{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T09:16:47Z","timestamp":1696065407225},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1984,2,1]],"date-time":"1984-02-01T00:00:00Z","timestamp":444441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1984,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The glucose and oxygen consumption, glycolytic ratio, lactate and pyruvate production and change in adenylate energy charge (AEC) and ATP concentration of B strain <jats:italic>B. bovis<\/jats:italic>\u2010infected erythrocytes were measured. Although less than 5% of erythrocytes were infected, glucose and oxygen consumption in basal salts solution (BSS) were more than 2\u00b75 times, and pyruvate production 7 times, those of uninfected erythrocytes. The glucose consumption of <jats:italic>B. bovis<\/jats:italic> alone was similar to previously reported values for <jats:italic>B. rodhaini<\/jats:italic> alone but the glycolytic ratio of <jats:italic>B. bovis<\/jats:italic> was less than half that previously reported for <jats:italic>B. rodhaini.<\/jats:italic><\/jats:p><jats:p>Pre\u2010incubation concentrations of ATP in B strain <jats:italic>B. bovis<\/jats:italic>\u2010infected erythrocytes were significantly lower than in uninfected erythrocytes but increased considerably following incubation in BSS, in contrast to <jats:italic>B. rodhaini<\/jats:italic> in which ATP concentrations were previously shown to decrease significantly following incubation in basal salts medium.<\/jats:p><jats:p>When monitored every 4 h during 24 h incubation in BSS, the glucose consumption and lactate production of two strains of <jats:italic>B. bovis<\/jats:italic> were very similar. The glycolytic ratio of K strain <jats:italic>B. bovis<\/jats:italic> in RPMI 1640 medium plus 15% bovine plasma was double that in BSS. Both strains of <jats:italic>B. bovis<\/jats:italic> were infective after 24 h incubation in basal medium, in marked contrast to <jats:italic>B. rodhaini<\/jats:italic> which had been shown to lose infectivity.<\/jats:p>","DOI":"10.1038\/icb.1984.5","type":"journal-article","created":{"date-parts":[[2007,8,9]],"date-time":"2007-08-09T11:27:04Z","timestamp":1186658824000},"page":"53-61","source":"Crossref","is-referenced-by-count":5,"title":["METABOLISM OF <i>BABESIA<\/i> PARASITES <i>IN VITRO<\/i>. GLUCOSE AND ENERGY METABOLISM OF <i>B. BOVIS<\/i>"],"prefix":"10.1111","volume":"62","author":[{"given":"DN","family":"Barry","sequence":"first","affiliation":[{"name":"Queensland Department of Primary Industries, Animal Research Institute Yeerongpilly Queensland 4105 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1984,2]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1984.5","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb19845","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb19845.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1984.5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb19845.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T16:20:41Z","timestamp":1696004441000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1984.5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,2]]}},"alternative-id":["10.1038\/icb.1984.5"],"URL":"https:\/\/doi.org\/10.1038\/icb.1984.5","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1984,2]]}}}