{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:44:55Z","timestamp":1773661495755,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":8360,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunology &amp; Cell Biology"],"published-print":{"date-parts":[[1995,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The increased susceptibility to homologous complement in paroxysmal nocturnal haemoglobinuria (PNH) is known to be associated with the deficiency of the membrane complement inhibitors CD59 and CD55. Proteases have been used in this study to modify normal human RBC to complement sensitive PNH\u2010like cells. To investigate the protective role of CD59 and CD55, the relationship between the content of CD59 and CD55 and the complement susceptibility of the PNH\u2010like cells has been determined. The differential resistance of the enzyme\u2010treated RBC to complement\u2010mediated injury was measured by acidified serum lysis. Pronase\u2010treated erythrocytes lacked both CD59 and CD55 and were very susceptible to complement\u2010mediated lysis. Papain treatment of RBC reduced the CD55 content but did not affect CD59 and induced slight susceptibility to complement\u2010mediated lysis. Trypsin treatment of RBC destroyed 80% of CD59, had little effect on CD55 (unless incubation was extended) and slightly increased susceptibility to lysis. Thus, partial CD55 and CD59 activity was sufficient to protect cells from complement\u2010mediated lysis. In the reactive lysis assay, anti\u2010CD55 and anti\u2010CD59 induced haemolysis, anti\u2010CD59 having the more pronounced effect. Lysis was enhanced when RBC were treated by both antibodies simultaneously.<\/jats:p>","DOI":"10.1038\/icb.1995.11","type":"journal-article","created":{"date-parts":[[2007,7,3]],"date-time":"2007-07-03T09:01:03Z","timestamp":1183453263000},"page":"66-72","source":"Crossref","is-referenced-by-count":11,"title":["Protease\u2010modified erythrocytes: CD55 and CD59 deficient PNH\u2010like cells"],"prefix":"10.1111","volume":"73","author":[{"given":"FANG F.","family":"YUAN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JENNIFER A.","family":"BRYANT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANNE","family":"FLETCHER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.11.5867"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.163.4.837"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"184","DOI":"10.4049\/jimmunol.129.1.184","article-title":"Isolation of a human erythrocyte membrane glycoprotein with decay accelerating activity for C3 convertases of the complement system","volume":"129","author":"Nicholson\u2010Weller A.","year":"1982","journal-title":"J. Immunol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.4049\/jimmunol.136.5.1772","article-title":"Homologous species restriction in lysis of human erythrocytes: A membrane derived protein with C8\u2010binding capacity functions as an inhibitor","volume":"136","author":"Sch\u00f6nermark S.","year":"1986","journal-title":"J. Immunol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.18.6975"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2650471"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1992.tb02048.x"},{"key":"e_1_2_1_9_2","first-page":"33","article-title":"CD59: A molecule involved in antigen presentation as well as downregulation of membrane attack complex","volume":"9","author":"Venneker G. T.","year":"1992","journal-title":"Exp. Clin. Immunogenet."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.17.5430"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.5.1558"},{"key":"e_1_2_1_12_2","first-page":"1","article-title":"Human protectin (CD59), an 18,000\u201320,000 MW complement lysis restricting factor, inhibits C5b\u20108 catalysed insertion of C9 into lipid bilayers","volume":"71","author":"Meri S.","year":"1990","journal-title":"Immunology"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.144.9.3478"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"4941","DOI":"10.4049\/jimmunol.151.9.4941","article-title":"Interactions of soluble CD59 with the terminal complement complexes: CD59 and C9 compete for a nascent epitope on C8","volume":"151","author":"Lehto T.","year":"1993","journal-title":"J. Immunol"},{"key":"e_1_2_1_15_2","first-page":"585","article-title":"The C8\u2010binding protein of human erythrocytes: Interaction with the components of the complement\u2010attack phase","volume":"66","author":"Sch\u00f6nermark S.","year":"1988","journal-title":"Immunology"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V75.8.1595.1595"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1993.331094054626.x"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7680492"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90250-T"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1991.tb08014.x"},{"key":"e_1_2_1_21_2","first-page":"507","article-title":"New monoclonal antibodies in CD59: Use for the analysis of peripheral blood cells from paroxysmal nocturnal haemoglobinuria (PNH) patients and for the quantitation of CD59 on normal and decay accelerating factor (DAF)\u2010deficient erythrocytes","volume":"15","author":"Fletcher A.","year":"1992","journal-title":"Immunology"},{"key":"e_1_2_1_22_2","first-page":"307","article-title":"A human cell\u2010surface glycoprotein that carries Cromer\u2010related blood group antigens on erythrocytes and is also expressed on leucocytes and platelets","volume":"62","author":"Spring F. A.","year":"1987","journal-title":"Immunology"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2210097"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1982.tb07295.x"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.143.7.2262"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(90)90061-4"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"2764","DOI":"10.1182\/blood.V77.12.2764.2764","article-title":"Induction of the paroxysmal nocturnal hemoglobinuria phenotype in normal human erythrocytes; Effects of 2\u2010aminoethyIisothiouronium bromide on membrane proteins that regulate complement","volume":"71","author":"Ezzell J. L.","year":"1991","journal-title":"Blood"},{"key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1182\/blood.V17.1.83.83","article-title":"PH dependent hemolytic systems. I. Their relationship to paroxysmal nocturnal haemoglobinuria","volume":"17","author":"Yachnin S.","year":"1961","journal-title":"Blood"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114311"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1989.tb00260.x"},{"key":"e_1_2_1_31_2","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1182\/blood.V74.1.437.437","article-title":"The Inab phenotype: Characterization of the membrane protein and complement regulatory defect","volume":"14","author":"Telen M. J.","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1993.tb01691.x"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199010253231707"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V78.3.820.820"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Ficb.1995.11","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb199511","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/icb199511.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/icb.1995.11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/www.nature.com\/articles\/icb199511.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T23:22:38Z","timestamp":1695943358000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/icb.1995.11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,2]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,2]]}},"alternative-id":["10.1038\/icb.1995.11"],"URL":"https:\/\/doi.org\/10.1038\/icb.1995.11","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"value":"0818-9641","type":"print"},{"value":"1440-1711","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,2]]}}}