{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T09:26:54Z","timestamp":1763544414865},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":8666,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunology &amp; Cell Biology"],"published-print":{"date-parts":[[1994,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The Golgi auto\u2010antigens recognized by five human autoimmune sera were characterized with anti\u2010Golgi auto\u2010antibodies. The five sera showed strong anti\u2010Golgi reactivity, together with weak anti\u2010nuclear reactivity, as assessed by indirect immunofluorescence using the human HEp2 epithelial cell line. The Golgi auto\u2010antigens recognized by the autoimmune sera were identified by immunoprecipitation and immunoblotting of post\u2010nuclear supernatants. These sera reacted with at least 14 components of molecular mass (<jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub>) 35\u2013260 kDa; three components were detected only by immunoprecipitation, six components detected only by immunoblotting and five components appear to be detected by both techniques. We have previously shown that a patient with Sj\u00f6gren's syndrome has auto\u2010antibodies specific for a 230 kDa Golgi auto\u2010antigens; a bacterial fusion protein containing auto\u2010epitope(s) of this Golgi auto\u2010antigen is not recognized by the other four autoimmune sera. Taken together, this study shows that the five anti\u2010Golgi autoimmune sera recognize distinct sets of auto\u2010antigens which include both conformational and\/or sequential epitopes.<\/jats:p>","DOI":"10.1038\/icb.1994.19","type":"journal-article","created":{"date-parts":[[2007,7,3]],"date-time":"2007-07-03T09:15:03Z","timestamp":1183454103000},"page":"123-127","source":"Crossref","is-referenced-by-count":19,"title":["Heterogeneity of human anti\u2010Golgi auto\u2010antibodies: Reactivity with components from 35 to 260 kDa"],"prefix":"10.1111","volume":"72","author":[{"given":"JACOBA","family":"KOOY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BAN\u2010HOCK","family":"TOH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PAUL A.","family":"GLEESON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60641-0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114127"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90356-4"},{"key":"e_1_2_1_5_2","first-page":"579","article-title":"Anti\u2010Golgi complex autoantibodies in a patient with Sj\u00f6gren's syndrome and lymphoma","volume":"49","author":"Rodriguez J. L.","year":"1982","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"2904","DOI":"10.4049\/jimmunol.132.6.2904","article-title":"Antibodies from patients with autoimmune disease react with a cytoplasmic antigen in the Golgi complex","volume":"132","author":"Fritzler M. J.","year":"1984","journal-title":"J Immunol."},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009748809098799"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/S0021-9258(19)88694-5","article-title":"Human autoantibodies as reagents to conserved Golgi components. Characterisation of a peripheral, 230 kDa compartment\u2010specific Golgi protein.","volume":"267","author":"Kooy J.","year":"1992","journal-title":"J. Biol. 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