{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:16:00Z","timestamp":1777698960759,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[1986,12,1]],"date-time":"1986-12-01T00:00:00Z","timestamp":533779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aust J Exp Biol Med"],"published-print":{"date-parts":[[1986,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The uptake (total radioactivity in intact cells) and incorporation (radioactivity bound to acid\u2010precipitable material) of <jats:sup>14<\/jats:sup>C\u2010chlorpromazine (CPZ) and <jats:sup>14<\/jats:sup>C\u2010thiouracil (TU) were studied using a library of 3 human fibroblast strains and 13 tumour cell lines. In contrast to previous studies using rodent melanomas <jats:italic>in vivo<\/jats:italic>, the melanoma lines, including lines with high tyrosinase and melanin contents, did not take up more CPZ and TU than non\u2010melanoma cells (fibroblasts, HeLa cells). Incorporation of CPZ was also broadly similar in all cell types studied. TU was selectively incorporated into the melanoma line having a high tyrosinase and melanin content but not into lines with high tyrosinase activity and low melanin content. While supporting the possibility of selective therapy for heavily\u2010pigmented melanomas using labelled TU derivatives, these results suggest that the action of potentially melanoma\u2010affined compounds should be further evaluated in human cells. Unlabelled CPZ or TU was not selectively toxic to melanoma cells. Unexpectedly, methylation\u2010sensitive tumour cells (Mer<jats:sup>\u2010<\/jats:sup> phenotype) were highly resistant to TU, thus providing a new experimental tool for understanding the genesis of this phenotype <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/icb.1986.56","type":"journal-article","created":{"date-parts":[[2007,8,7]],"date-time":"2007-08-07T16:06:46Z","timestamp":1186502806000},"page":"517-526","source":"Crossref","is-referenced-by-count":12,"title":["ACCUMULATION OF CHLORPROMAZINE AND THIOURACIL BY HUMAN MELANOMA CELLS IN CULTURE"],"prefix":"10.1111","volume":"64","author":[{"given":"PG","family":"Parsons","sequence":"first","affiliation":[{"name":"Queensland Institute of Medical Research, Herston Queensland 4006"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BJ","family":"Allen","sequence":"additional","affiliation":[{"name":"Applied Physics Division, Australian Atomic Energy Commission Research Establishment Sutherland N.S.W. 2232"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1986,12]]},"container-title":["Australian Journal of Experimental Biology and Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1986.56","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198656","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb198656.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1986.56","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb198656.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T19:09:02Z","timestamp":1696014542000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1986.56"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1986,12]]}},"alternative-id":["10.1038\/icb.1986.56"],"URL":"https:\/\/doi.org\/10.1038\/icb.1986.56","archive":["Portico"],"relation":{},"ISSN":["0818-9641"],"issn-type":[{"value":"0818-9641","type":"print"}],"subject":[],"published":{"date-parts":[[1986,12]]}}}