{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:20:22Z","timestamp":1759332022530},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,12,22]],"date-time":"2017-12-22T00:00:00Z","timestamp":1513900800000},"content-version":"vor","delay-in-days":7691,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunology &amp; Cell Biology"],"published-print":{"date-parts":[[1996,12]]},"abstract":"<jats:title>Summary.<\/jats:title><jats:p>The primary cytotoxicity of <jats:italic>Staphylococcal<\/jats:italic> enterotoxin B (SEB)\u2010stimulated PBMC and separated CD8<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup> T cells against Burkitt's lymphoma target cells has been characterized by applying two stimulation protocols. In the bulk protocol, the PBMC were stimulated with 100 ng\/mL SEB for 3 days before separation to CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T subsets. In the direct protocol, the separated CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells were stimulated with 100 ng SEB preabsorbed to mitomycin C (MMC)\u2010treated APC. Comparison of the results of the two different protocols revealed the following differences: (i) PBMC in the direct protocol provided greater cytolytic activity than in the bulk protocol; and (ii) the CD4<jats:sup>+<\/jats:sup> T cells acquired cytotoxicity only in the direct protocol. Unexpectedly, the superantigen\u2010dependent cellular cytotoxicity (SDCC) of SEB\u2010stimulated cells was not dominant compared with the basal cytotoxicity. The classical NK target, K\u2010562 was also sensitive to SEB\u2010augmented cytotoxicity. The parallel stimulation with IL\u20102 and SEB caused a similar extent of cytotoxicity enhancement against both types of target cells. However, the cyclosporin A (CSA) inhibited only the SEB\u2010induced cytotoxicity. The results suggest that SEB\u2010induced PBMC acquire mainly a LAK\u2010like cytotoxicity, as a consequence of newly produced lymphokines. This observation might propose a different approach in pathological studies.<\/jats:p>","DOI":"10.1038\/icb.1996.80","type":"journal-article","created":{"date-parts":[[2007,6,16]],"date-time":"2007-06-16T14:06:08Z","timestamp":1182002768000},"page":"483-489","source":"Crossref","is-referenced-by-count":3,"title":["Primary cytotoxicity of SEB\u2010activated human PBMC and separated CD8<sup>+<\/sup> and CD4<sup>+<\/sup> T lymphocytes elicited by two different stimulation protocols"],"prefix":"10.1111","volume":"74","author":[{"given":"GY\u00d6RGY","family":"SEPR\u00c9NYI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JUN","family":"ABE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TAKAO","family":"KOHSAKA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2185544"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/smim.1993.1002"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/smim.1993.1005"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/308365a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(89)90378-X"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"3873","DOI":"10.4049\/jimmunol.150.9.3873","article-title":"Study of activation of murine T cells with bacterial superantigens. 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