{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T16:37:52Z","timestamp":1765039072594},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":10066,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunology &amp; Cell Biology"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Lymphocyte activation was examined by interleukin\u20102 (IL\u20102) receptor expression on peripheral blood mononuclear cells from coeliac and control subjects. Purified T cells were incubated with gluten fraction 111 (a known toxic peptide for coeliac subjects), soyabean hydrolysate (an unrelated hydrolysed food antigen), and Concanavalin\u2010A (Con\u2010A, a non\u2010specific mitogen). After 1\u20135 days incubation, expression of IL\u20102 receptors was assessed using a cellular enzyme\u2010linked immunosorbent assay (ELISA). Gluten fraction 111 induced expression of IL\u20102 receptors on T lymphocytes from coeliac but not from normal subjects (<jats:italic>P<\/jats:italic>= 0\u00b70005), whereas soyabean hydrolysate did not induce IL\u20102 receptor expression. Lymphocytes from both coeliac and normal subjects had similar increased IL\u20102 receptor expression after incubation with Con\u2010A. Flow cytometry was also used to confirm specific expression of IL\u20102 receptor expression of lymphocytes from coeliac subjects. Interlcukin\u20102 receptor expression increased from 0 to 5\u00b74% of cultured mononuclear cells after 7 days incubation with gluten fraction III. These cells were CD3\u2010positive and CD4\u2010positive. We conclude that peripheral blood lymphocytes from coeliac subjects are sensitized specifically to gluten fraction III.<\/jats:p>","DOI":"10.1038\/icb.1990.22","type":"journal-article","created":{"date-parts":[[2007,7,6]],"date-time":"2007-07-06T11:17:13Z","timestamp":1183720633000},"page":"155-160","source":"Crossref","is-referenced-by-count":12,"title":["Lymphocyte activation as measured by interleukin\u20102 receptor expression to gluten fraction 111 in coeliac disease"],"prefix":"10.1111","volume":"68","author":[{"given":"I. A.","family":"Penttila","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. E.","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. D.","family":"Forrest","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. G.","family":"Cummins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. T.","family":"LaBrooy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,22]]},"reference":[{"key":"e_1_2_2_2_2","first-page":"1663","article-title":"Studies of intestinal lymphoid tissue. XI\u2010The immunopathology of cell mediated reactions in gluten sensitivity and other enteropathies","volume":"2","author":"Marsh M. N.","year":"1988","journal-title":"Scand. Microsc."},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(59)92051-3"},{"key":"e_1_2_2_4_2","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1136\/gut.28.4.426","article-title":"Cellular hypersensitivity to gluten derived peptides in coeliac disease","volume":"27","author":"Guan R.","year":"1987","journal-title":"Gut"},{"key":"e_1_2_2_5_2","first-page":"225","volume-title":"Immunopathology of the Small Intestine","author":"Ferguson A.","year":"1987"},{"key":"e_1_2_2_6_2","first-page":"219","article-title":"Lymphocyte subpopulations in the human small intestine. The findings in normal mucosa and in the mucosa of patients with adult coeliac disease","volume":"52","author":"Selby W. S.","year":"1983","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_2_7_2","first-page":"101","article-title":"The effect of gluten on HLA\u2010DR in the small intestinal epithelium of patients with coeliac disease","volume":"63","author":"Ciclitira P. J.","year":"1986","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_2_8_2","first-page":"574","article-title":"Hypersensitivity in the small intestinal mucosa. V. Induction of cell mediated immunity to dietary gluten","volume":"43","author":"Mowat A. M.","year":"1981","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.2.5650.159"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(76)92406-5"},{"key":"e_1_2_2_11_2","first-page":"977","article-title":"Response to wheat antigens in in vitro lymphocyte transformation among HLA\u2010B8 positive normal donors","volume":"10","author":"Cunningham\u2010Rundles S.","journal-title":"Transplant. Proc"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(88)90422-2"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.23.2.115"},{"key":"e_1_2_2_14_2","doi-asserted-by":"crossref","first-page":"89P","DOI":"10.1042\/cs072089Pa","article-title":"Preliminary characterisation of leucocyte migration inhibition factor (LMIF) produced by lymphocytes from coeliac patients stimulated with gluten peptides","volume":"72","author":"Lydford\u2010Davis H.","year":"1987","journal-title":"Clin. Sci"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.154.5.1455"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90114-1"},{"key":"e_1_2_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830170910"},{"key":"e_1_2_2_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.4.1126"},{"key":"e_1_2_2_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.19.2.126"},{"key":"e_1_2_2_20_2","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.4049\/jimmunol.138.5.1338","article-title":"Lack of IL\u20102 dependent proliferation despite significant expression of high\u2010affinity IL\u20102 receptors on murine cytolytic lymphocytes clones late after antigenic stimulation","volume":"138","author":"Churilla A.","year":"1987","journal-title":"J. Immunol."},{"key":"e_1_2_2_21_2","first-page":"345","article-title":"Serum interleukin\u20102 receptor in coeliac disease: response to treatment and gluten challenge","volume":"11","author":"Crabtree J. E.","year":"1989","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_2_22_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198407000-00004"},{"key":"e_1_2_2_23_2","first-page":"284","article-title":"Gliadin presented via the gut induces oral tolerance in mice","volume":"72","author":"Troncone R.","year":"1988","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_2_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.26.3.285"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1990.22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb199022","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb199022.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1990.22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb199022.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T01:53:30Z","timestamp":1695952410000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1990.22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["10.1038\/icb.1990.22"],"URL":"https:\/\/doi.org\/10.1038\/icb.1990.22","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"value":"0818-9641","type":"print"},{"value":"1440-1711","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]}}}