23Z","timestamp":1187011043000},"page":"687-704","source":"Crossref","is-referenced-by-count":13,"title":["DIAGNOSIS OF ENTERIC FEVER BY INHIBITION ASSAY USING PEROXIDASE\u2010LABELLED MONOCLONAL ANTIBODY AND <i>SALMONELLA TYPHI<\/i> LIPOPOLYSACCHARIDE"],"prefix":"10.1111","volume":"61","author":[{"given":"Pak\u2010Leong","family":"Lim","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Hong Kong, Queen Mary Hospital Compound Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi\u2010Yee","family":"Ho","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Hong Kong, Queen Mary Hospital Compound Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1983,12]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1983.65","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198365","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198365.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1983.65","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb198365.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T11:46:44Z","timestamp":1695988004000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1983.65"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1983,12]]}},"alternative-id":["10.1038\/icb.1983.65"],"URL":"https:\/\/doi.org\/10.1038\/icb.1983.65","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1983,12]]}}}