{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T05:43:47Z","timestamp":1698644627792},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":7813,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunology &amp; Cell Biology"],"published-print":{"date-parts":[[1996,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Despite the potential for extensive diversity of \u03b3\u03b4 TCR, especially for \u03b4\u2010chains, expression of the \u03b3\u03b4 TCR repertoire in various mouse epithelial tissues is highly restricted. This implies that the recognition of antigen by \u03b3\u03b4 T cells may also be limited. To date, however, few studies have examined \u03b3\u03b4 TCR diversity in peripheral lymphoid tissue. This report presents the V\u03b4 usage and junctional region sequences of TCR \u03b4\u2010chain transcripts derived from the lymph nodes of normal (PL\/J \u00d7 SJ\/L) Fl mice. Rearranged TCR V\u03b4\u2010C\u03b4 transcripts were amplified by PCR from TCR \u03b4\u2010chain cDNA using oligonucieotide primers specific for the murine V\u03b4l to V\u03b3\u03b47 genes and the C\u03b4 region. Following cloning of the polymerase chain reaction\u2010amplified TCR \u03b4 cDNA, the extent of junctional diversity was assessed by nucleotide sequencing of the V\u2010D\u2010J junctions of individual TCR cDNA clones. With the exception of V\u03b43, all V\u03b4 genes were expressed in mouse lymph node. Furthermore, predominant usage of J\u03b41 was found in cDNA clones expressing V\u03b42. V\u03b44, V\u03b45. V\u03b46 and V\u03b47 gene segments but despite this there was extensive junctional diversity of \u03b4\u2010chains primarily due to the usage of multiple D\u03b4 segments and N\u2010nucleotide sequence addition. In contrast, the V\u03b4l cDNA clones had limited heterogeneity consisting mostly of V\u03b41 directly spliced to \u03b3\u03b4 gene rearrangements. These results show that the expressed murine peripheral TCR \u03b4\u2010chain repertoire is extremely diverse indicating that \u03b3\u03b4 T cells may potentially recognize a large number of antigens, and play an important role in contributing to host immune responses.<\/jats:p>","DOI":"10.1038\/icb.1996.56","type":"journal-article","created":{"date-parts":[[2007,6,16]],"date-time":"2007-06-16T14:04:33Z","timestamp":1182002673000},"page":"313-317","source":"Crossref","is-referenced-by-count":5,"title":["Expression of the T cell receptor \u03b4\u2010chain repertoire in mouse lymph node"],"prefix":"10.1111","volume":"74","author":[{"given":"COLLEEN","family":"OLIVE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90369-6"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.07.040189.001135"},{"key":"e_1_2_1_4_2","first-page":"57","article-title":"Molecular organization, ontogeny and expression of murine \u03b4\u03b2 and \u03b3\u03b4 T cell receptors","volume":"8","author":"Penninger J","year":"1991","journal-title":"Exp. 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