{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T05:26:19Z","timestamp":1696051579191},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":9762,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunology &amp; Cell Biology"],"published-print":{"date-parts":[[1991,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The excretory\u2010secretory (ES) antigens of the filarial parasite <jats:italic>Brugia malayi<\/jats:italic> adult (BmA) and microfilariae (MF) were analysed for differences in their protein composition by two\u2010dimensional gel electrophoresis. Both BmA and MF biosynthetically labelled with [<jats:sup>3<\/jats:sup>H]\u2010leucine released a 200 kD molecule with pi (isoelectric point) ranging from 5.1 to 6.8. A monoclonal antibody (MoAb) to phosphocholine (PC) immunoprecipitated the 200 kD molecule with a PI of 5.1\u20135.3 from both BmA and MF.<\/jats:p><jats:p>Immunization of CBA\/N mice with ES antigens resulted in MoAb that reacted with PC, as well as some that did not (ES\u20101, ES\u20102 and ES\u20103). The epitopes recognized by these non\u2010PC MoAb varied greatly in their molecular weight, heat\u2010resistance, TCA\u2010solubility and metaperiodate\u2010sensitivity. which suggests a proteoglycan nature of the epitope. An antigen capture assay using ES\u2010l and ES\u20103 detected a circulating filarial antigen not only in patients infected with <jats:italic>Wuchereria bancrofti<\/jats:italic> (77\u201383% of those with asymptomatic microfilaraemia and 52\u201360% of those with chronic lymphatic obstruction) but also in serum from patients infected with other helminth parasites 12\u2013100%). Indeed, the epitope recognized by these MoAb was also present on other helminth parasites, which suggests conservation of a carbohydrate\u2010like prosthetic group(s) on diverse helminth species.<\/jats:p>","DOI":"10.1038\/icb.1991.19","type":"journal-article","created":{"date-parts":[[2007,7,4]],"date-time":"2007-07-04T14:02:27Z","timestamp":1183557747000},"page":"127-133","source":"Crossref","is-referenced-by-count":1,"title":["Monoclonal antibodies to secreted antigens of <i>Brugia malayi<\/i> define a cross\u2010reactive non\u2010phosphocholine determinant on helminth parasites"],"prefix":"10.1111","volume":"69","author":[{"given":"RENU B.","family":"LAL","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,22]]},"reference":[{"key":"e_1_2_2_2_2","first-page":"108","article-title":"Immunological diagnosis of human filariasis: present possibilities, difficulties and limitations (a review)","volume":"31","author":"Ambriose T. 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