{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T21:18:35Z","timestamp":1698009515398},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1989,8,1]],"date-time":"1989-08-01T00:00:00Z","timestamp":617932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[1989,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>This report describes the results of functional studies on the CD3<jats:sup>+<\/jats:sup> 197<jats:sup>+<\/jats:sup> CD4<jats:sup>\u2212<\/jats:sup> CD8<jats:sup>\u2212<\/jats:sup> TCR \u03b3\/\u03b4<jats:sup>+<\/jats:sup> peripheral T lymphocyte of sheep. Cell types were identified by immunofluorescent and immunoenzymic staining and separated by fluorescence activated flow cytometry. Newly synthesized DNA was labelled <jats:italic>in vivo<\/jats:italic> by incorporation of 5\u2010bronio\u20102\u2010deoxyuridine (BrdU), and total DNA by <jats:italic>in vitro<\/jats:italic> incubation with propidium iodide. Cells were challenged <jats:italic>in vivo<\/jats:italic> with alloantigens, <jats:italic>in vitro<\/jats:italic> with alloantigens or a range of mitogens, and activation\/differentiation was assessed by determination of cell number, phenotype, and thymidine incorporation. In normal lambs the 197<jats:sup>+<\/jats:sup> cells showed no <jats:italic>in vivo<\/jats:italic> DNA synthesis except at a low level in the ileocaecal lymph node. A similarly low level of synthesis in the prescapular node was induced by local allogeneic challenge. A higher proportion of 197<jats:sup>+<\/jats:sup> cells than of other T cells isolated from blood and lymph had G2\/M phase DNA content, while very few had S phase DNA content. The response of 197<jats:sup>+<\/jats:sup> cells in terms of change in relative numbers following <jats:italic>in vivo<\/jats:italic> allogeneic challenge was quite different to that of CD4<jats:sup>+<\/jats:sup> or CD8<jats:sup>+<\/jats:sup> cells. Instead of rising to a peak at 5\u20108 days after challenge and then declining, the percentage of 197<jats:sup>+<\/jats:sup> cells rose steadily with no evidence of decline by 14 days. Purified 197<jats:sup>+<\/jats:sup> cells were activated <jats:italic>in vitro<\/jats:italic> by phytohaemagglutinin (PHA) and concanavalin\u2010A (Con\u2010A) but not by B cell mitogens; such activation was dependent on the inclusion of feeder cells or interleukin\u20102 (IL\u20102). PHA\u2010induced proliferation was accompanied by altered expression of surface molecules by a proportion of the cells. Changes observed included loss of 197 antigen and gain of CD4, CD8 or MHC II antigens. In some cases coexpression of two or more of these surface antigens occurred. Thus the 197<jats:sup>+<\/jats:sup> T\u2010cell is as distinctive in the pattern of its functional behaviour as it is in its previously reported phenotype, distribution and ontogeny.<\/jats:p>","DOI":"10.1038\/icb.1989.34","type":"journal-article","created":{"date-parts":[[2007,7,20]],"date-time":"2007-07-20T07:41:37Z","timestamp":1184917297000},"page":"223-231","source":"Crossref","is-referenced-by-count":17,"title":["Functional characteristics of 197<sup>+<\/sup> CD4<sup>\u2212<\/sup> CD8<sup>\u2212<\/sup> sheep T lymphocytes: Expansion and differentiation of peripheral T cells"],"prefix":"10.1111","volume":"67","author":[{"given":"SJ","family":"McClure","sequence":"first","affiliation":[{"name":"Basel Institute for Immunology Grenzacherstrasse 487 CH\u20104058 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"WR","family":"Hein","sequence":"additional","affiliation":[{"name":"Basel Institute for Immunology Grenzacherstrasse 487 CH\u20104058 Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1989,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90072-0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.1.2.2956146"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.3.802"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.165.4.1076"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830160105"},{"key":"e_1_2_1_7_1","first-page":"449","volume-title":"Differentiation Antigens in Lymphohemopoietic Tissues","author":"Miyasaka M","year":"1988"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830170912"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1989.33"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-442703-7.50030-9"},{"key":"e_1_2_1_11_1","first-page":"393","article-title":"Analysis of cell division among subpopulations of lymphoid cells in sheep. 1. 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Aller."},{"key":"e_1_2_1_16_1","first-page":"46","volume-title":"Immunology of the Sheep","author":"Cahill RNP","year":"1985"},{"key":"e_1_2_1_17_1","first-page":"395","article-title":"The persistent PHA\u2010responsive population in the mouse thymus. II. Recirculatory characteristics and immunological properties","volume":"32","author":"Elliott EV","year":"1977","journal-title":"Immunology"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/335730a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/335443a0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.4049\/jimmunol.141.7.2200","article-title":"Avian T cells expressing \u03b3\u03b4 receptors localize in the splenic sinusoids and the intestinal epithelium","volume":"141","author":"Bucy RP","year":"1988","journal-title":"J. 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