{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T09:15:08Z","timestamp":1696065308359},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1984,2,1]],"date-time":"1984-02-01T00:00:00Z","timestamp":444441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1984,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Studies were undertaken to investigate a possible correlation between the change, during experimental <jats:italic>in vitro<\/jats:italic> treatments, in adenylate energy charge (AEC) of <jats:italic>B. rodhaini<\/jats:italic>\u2010infected erythrocytes and the infectivity of the parasites for mice.<\/jats:p><jats:p>When the AEC and infectivity of <jats:italic>B. rodhaini<\/jats:italic>\u2010infected erythrocytes were modified by incubation in basal salts media containing various substrates, parasite infectivity correlated significantly (r<jats:sup>2<\/jats:sup> = 0\u00b783, p&lt;0\u00b701) with change in AEC. However, <jats:italic>B. rodhaini<\/jats:italic> frozen with and without cryoprotectant demonstrated large changes in infectivity but relatively small changes in AEC. The final AEC values were similar to those of unfrozen organisms, although infectivity was greatly depressed.<\/jats:p><jats:p>The results suggest that, when <jats:italic>in vitro<\/jats:italic> treatment changed the adenine nucleotide pool (ATP + ADP + AMP) of infected erythrocytes, change in AEC correlated with infectivity. When the adenylate pool was unchanged, no such correlation was evident. Thus, the AEC can be a useful index of parasite infectivity in some, but not necessarily in all, <jats:italic>in vitro<\/jats:italic> systems.<\/jats:p><jats:p>Data on the relative utilization by <jats:italic>B. rodhaini<\/jats:italic> of various substrates, obtained when AEC and infectivity of infected erythrocytes were modified, are also reported and discussed.<\/jats:p>","DOI":"10.1038\/icb.1984.6","type":"journal-article","created":{"date-parts":[[2007,8,9]],"date-time":"2007-08-09T11:27:04Z","timestamp":1186658824000},"page":"63-71","source":"Crossref","is-referenced-by-count":1,"title":["METABOLISM OF <i>BABESIA<\/i> PARASITES <i>IN VITRO<\/i>. CHANGE IN ADENYLATE ENERGY CHARGE AND INFECTIVITY OF <i>BABESIA RODHAINI<\/i>\u2010INFECTED ERYTHROCYTES"],"prefix":"10.1111","volume":"62","author":[{"given":"DN","family":"Barry","sequence":"first","affiliation":[{"name":"Queensland Department of Primary Industries, Animal Research Institute Yeerongpilly Queensland 4105 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1984,2]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1984.6","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb19846","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb19846.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1984.6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb19846.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T16:20:42Z","timestamp":1696004442000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1984.6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,2]]}},"alternative-id":["10.1038\/icb.1984.6"],"URL":"https:\/\/doi.org\/10.1038\/icb.1984.6","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1984,2]]}}}